A5034783300- Axon Guidance and Neuronal Signaling
- Regulation of Appetite and Obesity
- Receptor Mechanisms and Signaling
- Long-Term Effects of COVID-19
- Neurogenesis and neuroplasticity mechanisms
- Neuroendocrine regulation and behavior
- Olfactory and Sensory Function Studies
- Nicotinic Acetylcholine Receptors Study
- Advanced Fluorescence Microscopy Techniques
- Neurotransmitter Receptor Influence on Behavior
- Neuroscience and Neuropharmacology Research
- Cell Image Analysis Techniques
- Sleep and Wakefulness Research
- Neural dynamics and brain function
- Developmental Biology and Gene Regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Hedgehog Signaling Pathway Studies
- Genetic and Kidney Cyst Diseases
- Adipose Tissue and Metabolism
- Anesthesia and Neurotoxicity Research
- Memory and Neural Mechanisms
- SARS-CoV-2 and COVID-19 Research
- Photoreceptor and optogenetics research
- Cellular Mechanics and Interactions
- COVID-19 Clinical Research Studies
Inserm
2010-2024
Sorbonne Université
2010-2024
Institut du Cerveau
2018-2024
Centre National de la Recherche Scientifique
2010-2024
Assistance Publique – Hôpitaux de Paris
2021-2024
Pitié-Salpêtrière Hospital
2017-2022
Allen Institute for Brain Science
2020-2021
Rockefeller University
2011-2019
University of Zurich
2019
Institute of Pharmacology
2019
Although COVID-19 is considered to be primarily a respiratory disease, SARS-CoV-2 affects multiple organ systems including the central nervous system (CNS). Yet, there no consensus on consequences of CNS infections. Here, we used three independent approaches probe capacity infect brain. First, using human brain organoids, observed clear evidence infection with accompanying metabolic changes in infected and neighboring neurons. However, for type I interferon responses was detected. We...
The cerebral vasculature is a dense network of arteries, capillaries, and veins. Quantifying variations the vascular organization across individuals, brain regions, or disease models challenging. We used immunolabeling tissue clearing to image adult mouse brains developed pipeline segment terabyte-sized multichannel images from light sheet microscopy, enabling construction, analysis, visualization graphs composed over 100 million vessel segments. generated datasets 20 brains, with labeled...
Amyloidosis is a major problem in over one hundred diseases, including Alzheimer's disease (AD). Using the iDISCO visualization method involving targeted molecular labeling, tissue clearing, and light-sheet microscopy, we studied plaque formation intact AD mouse brain at up to 27 months of age. We visualized amyloid plaques 3D together with tau, microglia, vasculature. Volume imaging coupled automated detection mapping enables precise fast quantification within entire brain. The present...
Summary Although COVID-19 is considered to be primarily a respiratory disease, SARS-CoV-2 affects multiple organ systems including the central nervous system (CNS). Yet, there no consensus whether virus can infect brain, or what consequences of CNS infection are. Here, we used three independent approaches probe capacity brain. First, using human brain organoids, observed clear evidence with accompanying metabolic changes in infected and neighboring neurons. However, for type I interferon...
Gut bacteria influence brain functions and metabolism. We investigated whether this can be mediated by direct sensing of bacterial cell wall components neurons. In mice, we found that peptidoglycan plays a major role in mediating gut-brain communication via the Nod2 receptor. Peptidoglycan-derived muropeptides reach alter activity subset neurons express Nod2. Activation hypothalamic inhibitory is essential for proper appetite body temperature control, primarily females. This study identifies...
Abstract Anosmia was identified as a hallmark of COVID-19 early in the pandemic, however, with emergence variants concern, clinical profile induced by SARS-CoV-2 infection has changed, anosmia being less frequent. Here, we assessed clinical, olfactory and neuroinflammatory conditions golden hamsters infected original Wuhan strain, its isogenic ORF7-deletion mutant three variants: Gamma, Delta, Omicron/BA.1. We show that animals develop variant-dependent disease including anosmia, ORF7...
Dissecting how signaling directs axon growth During development of the nervous system, nerve cells send out projections called axons that must be guided to their proper targets. Netrins are secreted proteins bind receptors either attract or repel growing axons. Xu et al. present x-ray structures show complexes netrin with two different receptors, neogenin and DCC, have architectures. How signals remains understood in detail, but netrin's ability create assemblies probably plays a role...
Abstract Cranial lymphatic vessels (LVs) are involved in the transport of fluids, macromolecules and central nervous system (CNS) immune responses. Little information about spinal LVs is available, because these delicate structures embedded within vertebral tissues difficult to visualize using traditional histology. Here we show an extended column LV network three-dimensional imaging decalcified iDISCO + -clarified spine segments. Vertebral connect peripheral sensory sympathetic ganglia form...
Optimizing reproductive fitness in mammalians requires behavioral adaptations during pregnancy. Maternal preparatory nesting is an essential behavior for the survival of upcoming litter. Brain-wide immediate early gene mapping mice evoked by sequences revealed that phases nest construction strongly activate peptidergic neurons Edinger-Westphal nucleus pregnant mice. Genetic ablation, bidirectional neuromodulation, and vitro vivo activity recordings demonstrated these are to modulate arousal...
Abstract Tau pathology of the noradrenergic locus coeruleus (LC) is a hallmark several age-related neurodegenerative disorders, including Alzheimer’s disease. However, comprehensive neuropathological examination LC difficult due to its small size and rod-like shape. To investigate cytoarchitecture tau cytoskeletal in relation possible propagation patterns disease-associated an unprecedented large-scale three-dimensional view, we utilized volume immunostaining optical clearing technology...
In Bilateria, many axons cross the midline of central nervous system, forming well-defined commissures. Whereas in mammals functions commissures forebrain and visual system are well established, at other axial levels less clearly understood. Here, we have dissected function several hindbrain using genetic methods. By taking advantage multiple Cre transgenic lines, induced site-specific deletions Robo3 receptor. These lines developed with disruption specific sensory, motor, sensorimotor...
The left hemisphere's dominance in processing social communication has been known for over a century, but the mechanisms underlying this lateralized cortical function are poorly understood. Here, we compare structure, function, and development of each auditory cortex (ACx) mouse to look specializations that may underlie lateralization. Using Fos brain volume imaging, found greater activation ACx response vocalizations, while right responded more frequency sweeps. In vivo recordings...