A5010135765- Bioactive Compounds and Antitumor Agents
- Cancer therapeutics and mechanisms
- Immunotherapy and Immune Responses
- Synthesis and biological activity
- Antimicrobial Peptides and Activities
- Neuroinflammation and Neurodegeneration Mechanisms
- Phytochemical compounds biological activities
- Natural product bioactivities and synthesis
- Macrophage Migration Inhibitory Factor
- Piperaceae Chemical and Biological Studies
- Cancer Immunotherapy and Biomarkers
- Nuclear Receptors and Signaling
- Protein Hydrolysis and Bioactive Peptides
- Ion Channels and Receptors
- Peptidase Inhibition and Analysis
- Alzheimer's disease research and treatments
- Neurogenesis and neuroplasticity mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Renal and related cancers
- Click Chemistry and Applications
- Synthesis and Biological Evaluation
- Pluripotent Stem Cells Research
- Genomics, phytochemicals, and oxidative stress
- Immune cells in cancer
- Plant biochemistry and biosynthesis
University of California, Irvine
2019-2024
The University of Texas MD Anderson Cancer Center
2020-2023
Universidade Federal de São Paulo
2000-2020
Universidade de São Paulo
2008-2018
Alchemy (Brazil)
2018
Rockefeller University
2017
Instituto Butantan
2009-2016
Johnson University
2014
Rutgers, The State University of New Jersey
2012
Universidade de Brasília
2011
Radiotherapy (RT) of colorectal cancer (CRC) can prime adaptive immunity against tumor-associated antigen (TAA)-expressing CRC cells systemically. However, abscopal tumor remissions are extremely rare, and the postirradiation immune escape mechanisms in remain elusive. Here, we found that irradiated used ATR-mediated DNA repair signaling pathway to up-regulate both CD47 PD-L1, which through engagement SIRPα PD-1, respectively, prevented phagocytosis by antigen-presenting thereby limited TAA...
Mounting an effective immune response against cancer requires the activation of innate and adaptive cells. Metastatic melanoma is most aggressive form skin cancer. While immunotherapies have shown a remarkable success in treatment, patients develop resistance by mechanisms that include establishment suppressive tumor microenvironment. Thus, understanding how metastatic cells suppress system vital to this disease. In study, we find macrophages (MOs) dendritic (DCs) are suppressed Ig-CDR-based...
Immunotherapy using immune checkpoint inhibitors (ICIs) induces durable responses in many metastatic cancers. Metastatic uveal melanoma (mUM), typically occurring the liver, is one of most refractory tumours to ICIs and has dismal outcomes. Monosomy 3 (M3), polysomy 8q, BAP1 loss primary (pUM) are associated with poor prognoses. The presence tumour-infiltrating lymphocytes (TILs) within pUM surrounding mUM - some evidence clinical adoptive TIL transfer strongly suggests that UMs indeed...
Immune checkpoint blockade (ICB) has demonstrated an impressive outcome in patients with metastatic melanoma, yet, durable complete response; even Ipilimumab/Nivolumab combo are under 30%. Primary and acquired resistance response to ICB is commonly due a tumor immune escape mechanism dictated by the microenvironment (TME). Macrophage Migratory Inhibition Factor (MIF) emerged as immunosuppressive factor secreted TME. We have previously that of MIF-CD74 signaling on macrophages dendritic cells...
The proper functions of cortical circuits are dependent upon both appropriate neuronal subtype specification and their maturation to receive signaling. These events establish a balanced circuit that is important for learning, memory, emotion, complex motor behaviors. Recent research points mRNA metabolism as key regulator this development process. Hu antigen D (HuD), an RNA-binding protein, has been implicated in the establishment identity neurite outgrowth vitro . Therefore, we investigated...
Eugenol (EUG) is a major phenolic compound present in clove whose anti-cancer properties have been demonstrated previously. These may involves the modulation of different mechanisms, including α-estrogen receptor (αER) luminal breast cancer cells, COX-2 inhibition melanoma cells or p53 and caspase-3 activation colon cells.EUG promotes burst ROS production causing cell-cycle perturbations, mitochondria toxicity clastogenesis triggering apoptosis breast- cervix-cancer vitro.Morphological...
Brain infection by the parasite Toxoplasma gondii in mice is thought to generate vulnerability predation mechanisms that remain elusive. Monocytes play a key role host defense and inflammation are critical for controlling T. . However, dynamic regional relationship between brain-infiltrating monocytes parasites unknown. We report mobilization of inflammatory (CCR2 + Ly6C hi ) patrolling (CX3CR1 lo into blood brain during C57BL/6J CCR2 RFP/+ CX3CR1 GFP/+ mice. Longitudinal analysis using...
Abstract Rostro-caudal specificity of corticospinal tract (CST) projections from different areas the cortex was assessed by retrograde labeling with fluorogold and transfection following retro-AAV/Cre injection into spinal cord tdT reporter mice. Injections at C5 led to neurons throughout forelimb area sensorimotor a region in dorsolateral near barrel field (S2). L2 posterior (hindlimb area) but not cortex. With injections biotinylated dextran amine (BDA) main (forelimb region), labeled...
Abstract In this paper, the isolation of dillapiole ( 1 ) from Piper aduncum was reported as well semi‐synthesis two phenylpropanoid derivatives [di‐hydrodillapiole 2 ), isodillapiole 3 )], via reduction and isomerization reactions. Also, compounds' molecular properties (structural, electronic, hydrophobic, steric) were calculated investigated to establish some preliminary structure–activity relationships (SAR). Compounds evaluated for in vitro antileishmanial activity cytotoxic effects on...
Background: Pyrostegia venusta (Ker. Gawl.) Miers (Bignoniacea) is a medicinal plant from the Brazilian Cerrado used to treat leucoderma and common diseases of respiratory system. Objective: To investigate antitumor activity P.venusta extracts against melanoma. Materials Methods: The cytotoxic tumor induced cell death heptane extract (HE) P. flowers was evaluated murine melanoma B16F10-Nex2 cells in vitro syngeneic model vivo. Results: We found that HE apoptosis by disruption mitochondrial...
Capsaicin, the primary pungent component of chili pepper, has antitumor activity. Herein, we describe activity RPF151, an alkyl sulfonamide analogue capsaicin, against MDA-MB-231 breast cancer cells. RPF151 was synthetized, and molecular modeling used to compare capsaicin RPF151. Cytotoxicity on also evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5diphenyltetrazolium bromide (MTT) assay. Cell cycle analysis, flow cytometry, Western blot analysis cycle-related proteins were evaluate...
Cationic peptide with the sequence INKKI 41-45 was isolated from bovine β-casein after tryptic hydrolysis and synthetized. The aim of this work to evaluate antiproliferative activity in vitro antitumor effect animal model. cytotoxicity evaluated on B16F10 melanoma cells by MTT assay. Detection apoptosis measured using annexin V/PI double staining cell cycle analysis performed flow cytometry. Caspase-3 analyzed substrate specific fluorogenic DEVD-MCA. In vivo, tumor-bearing C57BL/6J mice....
Short peptide sequences from complementarity-determining regions (CDRs) of different immunoglobulins may exert anti-infective, immunomodulatory and antitumor activities regardless the specificity original monoclonal antibody (mAb). In this sense, they resemble early molecules innate immunity. C36L1 was identified as a bioactive light-chain CDR1 by screening 19 conserved CDR targeting murine B16F10-Nex2 melanoma. The 17-amino acid is readily taken up melanoma cells acts on microtubules...
The antitumor activity of Kielmeyera coriacea (Clusiaceae), a medicinal plant used in the treatment parasitic, as well fungal and bacterial infections by Brazilian Cerrado population, was investigated.A chloroform extract (CE) K. tested murine melanoma cell line (B16F10-Nex2) panel human tumor lines. Tumor migration determined wound-healing assay vivo CE investigated metastatic model. 1H NMR GC/MS were to determine chemical composition.We found that exhibited strong cytotoxic against cells...