A5046817187- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- Genomics and Phylogenetic Studies
- Machine Learning in Bioinformatics
- Sexual Differentiation and Disorders
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- Urological Disorders and Treatments
- Algorithms and Data Compression
- RNA and protein synthesis mechanisms
- Grouting, Rheology, and Soil Mechanics
- RNA Interference and Gene Delivery
- Cardiac Health and Mental Health
- Geomechanics and Mining Engineering
- Pluripotent Stem Cells Research
- Cancer-related molecular mechanisms research
- Artificial Immune Systems Applications
- Bacteriophages and microbial interactions
- Testicular diseases and treatments
- Oral and Craniofacial Lesions
- Medicinal Plants and Bioactive Compounds
- Machine Learning in Healthcare
SickKids Foundation
2023-2025
University of Toronto
2025
McGill University
2025
Inserm
2020-2025
Université Côte d'Azur
2020-2025
Hospital for Sick Children
2025
Trillium Health Centre
2025
Institut de Biologie Valrose
2020-2024
Wenzhou Medical University
2023-2024
Tsinghua University
2023-2024
Gonadal sex determination represents a unique model for studying cell fate decisions. However, complete understanding of the different lineages forming developing testis and ovary remains elusive. Here, we investigated origin, specification, subsequent sex-specific differentiation previously uncharacterized population supporting-like cells (SLCs) in mouse gonads. The SLC lineage is closely related to coelomic epithelium specified as early E10.5, making it first somatic be bipotential gonad....
Depression is one of the most prevalent mental disorders today. Over past decade, there has been considerable attention given to field gut microbiota associated with depression. A substantial body research indicates a bidirectional communication pathway between and brain. In this review, we extensively detail correlation microbiota, including Lactobacillus acidophilus Bifidobacterium longum , metabolites such as short-chain fatty acids (SCFAs) 5-hydroxytryptamine (5-HT) concerning...
Male genital development in XY mammalian fetuses is triggered by the action of hormones, including testosterone, secreted developing testes. Defects this process are a cause for Differences Sex Development (DSD), one most common congenital abnormalities humans. Fetal Leydig Cells (FLC) play central role synthesis masculinizing hormones Yet, genetic cascade controlling their differentiation poorly understood. Here we investigate orphan nuclear receptor NR2F2 (COUP-TFII) FLC development. We...
Aims: Many men endure immunosuppressive or anticancer treatments that contain alkylating agents before the age of sexual maturity, especially increasing number preadolescent males who undergo busulfan treatment for myeloablative conditioning hematopoietic stem cell transplantation. Before sperm production, there are no available cryopreservation. Thus, it is necessary to identify a solution ameliorate busulfan-induced damage spermatogonial cells (SSCs). Results: In this study, we...
Abstract HAlign is a cross-platform program that performs multiple sequence alignments based on the center star strategy. Here we present two major updates of 3, which helped improve time efficiency and alignment quality, made 3 specialized to process ultra-large numbers similar DNA/RNA sequences, such as closely related viral or prokaryotic genomes. can be easily installed via Anaconda Java release package macOS, Linux, Windows subsystem for systems, source code available GitHub...
Histopathological image classification has become one of the most challenging tasks for researchers, due to varied categories and detailed differences within diseases. In this study, we investigate critical role network depth in histopathological classification, utilizing deep residual convolutional neural networks (ResNet). We evaluate efficacy two transfer learning strategies using ResNet with varying layers (18, 34, 50, 152) pretrained on ImageNet. Specifically, analyze whether a deeper...
Male genital development in XY mammalian fetuses is triggered by the action of hormones, including testosterone, secreted developing testes. Defects this process are a cause for Differences Sex Development (DSD), one most common congenital abnormalities humans. Fetal Leydig Cells (FLC) play central role synthesis masculinizing hormones Yet, genetic cascade controlling their differentiation poorly understood. Here we investigate orphan nuclear receptor NR2F2 (COUP-TFII) FLC development. We...
Abstract Background Multimorbidity is the co-occurrence of two or more chronic conditions in one individual. It associated with reduced quality life, poorer disease outcomes, increased hospitalizations, and polypharmacy. Although linked to premature mortality general populations, relationship between multimorbidity inflammatory bowel (IBD) unknown. Aims (1) characterize patterns across life course within context IBD; (2) identify how sequence condition accumulation contributes death. Methods...
<h3>Background:</h3> Multimorbidity, the co-occurrence of 2 or more chronic conditions, is important in patients with inflammatory bowel disease (IBD) given its association complex care plans, poor health outcomes, and excess mortality. Our objectives were to describe premature death (age < 75 yr) among people IBD identify patterns between multimorbidity decedents IBD. <h3>Methods:</h3> Using administrative data who died 2010 2020 Ontario, Canada, we conducted a population-based,...
Contents MicroRNA (miRNA) is a kind of small non‐coding RNA molecules that function as important gene expression regulators by targeting messenger RNAs for post‐transcriptional endonucleolytic cleavage or translational inhibition. In this study, libraries were constructed based on adult dairy goat testicular tissues and sequenced using the Illumina high‐throughput sequencing technology. Blasted to miRNAs cow sheep in miRBase 19.0, 373 conserved identified testis 91 novel paired‐miRNAs found....
Lin28a is a conserved RNA-binding protein that plays an important role in development, pluripotency, stemness maintenance, proliferation and self-renewal. Early studies showed serves as marker of spermatogonial stem cells (SSCs) promotes the capacity mouse SSCs. However, there little information about livestock In this study, we cloned Capra hircus CDS found it evolutionarily conserved. widely expressed different tissues hircus, but at high level testis. specifically located cytoplasm may...
Abstract The microbiota–gut–brain axis denotes a two-way system of interactions between the gut and brain, comprising three key components: (1) microbiota, (2) intermediates (3) mental ailments. These constituents communicate with one another to induce changes in host’s mood, cognition demeanor. Knowledge concerning regulation host central nervous by microbiota is fragmented mostly confined disorganized or semi-structured unrestricted texts. Such format hinders exploration comprehension...
Abstract Multiple sequence alignment is widely used for analysis, such as identifying important sites and phylogenetic analysis. Traditional methods, progressive alignment, are time-consuming. To address this issue, we introduce StarTree, a novel method to fast construct guide tree by combining clustering hierarchical clustering. Furthermore, develop new heuristic similar region detection algorithm using the FM-index apply k-banded dynamic program profile alignment. We also win-win that...
The transcription factors SRY and SOX9 RSPO1/WNT4/β-Catenin signaling act as antagonistic pathways to drive testis ovary development respectively, from a common gonadal primordium in mouse embryos. In this work, we took advantage of double knockout model study when Sox9 Wnt4 are both mutated. We show that the XX gonad mutant for or develop ovotestes, demonstrating ectopic function is not required partial female-to-male sex reversal caused by mutation. deletion XY gonads leads ovarian...
Abstract Motivation Multiple sequence alignment (MSA) is a fundamental problem in bioinformatics. The quality of will affect downstream analysis. MAFFT has adopted the Fast Fourier Transform method for searching homologous segments and using them as anchors to divide sequences, then making only on segments, which can save time memory without overly reducing quality. becomes slow when dataset large. Results We made software, WMSA, uses divide-and-conquer split sequences into clusters, aligns...
Abstract Objectives Ngn3 is a typical transcription factor and marker of differentiating spermatogonial stem cells ( SSC s) in mouse, belonging to the basic helix‐loop‐helix bHLH ) family. Its gene specifically expressed A type spermatogonia mouse testis, thus plays critical role controlling differentiation s. However, roles its protein dairy goat testis remain unknown. Materials methods Testis development expression patterns were analysed by immunofluorescence quantitative reverse...
A fast scanner of optical-resolution photoacoustic microscopy is inherently vulnerable to perturbation, leading severe image distortion and significant misalignment among multiple 2D or 3D images. Restoration registration these images critical for accurately quantifying dynamic information in long-term imaging. However, traditional algorithms face a great challenge computational throughput. Here, we develop an unsupervised deep learning based network achieve real-time restoration...
Abstract Objectives Nanos2, an RNA ‐binding protein, belongs to the Nanos gene‐coding family and contains two CCHC zinc‐finger motifs. In mouse, it plays a pivotal role in male germ cell development, self‐renewal of spermatogonial stem cells. However, little is known its expression pattern functions dairy goat testis. Materials methods Immunohistochemistry quantitative reverse transcription‐polymerase chain reaction ( qRT ‐ PCR ) were used generate profile Nanos2 Furthermore, overexpression...