A5054074638- Cell Image Analysis Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Single-cell and spatial transcriptomics
- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- Neurogenesis and neuroplasticity mechanisms
- Sperm and Testicular Function
- Pluripotent Stem Cells Research
- Photoreceptor and optogenetics research
- Smart Grid Energy Management
- Image Processing Techniques and Applications
- Adenosine and Purinergic Signaling
- Brain Tumor Detection and Classification
- Pain Mechanisms and Treatments
- Advanced Electron Microscopy Techniques and Applications
- Energy and Environment Impacts
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Receptor Mechanisms and Signaling
- Nerve injury and regeneration
- AI in cancer detection
- Transcranial Magnetic Stimulation Studies
- Health, Environment, Cognitive Aging
- Medical Imaging Techniques and Applications
- Traumatic Brain Injury and Neurovascular Disturbances
- Electrolyte and hormonal disorders
Wyss Center for Bio and Neuroengineering
2018-2025
The University of Melbourne
2021
European Molecular Biology Laboratory
2013-2017
University College Dublin
2009-2010
University of Bologna
2007
Gonadal sex determination represents a unique model for studying cell fate decisions. However, complete understanding of the different lineages forming developing testis and ovary remains elusive. Here, we investigated origin, specification, subsequent sex-specific differentiation previously uncharacterized population supporting-like cells (SLCs) in mouse gonads. The SLC lineage is closely related to coelomic epithelium specified as early E10.5, making it first somatic be bipotential gonad....
Neuropathic pain, caused by a lesion in the somatosensory system, is severely impairing mostly chronic disease. While its underlying molecular mechanisms are not thoroughly understood, neuroimmune interactions as well changes pain pathway such sensitization of nociceptors have been implicated. It has shown that only different cell types involved generation and maintenance neuropathic like neurons, immune glial cells, but, also, intact adjacent neurons relevant to process. Here, we describe...
The physiological role of alpha-synuclein, a protein found enriched in intraneuronal deposits characterizing Parkinson's disease, is debated. While its aggregation usually considered linked to neuropathology, normal function may be related fundamental processes synaptic transmission and plasticity. By using antisense oligonucleotide strategy, we report this study that alpha-synuclein silencing cultured cerebellar granule cells results widespread death these neurons, thus demonstrating an...
Long-term memory is formed by alterations in glutamate-dependent excitatory synaptic transmission, which turn regulated synaptosomal protein of 25 kDa (SNAP-25), a key component the soluble N-ethylmaleimide-sensitive factor attachment receptor complex essential for exocytosis neurotransmitter-filled vesicles. Both reduced and excessive SNAP-25 activity has been implicated various disease states that involve cognitive dysfunctions such as attention deficit hyperactivity disorder,...
Minibrain is a 3D brain in vitro spheroid model, composed of mixed population neurons and glial cells, generated from human iPSC derived neural stem cells. Despite the advances models such as aggregates, spheroids organoids, there lack labeling imaging methodologies to characterize these models. In this study, we present step-by-step methodology generate minibrain nurseries novel strategies subsequently label projection neurons, perform immunohistochemistry minibrains at large multiplexable...
Microglia cells, resident immune cells of the brain, survey brain parenchyma by dynamically extending and retracting their processes. Cl- channels, activated in cellular response to stretch/swelling, take part several functions deeply connected with microglia physiology, including cell shape changes, proliferation, differentiation migration. However, molecular identity functional properties these channels are largely unknown. We investigated swelling-activated currents microglial from acute...
Chloride is the most abundant physiological anion and participates in a variety of cellular processes including trans-epithelial transport, cell volume regulation, regulation electrical excitability. The development tools to monitor intracellular chloride concentration ([Cli]) therefore important for evaluation function normal pathological conditions. Recently, several Cl-sensitive genetically encoded probes have been described which allow non-invasive monitoring [Cli]. Here we describe two...
Abstract The large size of imaging datasets generated by next-generation histology methods limits the adoption those approaches in research and clinic. We propose pAPRica (pipelines for Adaptive Particle Representation image compositing analysis), a framework based on (APR) to enable efficient analysis microscopy datasets, scalable up petascale regular workstation. includes stitching, merging, segmentation, registration, mapping an atlas as well visualization 3D data, achieving 100+ fold...
We provide here a procedure enabling light sheet fluorescence microscopy (LSFM) of entire human eyes after iDISCO + -based clearing (ClearEye) and immunolabeling. Demonstrated in four eyes, post-processing LSFM stacks enables three-dimensional (3D) navigation customized display, including en face viewing the fundus similarly to clinical imaging, with resolution retinal capillaries. This method overcomes several limitations traditional histology eyes. Tracing spatially complex structures such...
Over the course of past decade, tissue clearing methods have reached a high level sophistication with wide variety approaches now available[1][1]. To image large cleared samples, light-sheet microscopes proven to be ideal due their excellent optical sectioning capability in
Projections from the lateral habenula (LHb) control ventral tegmental area (VTA) neuronal populations’ activity and both nuclei shape pathological behaviors emerging during cocaine withdrawal. However, it is unknown whether withdrawal modulates LHb neurotransmission onto subsets of VTA neurons that are part distinct circuits. Here we show that, in mice, withdrawal, drives discrete opposing synaptic adaptations at inputs defined by their output connectivity. axons innervate medial aspect VTA,...
The choroid plexus (CP) acts as a regulated gate between blood and cerebrospinal fluid (CSF). Despite its simple histology (a monostratified cuboidal epithelium overlying vascularized stroma), this organ has remarkably complex functions several of which involve local interaction with cells located around ventricle walls. Our knowledge CP structural organization is mainly derived from resin casts, capture the overall features but only allow reconstruction vascular pattern surface, unrelated...
Abstract Gonadal sex determination represents a unique model for studying cell fate decisions. However, complete understanding of the different lineages forming developing testis and ovary remains elusive. Here, we investigated origin, specification subsequent sex-specific differentiation previously uncharacterized population supporting-like cells (SLC) in mouse gonads. The SLC lineage is closely related to coelomic epithelium specified as early E10.5, making it first somatic be bipotential...
Summary With the emerging role of autophagic machinery in healthy brain development and aging, there is a pressing need to better characterize its functions different neuronal populations, providing cellular insight into autophagy-related diseases. Here, we generated characterized mice with conditional ablation atg5 GABAergic neurons expressing parvalbumin ( PV-atg5KO ), mostly comprising fast-spiking interneurons, as well Purkinje cells cerebellum. Using light-sheet microscopy image PV...
Calvarial bone marrow has been found to be central in the brain immune response, being connected dura through channels which allow leukocyte trafficking. Temporal is thought play important roles relation inner ear, but still largely uncharacterized, given this complex anatomy. We characterized geometry and connectivity of rat temporal using lightsheet imaging cleared samples microCT. Bone was identified tissue by cellular content (and particular presence megakaryocytes); since air-filled...
Abstract The growth of data throughput in optical microscopy has triggered the extensive use supervised learning (SL) models on compressed datasets for automated analysis. Investigating effects image compression SL predictions is therefore pivotal to assess their reliability, especially clinical use. We quantify statistical distortions induced by through comparison raw predictive uncertainty, numerically estimated from noise statistics measured via sensor calibration. Predictions cell...
We propose an end-to-end image analysis pipeline based on the Adaptive Particle Representation (APR) for analyzing large 3D cleared tissue samples such as whole mouse brains or human brain sections, achieving 100+ times faster computation. Our is compatible with real-time use, i.e. can be done during acquisition. In addition to processing, using APR yields memory and storage compression ratios ranging from dozens thousands depending labeling sparsity, saving costs computing infrastructures.