A5077791629- Regulation of Appetite and Obesity
- Pancreatic function and diabetes
- Neuroscience and Neuropharmacology Research
- Neurotransmitter Receptor Influence on Behavior
- Adipose Tissue and Metabolism
- Biochemical Analysis and Sensing Techniques
- Receptor Mechanisms and Signaling
- Diet and metabolism studies
- Sleep and Wakefulness Research
- Neuroendocrine regulation and behavior
- Metabolism, Diabetes, and Cancer
- Neuroscience of respiration and sleep
- Neural dynamics and brain function
- Diabetes and associated disorders
- Sleep and related disorders
- Glycogen Storage Diseases and Myoclonus
- Biochemical effects in animals
- Axon Guidance and Neuronal Signaling
- Alzheimer's disease research and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Dietary Effects on Health
- Advanced MRI Techniques and Applications
- Forensic Toxicology and Drug Analysis
- Neurogenesis and neuroplasticity mechanisms
- Eating Disorders and Behaviors
University of Lausanne
2014-2024
University of British Columbia
2009-2013
University of Geneva
2007-2012
Addictive drugs have in common that they cause surges dopamine (DA) concentration the mesolimbic reward system and elicit synaptic plasticity DA neurons of ventral tegmental area (VTA). Cocaine for example drives insertion GluA2-lacking AMPA receptors (AMPARs) at glutamatergic synapes neurons. However it remains elusive which molecular target cocaine such AMPAR redistribution whether other addictive (morphine nicotine) similar changes through their effects on system.We used vitro...
Obesity has drastically increased over the last few decades. is associated with elevated insulin levels, which can gain access to brain, including into dopamine neurons of ventral tegmental area (VTA), a brain region critical for mediating reward-seeking behavior. Synaptic plasticity VTA altered motivation obtain reinforcing substances such as food and drugs abuse. Under physiological circumstances, in suppress excitatory synaptic transmission onto reduce aspects palatable feeding However,...
The counterregulatory response to hypoglycemia is an essential survival function. It controlled by integrated network of glucose-responsive neurons, which trigger endogenous glucose production restore normoglycemia. complexity this glucoregulatory is, however, only partly characterized. In a genetic screen panel recombinant inbred mice we previously identified Fgf15, expressed in neurons the dorsomedial hypothalamus (DMH), as negative regulator glucagon secretion. Here, report on generation...
Abstract The counterregulatory response to hypoglycemia that restores normal blood glucose levels is an essential physiological function. It initiated, in large part, by incompletely characterized brain sensing neurons trigger the secretion of hormones, particular glucagon, stimulate hepatic production. In a genetic screen recombinant inbred BXD mice we previously identified Agpat5 as candidate regulator hypoglycemia-induced glucagon secretion. Here, using mouse models, demonstrate expressed...
The ventromedial nucleus of the hypothalamus (VMN) is involved in counterregulatory response to hypoglycemia. VMN neurons activated by hypoglycemia (glucose-inhibited [GI] neurons) have been assumed play a critical although untested role this response. Here, we show that expression dominant negative form AMPK or inactivation α1 and α2 subunit genes Sf1 selectively suppressed GI neuron activity. We found Txn2, encoding mitochondrial redox enzyme, was strongly downregulated absence activity...
Glucokinase (Gck) is a critical regulator of glucose-induced insulin secretion by pancreatic β-cells. It has been suggested to also play an important role in glucose signaling neurons the ventromedial hypothalamic nucleus (VMN), brain involved control homeostasis and feeding. To test Gck VMN sensing physiological regulation, we studied mice with genetic inactivation gene Sf1 (Sf1Gck−/− mice). Compared littermates, Sf1Gck−/− displayed increased white fat mass adipocyte size, reduced lean...
The paraventricular nucleus of the thalamus (PVT) controls goal-oriented behavior through its connections to accumbens (NAc). We previously characterized Glut2aPVT neurons that are activated by hypoglycemia, and which increase sucrose seeking their glutamatergic projections NAc. Here, we identified glucokinase (Gck)-expressing PVT (GckaPVT) generated a mouse line expressing Cre recombinase from locus (GckCre/+ mice). Ex vivo calcium imaging whole-cell patch clamp recordings revealed GckaPVT...
Abstract Feeding behavior is a complex process that depends on the ability of brain to integrate hormonal and nutritional signals, such as glucose. One glucosensing mechanism relies glucose transporter 2 (GLUT2) in hypothalamus, especially radial glia-like cells called tanycytes. Here, we analyzed whether GLUT2-dependent required for normal regulation feeding GFAP-positive Genetic inactivation Glut2 GFAP - expressing tanycytes was performed using Cre/Lox technology . The efficiency...
Abstract The counterregulatory response to hypoglycemia (CRR), which ensures a sufficient glucose supply the brain, is an essential survival function. It orchestrated by incompletely characterized glucose‐sensing neurons, trigger coordinated autonomous and hormonal that restores normoglycemia. Here, we investigate role of hypothalamic Tmem117 , identified in genetic screen as regulator CRR. We show expressed vasopressin magnocellular neurons hypothalamus. inactivation these increases...
Proopiomelanocortin (POMC) neurons are major regulators of energy balance and glucose homeostasis. In addition to being regulated by hormones nutrients, POMC controlled glutamatergic input originating from multiple brain regions. However, the factors involved in formation inputs how they contribute bodily functions remain largely unknown. Here, we show that during development inputs, exhibit enriched expression Efnb1 (EphrinB1) Efnb2 (EphrinB2) genes, which known control excitatory synapse...
Glucose transporter 2 (Glut2)-positive cells are sparsely distributed in brain and play an important role the stimulation of glucagon secretion response to hypoglycemia. We aimed determine if Glut2-positive can influence another hypoglycemia, i.e. increased cerebral blood flow (CBF). CBF adult male mice devoid Glut2, either globally (ripglut1:glut2-/-) or nervous system only (NG2KO), their respective controls were studied under basal glycemia insulin-induced hypoglycemia using quantitative...
The ventromedial nucleus of the hypothalamus (VMN) is involved in counterregulatory response to hypoglycemia. VMN neurons activated by hypoglycemia (glucose inhibited, GI neurons) have been assumed play a critical, although untested role this response. Here, we show that expression dominant negative form AMP-activated protein kinase (AMPK) or inactivation AMPK <i>α1</i> and <i>α2</i> subunit genes Sf1 selectively suppressed neuron activity. We found...