A5047602445- Atrial Fibrillation Management and Outcomes
- Cardiac Arrhythmias and Treatments
- Cardiac Imaging and Diagnostics
- Cardiac pacing and defibrillation studies
- Cardiac electrophysiology and arrhythmias
- Cardiac Structural Anomalies and Repair
- Pluripotent Stem Cells Research
- Cardiovascular Function and Risk Factors
- Tissue Engineering and Regenerative Medicine
- Advanced MRI Techniques and Applications
- T-cell and B-cell Immunology
- 3D Printing in Biomedical Research
- CRISPR and Genetic Engineering
- Infective Endocarditis Diagnosis and Management
- Cardiac Valve Diseases and Treatments
- Congenital heart defects research
- Acute Myocardial Infarction Research
- DNA Repair Mechanisms
- Medical Imaging Techniques and Applications
- Immune Cell Function and Interaction
- Takotsubo Cardiomyopathy and Associated Phenomena
- Transplantation: Methods and Outcomes
- Cardiac tumors and thrombi
- Veterinary Pharmacology and Anesthesia
- Hemoglobinopathies and Related Disorders
Harvard University
2011-2025
Massachusetts General Hospital
2016-2025
Harvard University Press
2007-2022
Gordon Center for Medical Imaging
2017
Harvard Stem Cell Institute
2007-2010
Cleveland Foundation
2009
Stanford University
2005-2009
Cleveland Clinic
2009
Columbia University
2009
University of Pennsylvania
2009
In embryonic stem (ES) cells, bivalent chromatin domains with overlapping repressive (H3 lysine 27 tri-methylation) and activating 4 histone modifications mark the promoters of more than 2,000 genes. To gain insight into structure function domains, we mapped key subunits Polycomb-repressive complexes 1 2 (PRC1 PRC2) genomewide in human mouse ES cells by immunoprecipitation, followed ultra high-throughput sequencing. We find that can be segregated two classes—the first occupied both PRC2 PRC1...
Bioprinting has emerged as a promising tool in tissue engineering and regenerative medicine. Various 3D printing strategies have been developed to enable bioprinting of various biopolymers hydrogels. However, the incorporation biological factors not well explored. As importance personalized medicine is becoming more clear, need for development bioinks containing autologous/patient-specific applications becomes evident. Platelet-rich plasma (PRP) used patient-specific source autologous growth...
Abstract Chikungunya virus (CHIKV) infection causes acute disease characterized by fever, rash and arthralgia, which progresses to severe chronic arthritis in up 50% of patients. Moreover, CHIKV can be fatal infants or immunocompromised individuals has no approved therapy prevention. This phase 1, first-in-human, randomized, placebo-controlled, proof-of-concept trial conducted from January 2019 June 2020 evaluated the safety pharmacology mRNA-1944, a lipid nanoparticle-encapsulated messenger...
Mammalian preimplantation embryonic development (PED) is thought to be governed by highly conserved processes. While it had been suggested that some plasticity of signaling networks exists among different mammalian species, was not known what extent modulation the genomes and regulatory proteins could “rewire” gene (GRN) control PED. We therefore generated global transcriptional profiles from three species (human, mouse, bovine) at representative stages PED, including: zygote, two-cell,...
Background Transcatheter aortic valve replacement (TAVR) has emerged as an alternative treatment for patients with symptomatic stenosis who are at high risk surgical replacement. The development of conduction abnormalities is a major complication in the postprocedural period TAVR. Objectives objective this study was to investigate and requirement permanent pacemaker (PPM) implantation undergoing Methods Data from 137 consecutive underwent TAVR (Edwards SAPIEN valve, Edwards Lifesciences,...
During lymphocyte development, V(D)J recombination assembles antigen receptor genes from component V, D, and J gene segments. These segments are flanked by a signal sequence (RSS), which serves as the binding site for machinery. The murine Jβ2.6 segment is recombinationally inactive pseudogene, but examination of its RSS reveals no obvious reason failure to recombine. Mutagenesis demonstrates that sequences heptamer, nonamer, spacer all important. Strikingly, changes solely in can result...
Background— Sudden cardiac death among orthotopic heart transplant recipients is an important mechanism of after transplantation. The role for implantable cardioverter-defibrillators (ICDs) in this population not well established. This study sought to determine whether ICDs are effective preventing high-risk recipients. Methods and Results— We retrospectively analyzed the records all patients who had ICD implantation between January 1995 December 2005 at 5 centers. Thirty-six were considered...
Delivery of high-power short-duration radiofrequency (RF) ablation lesions is not commonly used, in part because conventional thermocouple (TC) technology underestimates tissue temperature, increasing the risk steam pop, and thrombus formation. We aimed to test whether utilization an catheter equipped with a highly accurate novel TC could facilitate safe effective delivery RF lesions.Adult male Yorkshire swine were used for study. High-power ablations (10-s total; 90 W 4 s followed by 50 6...
Established classes of high-performance electronics have driven advances in interventional biomedicine. However, the large size, planar geometry and stiff mechanical properties standard conventional employed medical devices give rise to important integration challenges with soft biological tissue. Stretchable flexible biointegrated could improve treatment procedures across a broad range applications, including cardiac, neural endovascular therapies. Here we present novel mechanics, materials...
Background Mitochondrial membrane potential (ΔΨm) arises from normal function of the electron transport chain. Maintenance ΔΨm within a narrow range is essential for mitochondrial function. Methods in vivo measurement do not exist. We use 18F-labeled tetraphenylphosphonium (18F-TPP+) to measure and map total potential, ΔΨT, as sum cellular (ΔΨc) electrical potentials. Eight pigs, five controls three with scar-like injury, were studied. Pigs studied dynamic PET scanning protocol 18F-TPP+...
Background Most published reports describing outcomes of patients with cardiovascular implantable electronic device–related infective endocarditis (CIED‐IE) are single‐center studies small patient sample sizes. The goal this study was to utilize population‐based data assess trends in CIED‐IE hospitalization and compare between hospitalized CIED‐IE, prosthetic valve (PVE), native (NVE). Methods Results A query the National (Nationwide) Inpatient Sample (NIS) database 2003 2017 identified 646...
The V(D)J recombination reaction is composed of multiple nucleolytic processing steps mediated by the recombination-activating proteins RAG1 and RAG2. Sequence analysis has suggested that RAG2 contains six kelch repeat motifs are predicted to form a six-bladed β-propeller structure, with second β-strand each demonstrating marked conservation both within between repeat-containing proteins. Here we demonstrate mutations G95R ΔI273 repeats 2 5 lead immunodeficiency in patients P1 P2. Green...
V(D)J recombination is instigated by the recombination-activating proteins RAG1 and RAG2, which catalyze site-specific DNA cleavage at border of signal sequence (RSS). Although both are required for activity, core (the catalytically active region containing residues 384–1008 1040) alone displays binding specificity conserved heptamer nonamer sequences RSS. The nonamer-binding lies near N terminus RAG1, whereas heptamer-binding has not been identified. Here, potential domains within were...