A5049712719- Congenital heart defects research
- Pluripotent Stem Cells Research
- Pancreatic function and diabetes
- Cardiac Fibrosis and Remodeling
- Tissue Engineering and Regenerative Medicine
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
- Atherosclerosis and Cardiovascular Diseases
- Renal and related cancers
- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- Diabetes and associated disorders
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- Signaling Pathways in Disease
- Metabolism, Diabetes, and Cancer
- Angiogenesis and VEGF in Cancer
- Epigenetics and DNA Methylation
- Cardiac Structural Anomalies and Repair
- Congenital Heart Disease Studies
- COVID-19 Clinical Research Studies
- Molecular Biology Techniques and Applications
- Cardiovascular Function and Risk Factors
- Electrospun Nanofibers in Biomedical Applications
Prince of Wales Hospital
2016-2025
Chinese University of Hong Kong
2016-2025
University of Hong Kong
2012-2025
University of Southern California
2024
Chinese University of Hong Kong, Shenzhen
2021-2023
City University of Hong Kong, Shenzhen Research Institute
2022
ShanghaiTech University
2016-2018
Shanghai Institutes for Biological Sciences
2016-2018
Jinan University
2018
Center for Excellence in Molecular Cell Science
2018
Infectious tolerance describes the process of CD4 + regulatory T cells (Tregs) converting naïve to become additional Tregs. We show that antigen-specific Tregs induce, within skin grafts and dendritic cells, expression enzymes consume at least 5 different essential amino acids (EAAs). fail proliferate in response antigen when any 1, or more, these EAAs are limiting, which is associated with a reduced mammalian target rapamycin (mTOR) signaling. Inhibition mTOR pathway by limiting EAAs,...
Significance The human placenta is a dynamic and cellular heterogeneous organ, which critical in fetomaternal homeostasis the development of preeclampsia. Previous work has shown that placenta-derived cell-free RNA increases during pregnancy. We applied large-scale microfluidic single-cell transcriptomic technology to comprehensively characterize heterogeneity placentas identified multiple placental cell-type–specific gene signatures. Analysis signature expression maternal plasma enabled...
Whether the adult mammalian heart harbors cardiac stem cells for regeneration of cardiomyocytes is an important yet contentious topic in field cardiovascular regeneration. The putative myocyte cell populations recognized without specific markers, such as cardiosphere-derived cells, or with markers Sca1+, Bmi1+, Isl1+, Abcg2+ have been reported. Moreover, it remains unclear whether unknown unidentified exist and give rise to de novo heart.To address this question relying on a particular...
Cell-free DNA (cf.DNA) is a powerful noninvasive biomarker for cancer and prenatal testing, it circulates in plasma as short fragments. To elucidate the biology of cf.DNA fragmentation, we explored roles deoxyribonuclease 1 (DNASE1), like 3 (DNASE1L3), fragmentation factor subunit beta (DFFB) with mice deficient each these nucleases. By analyzing ends fragments type nuclease-deficient those wild-type mice, show that nuclease has specific cutting preference reveals stepwise process...
The mechanisms that promote the generation of new coronary vasculature during cardiac homeostasis and after injury remain a fundamental clinically important area study in cardiovascular field. Recently, it was reported mesenchymal-to-endothelial transition (MEndoT) contributes to substantial numbers endothelial cells myocardial infarction. Therefore, MEndoT has been proposed as paradigm mediating neovascularization is considered promising therapeutic target regeneration. Here, we show...
The idea of extending the lifetime our organs is as old humankind, fueled by major advances in organ transplantation, novel drugs, and medical devices. However, true regeneration human tissue has become increasingly plausible only recent years. heart always been a focus such efforts, given its notorious inability to repair itself following injury or disease. We discuss here emerging bioengineering approaches muscle paradigm for regenerative medicine. Our on biologically inspired strategies...
Cardiac cells marked by c-Kit or Kit, dubbed cardiac stem (CSCs), are in clinical trials to investigate their ability stimulate regeneration and repair. These studies were initially motivated the purported cardiogenic activity of these cells. Recent lineage tracing using Kit promoter drive expression inducible Cre recombinase showed that CSCs had highly limited activity, inadequate support efficient Here we reassess data investigating identity immediately after labeling. Our instant approach...
Hepatocytes are functionally heterogeneous and divided into two distinct populations based on their metabolic zonation: the periportal pericentral hepatocytes. During liver injury regeneration, cellular dynamics of these remain largely elusive. Here we show that major facilitator super family domain containing 2a (Mfsd2a), previously known to maintain blood-brain barrier function, is a zonation marker. By genetic lineage tracing Mfsd2a+ hepatocytes, population decreases during homeostasis....
The neonatal mouse heart is capable of transiently regenerating after injury from postnatal day (P) 0-7 and macrophages are found important in this process. However, whether alone sufficient to orchestrate regeneration; what regulates cardiomyocyte proliferation; why cardiomyocytes do not proliferate P7; adaptive immune cells such as regulatory T-cells (Treg) influence regeneration have less studied.
Although the mammalian heart can regenerate during neonatal stage, this endogenous regenerative capacity is lost with age. Importantly, replication of cardiomyocytes has been found to be key mechanism responsible for cardiac regeneration. Unraveling transcriptional regulatory network inducing cardiomyocyte will, therefore, crucial development novel therapies drive repair after injury. Here, we investigated whether transcription factor GATA4 required mouse Using cryoinjury and apical...
Elucidation of the role different cell lineages in liver could offer avenues to drive regeneration. Previous studies showed that SOX9+ hepatocytes can differentiate into ductal cells after injuries. It is unclear whether are uni- or bipotent progenitors at a single-cell level during injury. Here, we developed genetic tracing system delineate lineage potential homeostasis and Fate-mapping data these respond specifically injuries, with some contributing substantial number cells. Clonal...
Abstract Atherosclerosis is initiated with endothelial cell (EC) dysfunction and vascular inflammation under hyperlipidemia. Sirtuin 3 (SIRT3) a mitochondrial deacetylase. However, the specific role of SIRT3 during atherosclerosis remains poorly understood. The present study aims to mechanism in EC function atherosclerosis. Wild‐type Sirt3f/f mice endothelium‐selective knockout Sirt3f/f; Cdh5Cre/+ (Sirt3 EC‐KO ) are injected adeno‐associated virus (AAV) overexpress PCSK9 fed high‐cholesterol...
Nuclear erythroid 2-related factor 2 (Nrf2), a transcription factor, is critically involved in the regulation of oxidative stress and inflammation. However, role endothelial Nrf2 atherogenesis has yet to be defined. In addition, how activated whether can targeted for prevention treatment atherosclerosis not explored.
Distinct families of multipotent heart progenitors play a central role in the generation diverse cardiac, smooth muscle and endothelial cell lineages during mammalian cardiogenesis. The identification precise paracrine signals that drive cell-fate decision these progenitors, development novel approaches to deliver vivo, are critical steps towards unlocking their regenerative therapeutic potential. Herein, we have identified family human cardiac intermediates located outflow tract early fetal...
Human pluripotent stem cell (hPSC)-derived endothelial lineage cells constitutes a promising source for therapeutic revascularization, but progress in this arena has been hampered by lack of clinically-scalable differentiation protocols and inefficient formation functional vessel network integrating with the host circulation upon transplantation. Using human embryonic reporter line, where green fluorescent protein expression is driven an cell-specific VE-cadherin (VEC) promoter, we screened...