A5076463216- Cervical Cancer and HPV Research
- Cholesterol and Lipid Metabolism
- T-cell and Retrovirus Studies
- Microbial infections and disease research
- Virus-based gene therapy research
- Molecular Biology Techniques and Applications
- Animal Disease Management and Epidemiology
- Cancer-related Molecular Pathways
- MicroRNA in disease regulation
- Veterinary Oncology Research
- Infectious Diseases and Mycology
- Poxvirus research and outbreaks
- Drug Transport and Resistance Mechanisms
- Vector-Borne Animal Diseases
- COVID-19 Clinical Research Studies
- DNA Repair Mechanisms
- Genomics, phytochemicals, and oxidative stress
- CRISPR and Genetic Engineering
- Peroxisome Proliferator-Activated Receptors
- Viral-associated cancers and disorders
- Epigenetics and DNA Methylation
- Cancer, Lipids, and Metabolism
- Extracellular vesicles in disease
- Cardiovascular Disease and Adiposity
- Autophagy in Disease and Therapy
Instituto Butantan
2013-2022
Universidade Federal de São Paulo
2013-2020
Universidade Federal da Integração Latino-Americana
2020
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2017-2019
Universidade de São Paulo
2013-2018
Universidade Cidade de São Paulo
2013
Universidade Federal de Pernambuco
2009-2011
Eugenol (EUG) is a major phenolic compound present in clove whose anti-cancer properties have been demonstrated previously. These may involves the modulation of different mechanisms, including α-estrogen receptor (αER) luminal breast cancer cells, COX-2 inhibition melanoma cells or p53 and caspase-3 activation colon cells.EUG promotes burst ROS production causing cell-cycle perturbations, mitochondria toxicity clastogenesis triggering apoptosis breast- cervix-cancer vitro.Morphological...
7-Ketocholesterol (7-KC) is a cholesterol oxidation product with several biological functions. 7-KC has the capacity to cause cell death depending on concentration and specific type. Mesenchymal stem cells (MSCs) are multipotent ability differentiate into various types of cells, such as osteoblasts adipocytes, among others. MSCs contribute development suitable niche for hematopoietic involved in diseases, leukemia, yet unknown extent. Here, we describe effect bone marrow from patients acute...
Bovine papillomavirus (BPV) is an oncogenic virus related to serious livestock diseases. Oncoproteins encoded by BPV are involved in several steps of cellular transformation and have been reported as presenting clastogenic effects peripheral lymphocytes primary culture cells. The aim this study was evaluate the potential types 1, 2, 4 comet assay. Peripheral blood collected from 37 bovines, 32 infected with different levels papillomatosis (12 animals no affection) five calves, free (negative...
Bovine papillomavirus (BPV) is an oncogenic virus associated with benign and malignant lesions, which result in notable economic losses. Peripheral blood samples cutaneous papillomas were obtained from four adult beef cattle. Viral molecular identification was performed using specific primers for BPV-1, -2 -4 diagnosis FAP59/FAP64 skin papillomas. Histopathologic examination done as a complementary differential diagnosis. The fragments purified, sequenced, compared BLASTn. showed the...
BPV in bovine blood and lymphocytes 89%.The detection of viral DNA whole lymphocyte cultures is evidence that this virus carried by lymphocytes.
Bovine papillomavirus (BPV) is recognized as a causal agent of benign and malignant tumors in cattle. Thirteen types BPV are currently characterized classified into three distinct genera, associated with different pathological outcomes. The described well other putative ones have been demonstrated by molecular biology methods, mainly the employment degenerated PCR primers. Specifically, divergences nucleotide sequence L1 gene useful for identification classification new types. On present...
The bovine papillomavirus (BPV) causes papillomas that regress spontaneously, but can also progress to malignancy. This study evaluated the role of BPV in oncogenesis. Twenty-four samples from uninfected calves and infected cattle were subjected molecular diagnosis, as well histopathological immunohistochemical analyses. comet assay (CA) was used evaluate clastogenic potential BPV. results confirmed presence BPV-2, 3, 5, 9 samples. Histopathological analysis revealed acanthosis,...
The bovine papillomavirus (BPV) is the etiological agent of papillomatosis, which causes significant economic losses to livestock, characterized by presence papillomas that regress spontaneously or persist and progress malignancy. Currently, there are 13 types BPVs described in literature as well 32 putative new types. This study aimed isolate viral particles BPV from skin papillomas, using a novel isolation method. virus were previously identified with primers designed. 77 cutaneous...
Bovine papillomaviruses (BPVs) are recognized as causal agents of benign and malignant tumors in cattle.Thirteen types BPVs have already been described classified into 3 distinct genera.Divergences the nucleotide sequence L1 gene used to identify new viral through employment PCR assays with degenerated primers.In present study, a method for identifying based on PCR-RFLP DNA sequencing allowed identification putative Deltapapillomavirus, designated JN/3SP (JQ280500.1).The analysis showed that...
Ten types of bovine papillomavirus (BPV) have been described and there are reports viral transmission via blood. The presence DNA in lymphocytes was to be associated with chromosome instability these cells. This study presents an evaluation short‐term peripheral lymphocyte cultures from cows presenting skin papillomatosis, compared asymptomatic infected animals non‐infected healthy bovines. In a total 2203 cells, 918 (42%) showed at least one aberration: 42.7 (±7.8) papillomatosis (BPV + W),...
Bovine papillomavirus (BPV) is an oncogenic virus with mucous and epithelial tropism. Possible productive infection in other tissues, such as blood, has been hypothesized. In order to investigate this possibility, three samples of skin papillomas blood were collected from bovines BPV five peripheral one sample normal tissue a calf without infection. Peripheral mononuclear cells (PBMCs) isolated whole examined by reverse transcription-polymerase chain reaction, immunofluorescence, situ...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been responsible for the severe pandemic of disease, disease 2019 (COVID-19), experienced in 21st century. The clinical manifestations range from mild symptoms to abnormal blood coagulation and failure. In cases, COVID-19 manifests as a thromboinflammatory disease. Damage vascular compartment caused by SARS-CoV-2 linked thrombosis, triggered an enhanced immune response. molecular mechanisms underlying endothelial activation...
THE MAJORITY OF MALIGNANT CELLS PRESENT GENETIC INSTABILITY WITH CHROMOSOME NUMBER CHANGES PLUS SEGMENTAL DEFECTS: these changes involve intact chromosomes and breakage-induced alterations. Some pathways of chromosomal instability have been proposed as random breakage, telomere fusion, centromere fission. Chromosome alterations in tumor cells described animal models vitro experiments. One important question is about possible discrepancies between models, studies, the real events cancer vivo....
Although papillary thyroid carcinoma (PTC) has a good prognosis, 20–90% of patients show metastasis to regional lymph nodes and 10–15% distant sites. Metastatic disease represents the main clinical challenge that impacts survival rate. We previously showed LIMD2 was novel metastasis-associated gene. In this study, interrogate role in cancer invasion metastasis, we used CRISPR-mediated knockout (KO) PTC cells (BCPAP TPC1). Western blot high-content screening (HCS) analysis confirmed...
Oxysterols are 27-carbon oxidation products of cholesterol metabolism. possess several biological actions, including the promotion cell death. Here, we examined ability 7-ketocholesterol (7-KC), cholestane-3β-5α-6β-triol (triol), and a mixture 5α-cholestane-3β,6β-diol 5α-cholestane-3β,6α-diol (diol) to promote death in human breast cancer line (MDA-MB-231). We determined viability, after 24-h incubation with oxysterols. These oxysterols promoted apoptosis. At least part observed effects by...
Bovine papillomavirus (BPV) is considered a useful model to study HPV oncogenic process. BPV interacts with the host chromatin, resulting in DNA damage, which attributed E5, E6, and E7 viral oncoproteins activity. However, mechanisms of E6 oncoprotein per se remain unknown. This aimed evaluate mutagenic potential Bos taurus type 1 (BPV-1) recombinant by cytokinesis-block micronucleus assay (CBMNA) comet (CA). Peripheral blood samples five calves were collected. Samples subjected molecular...
Bovine papillomaviruses (BPVs) are recognized as the causal agents of economical relevant diseases in cattle, associated with development tumors skin and mucosa. The oncogenesis process is mainly different viral oncoprotein expressions, which involved cell transformation. expression characterization recombinant oncoproteins represent an attractive strategy to obtain biotechnological products antibodies potential vaccines, Thus, aim this work was clone express BPV-1 BPV-2 E6 proteins perform...
Bovine papillomavirus (BPV) is the etiological agent of bovine papillomatosis (BP), infectious disease, characterized by presence multiples papillomas that can regress spontaneously or progress to malignances.Although recognized as mutagen, BPV action following cancer initiation remains few explored, since studies about progression and metastasis are based on cell cultures.The lack attention in vitro models a reflection replication paradigm, which dependent epithelium differentiation.Since...