A5029594004- Stress Responses and Cortisol
- Neuroscience and Neuropharmacology Research
- Gastrointestinal motility and disorders
- Pain Mechanisms and Treatments
- Neurotransmitter Receptor Influence on Behavior
- Neurogenesis and neuroplasticity mechanisms
- Regulation of Appetite and Obesity
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroendocrine regulation and behavior
- Brain Tumor Detection and Classification
- Health, Environment, Cognitive Aging
- Diet and metabolism studies
- Energy and Environment Impacts
- Cell Image Analysis Techniques
- Smart Grid Energy Management
- Dendrimers and Hyperbranched Polymers
- Gut microbiota and health
- Sleep and Wakefulness Research
- Pharmacology and Obesity Treatment
- Nerve injury and regeneration
- Eating Disorders and Behaviors
- Cancer, Stress, Anesthesia, and Immune Response
- Medical Imaging Techniques and Applications
- Metabolism and Genetic Disorders
- Axon Guidance and Neuronal Signaling
University of Geneva
2020-2023
University of Fribourg
2014-2019
University of Lausanne
2011-2014
Children's Hospital of Philadelphia
2006-2010
University of Pennsylvania
2006-2010
University of California, Los Angeles
2005-2006
VA Greater Los Angeles Healthcare System
2005
Solvay (France)
2000
Centre Hospitalier Universitaire de Grenoble
2000
Chronic stress plays an important role in the development and exacerbation of symptoms functional gastrointestinal disorders. To better understand mechanisms underlying this relationship, we aimed to characterize changes visceral somatic nociception, colonic motility, anxiety-related behavior, mucosal immune activation rats exposed 10 days chronic psychological stress. Male Wistar were submitted daily either 1-h water avoidance (WA) or sham WA for consecutive days. The visceromotor response...
Stress-related psychiatric disorders such as anxiety and depression involve dysfunction of the serotonin [5-hydroxytryptamine (5-HT)] system. Previous studies have found that stress neurohormone corticotropin-releasing factor (CRF) inhibits 5-HT neurons in dorsal raphe nucleus (DRN) vivo . The goals present study were to characterize CRF receptor subtypes (CRF-R1 -R2) cellular mechanisms underlying CRF–5-HT interactions. Visualized whole-cell patch-clamp recording techniques brain slices...
Glucagon-like peptide 1 (GLP-1) and serotonin play critical roles in energy balance regulation. Both systems are exploited clinically as antiobesity strategies. Surprisingly, whether they interact order to regulate is poorly understood. Here we investigated mechanisms by which GLP-1 at the level of central nervous system. Serotonin depletion impaired ability exendin-4, a used analog, reduce body weight rats, suggesting that mediator impact receptor (GLP-1R) activation. turnover expression...
Notch signaling plays a crucial role in adult brain function such as synaptic plasticity, memory and olfaction. Several reports suggest an involvement of this pathway neurodegenerative dementia. Yet, to date, the mechanism underlying activity mature neurons remains unresolved. In work, we investigate how regulates potentiation contributes establishment mice. We observe that Notch1 is postsynaptic receptor with functional interactions Reelin receptor, apolipoprotein E 2 (ApoER2) ionotropic...
Ion imaging is a powerful methodology to assess fundamental biological processes in live cells.The limited efficiency of some ion-sensing probes and their fast leakage from cells are important restrictions this approach.In study we present novel strategy perform prolonged fluorescence intracellular ion dynamics with dendrimer-based nanoprobes.A new sodium sensitive nanoprobe was generated by encapsulating fluorescent dye PAMAM dendrimer nanocontainer.This very stable has high sensitivity...
After a peripheral nerve lesion, distal ends of injured axons disintegrate into small fragments that are subsequently cleared by Schwann cells and later macrophages. Axonal debris clearing is an early step the repair process facilitates regeneration. We show here promote cut axon disintegration for timely clearing. By combining cell-based in vivo models lesion with mouse genetics, we this mechanism induced axons, which signal to through PlGF mediating activation upregulation VEGFR1 cells. In...
Abstract The dorsal (DR) and median (MR) raphe nuclei contain 5‐hydroxytryptamine (5‐HT) cell bodies that give rise to the majority of ascending 5‐HT projections forebrain. DR MR have differential roles in mediating stress, anxiety depression. Glutamate GABA activity sculpt putative neuronal firing release a seemingly manner DR, yet isolated glutamate within has not been systematically characterized. Visualized whole‐cell voltage‐clamp techniques were used record excitatory inhibitory...
Acute stress affects gut functions through the activation of corticotropin-releasing factor (CRF) receptors. The impact acute on pelvic viscera in context chronic is not well characterized. We investigated colonic, urinary, and locomotor responses monitored as fecal pellet output (FPO), urine voiding, ambulatory activity, respectively, female male CRF-overexpressing (CRF-OE) mice, a model, their wild-type littermates (WTL). Female CRF-OE compared with WTL, had enhanced FPO to 2-min handling...
Abstract The large size of imaging datasets generated by next-generation histology methods limits the adoption those approaches in research and clinic. We propose pAPRica (pipelines for Adaptive Particle Representation image compositing analysis), a framework based on (APR) to enable efficient analysis microscopy datasets, scalable up petascale regular workstation. includes stitching, merging, segmentation, registration, mapping an atlas as well visualization 3D data, achieving 100+ fold...
SUMMARY Oxytocin orchestrates social and emotional behaviors through modulation of neural circuits in brain structures such as the central amygdala (CeA). The long-standing dogma is that oxytocin signaling nervous system occurs exclusively via direct actions on neurons. However, several findings over last decades showed astrocytes actively participate neuronal circuits. Here, we investigate degree astrocytes’ involvement functions. Using astrocyte’ specific gain loss function approaches,...
We propose an end-to-end image analysis pipeline based on the Adaptive Particle Representation (APR) for analyzing large 3D cleared tissue samples such as whole mouse brains or human brain sections, achieving 100+ times faster computation. Our is compatible with real-time use, i.e. can be done during acquisition. In addition to processing, using APR yields memory and storage compression ratios ranging from dozens thousands depending labeling sparsity, saving costs computing infrastructures.
Pinaverium bromide a GI selective calcium antagonist diminishes FOS expression in rats following nociceptive stimulus