A5043283007- Systemic Lupus Erythematosus Research
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- MicroRNA in disease regulation
- Advanced Glycation End Products research
- Animal Genetics and Reproduction
- Retinal Diseases and Treatments
- Renal and related cancers
- Autophagy in Disease and Therapy
- RNA Interference and Gene Delivery
- Reproductive System and Pregnancy
- IL-33, ST2, and ILC Pathways
- Cancer-related molecular mechanisms research
- Atherosclerosis and Cardiovascular Diseases
- T-cell and B-cell Immunology
- Circular RNAs in diseases
- Xenotransplantation and immune response
- Immune Cell Function and Interaction
- Pluripotent Stem Cells Research
- Kruppel-like factors research
- Genomics, phytochemicals, and oxidative stress
- Chromosomal and Genetic Variations
- Tissue Engineering and Regenerative Medicine
- Lipid metabolism and disorders
- interferon and immune responses
Guangzhou University of Chinese Medicine
2023-2025
Jinan University
2020-2023
Shantou University
2022
Chinese University of Hong Kong
2017-2022
Shenzhen Children's Hospital
2022
Chinese University of Hong Kong, Shenzhen
2019-2022
Southern University of Science and Technology
2020
Northwest A&F University
2012-2016
Inhibition of microRNA-92a (miR-92a) is reported to suppress endothelial inflammation and delay atherogenesis. We hypothesize that miR-92a inhibition protects function through suppressing oxidative stress in diabetic db/db mice.In this study, we found elevated expression aortic endothelium from mice renal arteries subjects. Endothelial cells (ECs) exposed advanced glycation end products (AGEs) oxidized low-density lipoprotein express higher level miR-92a. Overexpression impairs...
Disturbed blood flow at arterial branches and curvatures modulates endothelial function predisposes the region to inflammation subsequent development of atherosclerotic lesions. Activation Toll-like receptors (TLRs), in particular TLR4, contributes vascular inflammation. Therefore, we investigate whether TLR4 can sense disturbed (DF) mediate inflammation.En face staining endothelium revealed that expression, activation, its downstream inflammatory markers were elevated mouse aortic arch...
Objective- TFEB (transcription factor EB) was recently reported to be induced by atheroprotective laminar flow and play an anti-atherosclerotic role inhibiting inflammation in endothelial cells (ECs). This study aims investigate whether regulates diabetic db/db mice the molecular mechanisms involved. Approach Results- Endothelial denudation shows that is mainly expressed ECs mouse aortas. Western blotting total protein level decreases whereas p-TFEB S142 (phosphorylated form of TFEB)...
Endothelial nitric oxide synthase (eNOS) monomerization and uncoupling play crucial roles in mediating vascular dysfunction diabetes, although the underlying mechanisms are still incompletely understood. Increasing evidence indicates that autophagic dysregulation is involved pathogenesis of diabetic endothelial dysfunction; however, whether autophagy regulates eNOS activity through controlling or dimerization remains elusive. In this study, flux was impaired endothelium db/db mice human...
Background Uterine corpus endometrial carcinoma (UCEC) is one of the most common cancers in women, yet lacks specific and sensitive tumor markers for diagnosis, as traditional like CA125 show limited specificity. This study investigates clinical significance prognostic value CDH18, a calcium-dependent adhesion protein linked to progression, UCEC. Methods Clinical data from UCEC patients were sourced The Cancer Genome Atlas (TCGA) database. Pan-cancer analysis, differential expression...
Contents MicroRNA (miRNA) is a kind of small non‐coding RNA molecules that function as important gene expression regulators by targeting messenger RNAs for post‐transcriptional endonucleolytic cleavage or translational inhibition. In this study, libraries were constructed based on adult dairy goat testicular tissues and sequenced using the Illumina high‐throughput sequencing technology. Blasted to miRNAs cow sheep in miRBase 19.0, 373 conserved identified testis 91 novel paired‐miRNAs found....
Abstract Objectives Acrolein ( Acr ) is a highly reactive α, β‐unsaturated aldehyde, which can induce oxygen species ROS generation. Several factors, including lipid peroxidation, clinical use of cyclophosphamide, fried foods, automobile exhausts, smoking and aging increase its concentration in blood serum. Mounting evidence has suggested that ‐induced might reduce quality sperm. Thus, the aim this study was to examine reproductive toxicity ‐caused vitro find means alleviate it. Materials...
Male germline stem cells (mGSCs), in charge for the fertility male testis, are only kind of adult that transmit genetic information to next generation, with promising prospects germplasm resources preservation and optimization, production transgenic animals. Mouse cell lines have been established valuable studying mechanisms spermatogenesis. However, there is a lack stable mGSC livestock, which restricts progress research related biotechnology. Here, we firstly an immortalized dairy goat...
ABSTRACT The balance between the self‐renewal and differentiation of male germline stem cells (mGSCs) is critical for initiation maintenance mammalian spermatogenesis. promyelocytic leukemia zinc finger (PLZF), a protein, factor maintaining mGSCs, so, evaluation PLZF pathway in mGSCs may provide deeper insight into miRNA was also an important regulating mGSCs; however, there currently no data indicating that which regulate via PLZF. Here, we predicted prospective targeting to using online...
The pathophysiology of cardiac hypertrophy is complex and multifactorial. Both the store-operated Ca 2+ entry (SOCE) excessive autophagy are major causative factors for pathological hypertrophy. However, it unclear whether these two interdependent. In this study, we examined functional role SOCE Orai1 in angiotensin II (Ang II)-induced using vitro neonatal rat cardiomyocytes (NRCMs) vivo mouse model, respectively. We show that YM-58483 or SKF-96365 mediated pharmacological inhibition SOCE,...
Previous studies have shown that promyelocytic leukaemia zinc finger (PLZF) is a spermatogonia-specific transcription factor in the testis, required to regulate self-renewal and maintenance of spermatogonia stem cell. Up now, expression function PLZF goat testis has not been known. The objectives this study were investigate pattern dairy its effect on male germline cell (mGSC) differentiation.Testis development patterns analysed by haematoxylin eosin staining, immunohistochemistry reverse...
Spermatogonia stem cells (SSCs), also named the male germline (mGSCs), which is located at base of seminiferous tubules testis, basis for generating sperm steadily in animals. Currently, there are some preliminary study on self-renewal and differentiation SSCs, but further mechanism, especially large animals, has not been clearly understood. Ras/ERK1/2 pathway widely distributed multiple vivo . It plays an important role cell proliferation, so on. However, function dairy goats SSCs...
miRNAs, a type of small RNA, play critical roles in mammalian spermatogenesis. Spermatogonia are the foundation spermatogenesis and valuable for study However, expression profiling miRNAs spermatogonia dairy goats remains unclear. CD49f has been one surface markers used enrichment by magnetic activated cell sorting (MACS). Therefore, we microbead antibody to purify CD49f-positive -negative cells goat testicular MACS then analysed miRNA these depth using Illumina sequencing technology. The...
Abstract Objective Branched‐chain amino acids (BCAAs) are popular dietary supplements for exercise. However, increased BCAA levels positively correlate with obesity and diabetes. The metabolic impact of supplementation on insulin sensitivity during exercise is less understood. Methods Male C57BL/6 mice were fed 12 weeks a high‐fat diet, normal chow or BCAA‐restricted diet. They subjected to running without treatment another weeks. Results Exercise reduced body weight, improved sensitivity,...
Abstract Background Systemic lupus erythematosus (SLE) is an autoimmune disease with a complicated pathogenesis, and its aetiology has not been clearly unveiled. The lack of effective diagnosis treatment methods makes it necessary to explore the molecular mechanism SLE. We aimed identify some critical signalling pathways key competing endogenous RNAs (ceRNAs) underlying SLE map out systematic networks by integrating data on different kinds RNAs. Methods Peripheral blood mononuclear cells...
This study was designed to investigate the effect of platelet‐derived growth factor (PDGF) on proliferation human umbilical cord mesenchymal stem cells (UC‐MSCs) and further explore mechanism PDGF in promoting UC‐MSCs. The UC‐MSCs were treated with different concentrations PDGF, effects evaluated by counting cell number, viability, expression receptors analyzed RT‐PCR, detection gene proliferation, cycle pluripotency, Brdu assay immunofluorescent staining Quantitative real‐time (QRT‐PCR)....
Previous studies have shown that promyelocytic leukemia zinc finger (PLZF), chemokine (C-X-C motif) receptor 4 (CXCR4) and mir146a were associated with the self-renewal of mouse spermatogonial stem cells (SSCs); however, there is little information on their effects fate livestock SSCs. Here, we identified a regulatory pathway in dairy goat mGSCs, involving PLZF, SDF-1 CXCR4. PLZF overexpression downregulated simultaneously upregulated expression CXCR4 protein, whereas knockdown (siPLZF)...
Systemic lupus erythematous (SLE) is a complex chronic autoimmune disease with difficult early treatment and accurate diagnosis. Circulating exosomes containing proteins, lipids nucleic acids can be ideal diagnostic biomarkers management strategies for SLE. Our aim was to examine the unique expression profiles of circulating exosomal miRNAs proteins in patients SLE patients. Using RNA-sequencing proteomic approaches, we compared patterns plasma healthy subjects, discussed underlying...
Abstract Objectives CD 49f enhances multipotency and maintains stemness in embryonic stem cells ( ESC s), however, whether it would be effective mGSC s has remained unclear. Moreover, better standards for enrichment purification are necessary. The present study was conducted to determine roles of regulation. Materials methods expression patterns were investigated dairy goats. positive purified enriched using magnetic‐activated cell sorting MACS ), characteristics the cultured assayed...