A5003229897- Leptospirosis research and findings
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Venomous Animal Envenomation and Studies
- Viral Infections and Vectors
- Bacteriophages and microbial interactions
- Antimicrobial Peptides and Activities
- Respiratory viral infections research
- Pneumonia and Respiratory Infections
- Biochemical and Structural Characterization
- Veterinary medicine and infectious diseases
- Bacterial Genetics and Biotechnology
- Enterobacteriaceae and Cronobacter Research
- Salmonella and Campylobacter epidemiology
- Bacterial biofilms and quorum sensing
- Vector-borne infectious diseases
- Bacterial Infections and Vaccines
- Virus-based gene therapy research
- Urinary Tract Infections Management
- Pharmaceutical and Antibiotic Environmental Impacts
- Study of Mite Species
- Rabies epidemiology and control
- Aquaculture disease management and microbiota
- Vibrio bacteria research studies
- Viral gastroenteritis research and epidemiology
Instituto Butantan
2016-2025
Universidade de São Paulo
2006-2009
University of Vassouras
2009
Hospital Federal dos Servidores do Estado
2009
Abstract Efforts to describe toxins from the two major families of venomous snakes (Viperidae and Elapidae) usually reveal proteins belonging few structural types, particular each family. Here we carried on an effort determine uncommon cDNAs that represent possible new Lachesis muta (Viperidae). In addition nine classes typical toxins, atypical molecules never observed in hundreds Viperidae studied so far are highly expressed: a diverging C-type lectin is related but appears be independently...
The role of TlyA, TlyB and TlyC proteins in the biology Leptospira is still uncertain. Although these have been considered as putative hemolysins, we demonstrate that leptospiral recombinant do not possess hemolytic activity. However, further experiments showed a surface‐exposed protein seems to bind laminin, collagen IV fibronectin. expression both was detected vitro vivo. Our findings suggest are directly involved hemolysis, may contribute binding extracellular matrix (ECM) during host infection.
A new antimicrobial peptide, herein named Stigmurin, was selected based on a transcriptomic analysis of the Brazilian yellow scorpion Tityus stigmurus venom gland, an underexplored source for toxic peptides with possible biotechnological applications. Stigmurin investigated in silico, by circular dichroism (CD) spectroscopy, and vitro. The CD spectra suggested that this peptide interacts membranes, changing its conformation presence amphipathic environment, predominance random coil...
Scorpion venom constitutes a rich source of biologically active compounds with high potential for therapeutic and biotechnological applications that can be used as prototypes the design new drugs. The aim this study was to characterize structural conformation, evaluate antimicrobial activity, gain insight into possible action mechanism underlying it, two analog peptides scorpion peptide Stigmurin, named StigA25 StigA31. amino acid substitutions in native sequence lysine residues resulted...
Scorpion venom is a rich source of biologically active components and various peptides with high-potential therapeutic use that have been characterized for their antimicrobial antiproliferative activities. Stigmurin peptide identified from the Tityus stigmurus gland high antibacterial activities low toxicity. Amino acid substitutions in without disulfide bridge sequence made aim reducing toxicity increasing biological The purpose this study was to evaluate structural conformation stability,...
Hybrid strains of Escherichia coli combine virulence traits diarrheagenic (DEC) and extraintestinal pathogenic E. (ExPEC), but it is poorly understood whether these combined features improve the potential such strains. We have previously identified a uropathogenic (UPEC) strain (UPEC 252) harboring eae gene, located in locus enterocyte effacement (LEE) pathogenicity island, that encodes adhesin intimin. The LEE-encoded proteins allow enteropathogenic (EPEC) enterohemorrhagic (EHEC) to form...
The role of innate immune response in protection against leptospirosis is poorly understood. We examined the expression chemokine CXCL2/MIP-2 and cytokine TNF-α experimental resistant susceptible mice models, C3H/HeJ, C3H/HePas BALB/c strains, using a virulent strain Leptospira interrogans serovar Copenhageni. Animals were infected intraperitoneally with 10(7) cells development disease was followed. Mortality C3H/HeJ observed whereas presented jaundice remained asymptomatic. infection...
Although native to the tropical and subtropical areas of Southeast Asia, Aedes albopictus is now found on five continents, primarily due its great capacity adapt different environments. This species considered a secondary vector dengue virus in several countries. Wing geometric morphometrics widely used furnish morphological markers for characterisation identification medical importance assessment population dynamics. In this work, we investigated metric differentiation wings Ae. samples...
The presence of bioactive peptides in animal venoms has been targeted scientific research for assessing biological activities, as well mechanisms action. A recent study by our group observed hypotensive action TistH (Tityus stigmurus Hypotensin), a peptide deduced from the transcriptome T. venom gland. present aims to analyze structure properties and evaluate its toxicity on normal tumor cells, vitro antimicrobial activity, inflammatory effect. Circular dichroism analyses showed general...
Microbial resistance to conventional antibiotics is a public health problem worldwide, motivating the search for new therapeutic alternatives in varied natural sources. Cationic peptides without disulfide bridges from scorpions have been targeted this context, mainly due their multifunctional action and limited ability of microorganisms develop against them. The present study was focused on Stigmurin TsAP-2, cationic found transcriptome venom gland scorpion Tityus stigmurus. aims were:...
Febrile neutropenia (FN) is one of the most common complications in pediatric oncology patients. It has a complex etiologic nature, which majority cases remains unclear. Intervention often follows empirical treatment protocols, mainly using broad-spectrum antibiotics. To evaluate potential viral agents, this study applied metagenomics to paired plasma and oropharyngeal samples obtained from patients with oncological diseases diagnosed FN. Metagenomic sequencing was performed on 15 FN at...
Viral metagenomics has expanded significantly in recent years due to advancements next-generation sequencing, establishing it as the leading method for identifying emerging viruses. A crucial step is taxonomic classification, where sequence data assigned specific taxa, thereby enabling characterization of species composition within a sample. Various classifiers have been developed years, each employing distinct classification approaches that produce varying results and abundance profiles,...
ABSTRACT Despite the substantial beneficial effects of incorporating 7-valent pneumococcal conjugate vaccine (PCV7) into immunization programs, serotype replacement has been observed after its widespread use. As there are many serotypes currently documented, use a relying on protective proteins as active carriers is promising alternative to expand PCV coverage. In this study, capsular polysaccharide 6B (PS6B) and recombinant surface protein A (rPspA), well-known antigen from Streptococcus...
Viral metagenomics is increasingly being used for the identification of emerging and re-emerging viral pathogens in clinical samples with unknown etiology. The objective this study was to shield light on metavirome composition obtained from patients history compatible an arboviral infection, but that presented inconclusive results when tested using RT-qPCR. amplification might be indication presence agent inefficiently amplified by conventional PCR techniques. A total eight serum routinely...
Background: Since its first report in the country 2013, NDM-producing Enterobacterales have been identified all Brazilian administrative regions. In this study, we characterized by antimicrobial susceptibility testing and molecular typing a large collection of Klebsiella isolates from different hospitals Brazil, mainly state Sao Paulo, over last decade. Methods: Bacterial positive for blaNDM-genes were MALDI-TOF MS submitted to disk diffusion or broth microdilution (for polymyxin B). All...
Until 2015, polymyxin resistance was primarily attributed to chromosomal mutations. However, with the first report of mobile colistin (mcr-1) in commensal Escherichia coli from food animals China, landscape has changed. To evaluate presence Salmonella spp., a drop screening test for and B carried out on 1156 isolates non-human origin (animals, food, environment), received Brazil, between 2016 2021. Subsequently, 210 resistant results were subjected gold-standard (broth microdilution) both B....