A5003765409- Epigenetics and DNA Methylation
- Cancer Immunotherapy and Biomarkers
- Ferroptosis and cancer prognosis
- Retinal Diseases and Treatments
- Autophagy in Disease and Therapy
- Advanced Glycation End Products research
- Ubiquitin and proteasome pathways
- Apelin-related biomedical research
- S100 Proteins and Annexins
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cell Adhesion Molecules Research
- Intracerebral and Subarachnoid Hemorrhage Research
- Barrier Structure and Function Studies
- Clostridium difficile and Clostridium perfringens research
- Healthcare professionals’ stress and burnout
- Pomegranate: compositions and health benefits
- Gut microbiota and health
- Drug-Induced Adverse Reactions
- Axon Guidance and Neuronal Signaling
- Tactile and Sensory Interactions
- Antiplatelet Therapy and Cardiovascular Diseases
- Kruppel-like factors research
- Posttraumatic Stress Disorder Research
- Platelet Disorders and Treatments
- GDF15 and Related Biomarkers
City University of Hong Kong
2022-2024
Chinese University of Hong Kong
2023
Fudan University
2017-2022
State Key Laboratory of Medical Neurobiology
2017-2022
Shanghai University of Traditional Chinese Medicine
2019
Neovascularization and vascular remodeling are functionally important for brain repair after stroke. We show that neutrophils accumulate in the peri-infarct cortex during all stages of ischemic Neutrophils producing intravascular intraparenchymal neutrophil extracellular traps (NETs) peak at 3-5 days. Neutrophil depletion reduces blood-brain barrier (BBB) breakdown enhances neovascularization 14 Peptidylarginine deiminase 4 (PAD4), an enzyme essential NET formation, is upregulated...
Abstract Intracerebral hemorrhage associated with thrombolytic therapy tissue plasminogen activator (tPA) in acute ischemic stroke continues to present a major clinical problem. Here, we report that infusion of tPA resulted significant increase markers neutrophil extracellular traps (NETs) the cortex and plasma mice subjected photothrombotic middle cerebral artery occlusion. Peptidylarginine deiminase 4 (PAD4), critical enzyme for NET formation, is also significantly upregulated brains...
Blood–brain barrier (BBB) breakdown and inflammation occurring at the BBB have a key, mainly deleterious role in pathophysiology of ischemic stroke. Neddylation is ubiquitylation-like pathway that critical various cellular functions by conjugating neuronal precursor cell-expressed developmentally down-regulated protein 8 (NEDD8) to target proteins. However, roles neddylation stroke remain elusive. Here, we report NEDD8 conjugation increased during acute phase after was present intravascular...
Nuclear erythroid 2-related factor 2 (Nrf2), a transcription factor, is critically involved in the regulation of oxidative stress and inflammation. However, role endothelial Nrf2 atherogenesis has yet to be defined. In addition, how activated whether can targeted for prevention treatment atherosclerosis not explored.
Background: Growth differentiation factor 11 (GDF11), a member of transforming growth factor-β (TGF-β) superfamily, was shown to rejuvenate cardiac and skeletal muscle function improve cerebral vasculature neurogenesis in old mice. However, recent experimental data reported that raising GDF11 levels inhibited regeneration had no effect on hypertrophy. Our aim investigate the effects brain repair during recovery phase after stroke. Methods: Mice were subjected distal middle artery occlusion,...
Aberrant activity of polycomb repressive complex 2 (PRC2) is involved in a wide range human cancer progression. The WD40 repeat-containing protein EED core component PRC2 and enhances through interaction with H3K27me3. In this study, we report the discovery class pyrimidone compounds, represented by BR-001, as potent allosteric inhibitors PRC2. X-ray co-crystallography showed that BR-001 directly binds H3K27me3-binding pocket. displayed antitumor potency vitro vivo. Karpas422 Pfeiffer...
Endothelial nitric oxide synthase (eNOS) monomerization and uncoupling play crucial roles in mediating vascular dysfunction diabetes, although the underlying mechanisms are still incompletely understood. Increasing evidence indicates that autophagic dysregulation is involved pathogenesis of diabetic endothelial dysfunction; however, whether autophagy regulates eNOS activity through controlling or dimerization remains elusive. In this study, flux was impaired endothelium db/db mice human...
Blood-brain barrier (BBB) defects and cerebrovascular dysfunction contribute to amyloid-β (Aβ) brain accumulation drive Alzheimer disease (AD) pathology. By regulating vascular functions inflammation in the microvasculature, a disintegrin metalloprotease with thrombospondin type I motif, member 13 (ADAMTS13) plays significant protective effect atherosclerosis stroke. However, whether ADAMTS13 influences AD pathogenesis remains unclear. Using vivo multiphoton microscopy, histological,...
The global outbreak of COVID-19 has severely affected the entire population, especially healthcare staff on frontline, who bear heavy psychosomatic burdens. A cross-sectional study was conducted with 723 participants in China from April 26 to May 9, 2020. We evaluated status, including depression, anxiety, quality life, somatic symptoms, stress, sleep disturbances, and posttraumatic stress symptoms different exposure groups. explored risk factors that affect burdens analyzed relationship...
Hyperglycemia is a crucial risk factor for cardiovascular diseases. Chronic inflammation central characteristic of obesity, leading to many its complications. Recent studies have shown that high glucose activates Yes-associated protein 1 (YAP) by suppressing AMPK activity in breast cancer cells. Metformin commonly prescribed anti-diabetic drug best known AMPK-activating effect. However, the role YAP vasoprotective effect metformin diabetic endothelial cell dysfunction still unknown. The...
Background: Exacerbated blood-brain barrier (BBB) damage is related with tissue plasminogen activator (tPA)-induced brain hemorrhage after stroke. Platelets have long been recognized as the cellular orchestrators of primary haemostasis. Recent studies demonstrated further that platelets are required for supporting intact mature blood vessels and play a crucial role in maintaining vascular integrity during inflammation. Therefore, we sought to investigate whether could reduce tPA-induced...
Triboelectric nanogenerators (TENGs) are devices that can harvest energy from mechanical motions; such be used to power wearable sensors and various low-power electronics. To increase the lifetime of device, scientists mainly use method making TENG in a hard skeleton simplify complex possible relative movements between two triboelectric parts. However, skeletons cannot embedded soft lightweight clothing. make matters worse, materials garments must able withstand high forces when worn, as...
As a major risk factor for cardiometabolic diseases, aging refers to gradual decline in physiological function, characterized with 12 conspicuous hallmarks, like telomere attrition, chronic inflammation, and dysbiosis. Common vascular hallmarks include endothelial dysfunction, inflammation. In this study, we sought test the hypothesis that young-derived gut microbiota retards metabolic impairments aged hosts. We also aimed study therapeutic efficacy of young hosts different ages. Fecal...
The detection of drug-induced anaphylactoid reactions remains a global challenge,still lacking mature and reliable animal models or test methods. Therefore,the purpose this paper is to explore establish the methods evaluation standards for that apply injection drugs. Based on reaction symptoms mice induced by intravenous drugs C48/40 Tween 80,a list systemic in was sorted out an standard established applying symptom intensity coefficient K( can represent these verity symptoms) its...
<a>Endothelial nitric oxide synthase (eNOS) monomerization and uncoupling play crucial roles in mediating vascular dysfunction diabetes mellitus although the underlying mechanisms are still incompletely understood. Growing evidence indicates that autophagic dysregulation is involved pathogenesis of diabetic endothelial dysfunction, however, whether autophagy regulates eNOS activity through controlling monomerization/dimerization remains elusive. The present study shows flux was...
This study is to explore characteristic indexes in evaluation criteria for rat skin anaphylactoid test comparing blue spot OD values at the treated position and control same animal. Common contrast agents, traditional Chinese medicine injections injections' active pharmaceutical ingredients or excipients existing clinical reaction reports were taken as drugs define K value: > 2 represents positive reaction, 1.2 ≤ represent doubtable anaphylactoid; < negative which evaluating of tested drugs....
<div>Abstract<p>Aberrant activity of polycomb repressive complex 2 (PRC2) is involved in a wide range human cancer progression. The WD40 repeat-containing protein EED core component PRC2 and enhances through interaction with H3K27me3. In this study, we report the discovery class pyrimidone compounds, represented by BR-001, as potent allosteric inhibitors PRC2. X-ray co-crystallography showed that BR-001 directly binds H3K27me3-binding pocket. displayed antitumor potency...
<div>Abstract<p>Aberrant activity of polycomb repressive complex 2 (PRC2) is involved in a wide range human cancer progression. The WD40 repeat-containing protein EED core component PRC2 and enhances through interaction with H3K27me3. In this study, we report the discovery class pyrimidone compounds, represented by BR-001, as potent allosteric inhibitors PRC2. X-ray co-crystallography showed that BR-001 directly binds H3K27me3-binding pocket. displayed antitumor potency...
<p>Data for article support. Supplementary Table 1. Antibody used ELISA and WB; 2. Primers quantitative Real-time RT-PCR; 3. BR-A selectivity across a panel of kinases; 4. EED-BR-001 Data collection refinement statistics; Figure BR-001 decreases global H3K27 methylation. Inhibition methylation in cells treated with different concentrations was measured. KATO III SNU16 were 3 days at the indicated concentrations. Levels H3K27me3 cell lysate detected using . All experiments repeated...
<p>Data for article support. Supplementary Table 1. Antibody used ELISA and WB; 2. Primers quantitative Real-time RT-PCR; 3. BR-A selectivity across a panel of kinases; 4. EED-BR-001 Data collection refinement statistics; Figure BR-001 decreases global H3K27 methylation. Inhibition methylation in cells treated with different concentrations was measured. KATO III SNU16 were 3 days at the indicated concentrations. Levels H3K27me3 cell lysate detected using . All experiments repeated...
Objectives: We have been conducting translational research in cardiovascular diseases.Acute myocardial infarction (AMI) is one of the leading causes death.Malignant ventricular arrhythmia (MVA) secondary to AMI highest risk factor sudden cardiac death.It particularly important predict MVA timely and accurately patients with AMI.This study aimed explore biomarkers reveal possible mechanisms for clinical diagnosis.Methods: Blood samples from 190 human subjects were collected differences three...
<a>Endothelial nitric oxide synthase (eNOS) monomerization and uncoupling play crucial roles in mediating vascular dysfunction diabetes mellitus although the underlying mechanisms are still incompletely understood. Growing evidence indicates that autophagic dysregulation is involved pathogenesis of diabetic endothelial dysfunction, however, whether autophagy regulates eNOS activity through controlling monomerization/dimerization remains elusive. The present study shows flux was...
Abstract Neddylation is a ubiquitylation-like pathway that critical in various cellular functions by conjugating NEDD8 to target proteins. However, the roles of neddylation stroke, remain elusive. Here, we report conjugation increased after ischemic stroke and was abundantly present neutrophils, whereas cullin-1, key substrate neddylation, upregulated endothelium. Inhibition MLN4924, inactivated cullin-RING E3 ligase (CRL), reduced brain infarction improved functional outcomes. MLN4924...