A5049891642- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- Venomous Animal Envenomation and Studies
- Corneal Surgery and Treatments
- Genetic Neurodegenerative Diseases
- Muscle Physiology and Disorders
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Antimicrobial Peptides and Activities
- Neurogenesis and neuroplasticity mechanisms
- Reproductive Biology and Fertility
- Biochemical and Structural Characterization
- Nerve injury and regeneration
- Renal and related cancers
- Ocular Surface and Contact Lens
- MicroRNA in disease regulation
- Biomedical Ethics and Regulation
- Extracellular vesicles in disease
- 3D Printing in Biomedical Research
- Advanced biosensing and bioanalysis techniques
- Mycobacterium research and diagnosis
- Corneal surgery and disorders
- dental development and anomalies
Instituto Butantan
2015-2025
Universidade Federal de São Paulo
2007-2024
Hospital Universitário da Universidade de São Paulo
2017
Universidade de São Paulo
2002-2014
Morpho (United States)
2011
Centro de Epilepsia y Neurocirugía Funcional
2008
Centro de Reprodução Humana Prof. Franco Junior
2006-2007
Institute of Cytology and Genetics
1991-1992
Novosibirsk State University
1992
We report the isolation of a population immature dental pulp stem cells (IDPSC), which express embryonic cell markers Oct-4, Nanog, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81 as well several other mesenchymal during at least 25 passages while maintaining normal karyotype rate expansion characteristic cells. The expression these was maintained in subclones obtained from Moreover, vitrothese can be induced to undergo uniform differentiation into smooth skeletal muscles, neurons, cartilage, bone...
Purpose.: To determine the outcome of use a tissue-engineered cell sheet composed human undifferentiated immature dental pulp stem cells (hIDPSC) for ocular surface reconstruction in an animal model total limbal deficiency (LSCD). Methods.: LSCD was induced by application 0.5 M NaOH to right eye rabbits 25 seconds (mild chemical burn [MCB]) and 45 (severe [SCB]). After 1 month, superficial keratectomy performed remove fibrovascular pannus that covered animals' burned corneas. A hIDPSC...
The main aim of this study is to evaluate the capacity human dental pulp stem cells (hDPSC), isolated from deciduous teeth, reconstruct large-sized cranial bone defects in nonimmunosuppressed (NIS) rats. To our knowledge, these were not used before similar experiments. We performed two symmetric full-thickness (5 × 8 mm) on each parietal region eight NIS In six them, left side was supplied with collagen membrane only and right (RS) hDPSC. rats, RS had nothing added at (controls). Cells after...
Abstract Background The golden retriever muscular dystrophy (GRMD) dogs represent the best available animal model for therapeutic trials aiming at future treatment of human Duchenne (DMD). We have obtained a rare litter six GRMD (3 males and 3 females) born from an affected male carrier female which were submitted to trial with adult stem cells investigate their capacity engraft into muscles by local as compared systemic injection without any immunosuppression. Methods Human Immature Dental...
Dental pulp (DP) can be extracted from child's primary teeth (deciduous), whose loss occurs spontaneously by about 5 to 12 years. Thus, DP presents an easy accessible source of stem cells without ethical concerns. Substantial quantities excellent quality and at early (2–5) passages are necessary for clinical use, which currently is a problem use adult cells. Herein, DPs were cultured generating least during six months through multiple mechanical transfers into new culture dish every 3–4...
Limbal stem cells (LSC) are self-renewing, highly proliferative in vitro, which express a set of specific markers and vivo have the capacity to reconstruct entire corneal epithelium cases ocular surface injury. Currently, LSC transplantation is commonly used procedure patients with either uni- or bilateral total limbal deficiency (TLSCD). Although holds great promise for patients, several problems need be overcome. In order find an alternative source that can partially substitute cornea...
Recently we have shown that crotamine, a toxin from the South American rattlesnake Crotalus durissus terrificus venom, belongs to family of cell-penetrating peptides. Moreover, crotamine was demonstrated be marker centrioles, cell cycle, and actively proliferating cells. Herein show this at non-toxic concentrations is also capable binding electrostatically plasmid DNA forming DNA-peptide complexes whose stabilities overcome need for chemical conjugation carrying nucleic acids into...
Induced pluripotent stem cells (iPSCs) can be created by forcing expression of certain genes in fibroblasts or other somatic cell types, reversing them to a state similar that embryonic (ESC). Here, we used human immature dental pulp (hIDPSCs) as an alternative source for creating iPSC. hIDPSCs easily isolated from accessible tissue young and adult patients. possess fibroblast-like morphology, retaining characteristics multipotent cells. Reprogramming was fast, producing primary hIDPSC-iPSC...
During fetal development, mesenchymal progenitor (MP) cells are co-localized in major hematopoietic territories, such as yolk sac (YS), bone marrow (BM), liver (LV), and others. Studies using mouse human MP isolated from fetus have shown that these very similar but not identical to adult stem (MSC). Their differentiation potential is usually restricted production of highly committed osteogenic chondrogenic precursors. Such properties can be useful for tissue regeneration, when a great number...
Strategies aimed at improving spinal cord regeneration after trauma are still challenging neurologists and neuroscientists throughout the world. Many cell-based therapies have been tested, with limited success in terms of functional outcome. In this study, we investigated effects human dental pulp cells (HDPCs) a mouse model compressive injury (SCI). These present some advantages, such as ease extraction process, expression trophic factors embryonic markers from both ecto-mesenchymal...
Cleft lip and palate (CLP), one of the most frequent congenital malformations, affects alveolar bone in great majority cases, reconstruction this defect still represents a challenge rehabilitation these patients. One current promising strategy to achieve goal is use marrow stem cells (BMSC); however, isolation BMSC or iliac bone, which mostly used graft surgical repair patients, confers site morbidity donor. Therefore, order identify new alternative source with osteogenic potential without...
Abstract: In order to increase the amount of available bone where dental implants must be placed, present study has associated platelet‐rich plasma (PRP) and mononuclear cells (MNCs) from marrow aspirate scaffold (BS) in 32 patients aged between 45 75 years old. The MNC attainment adherence BS were confirmed through histology, cell culture, scanning electron microscopy. clinical results, analyzed by computed tomography, have showed that scaffolds well integrated adapted cortical bone. We can...
In horses, stem cell therapies are a promising tool to the treatment of many injuries, which common consequences athletic endeavor, resulting in high morbidity and often compromising performance. spite advantages, isolation cells similar human, from equine adipose tissue, occurred only recently. The aim this study was isolate tissue-derived progenitor (eAT-PC), characterize their proliferative potential, differentiation capacity before after cryopreservation. cells, isolated horse presented...
Selective anticancer cell activity for both cell-penetrating and cationic antimicrobial peptides has previously been reported. As crotamine possesses activities similar to of these, this study investigates crotamine's toxicity in vitro vivo.In cancer viability was evaluated after treatment with 1 5 μg/ml crotamine. In vivo cytotoxic effects C57Bl/6J mice bearing B16-F10 primary cutaneous melanoma were tested, two groups each containing 35 mice. The crotamine-treated group received μg/day per...
Keratoconjunctivitis sicca (KCS) is a dysfunction in tear production associated with clinical signs, which include conjunctival hyperemia, ocular discharge, discomfort, pain, and, eventually, corneal vascularization and pigmentation. Immunosuppressive drugs are routinely administrated for long periods to treat KCS but side effects limited results. Evaluation of the benefits intralacrimal transplantation allogeneic mesenchymal stem cells (MSCs) dogs mild-moderate severe was done. A total 24...
Biphasic calcium phosphate (BCP) bioceramics have been successfully applied in a broad variety of presentation forms and with different ratios hydroxyapatite (HA) β-tricalcium (β-TCP). BCPs loaded stem cells from origins for bone tissue engineering purposes, but evidence cell behavior on compositions (various HA/β-TCP ratios) physical features is limited. We compared the adhesion, proliferation, viability osteogenic potential human mesenchymal (MSCs) granular equal ratio diverse particle...
PURPOSE: Evaluate the bone tissue recovery following transplantation of ovine mesenchymal stem cells (MSC) from marrow and human immature dental-pulp (hIDPSC) in model induced osteonecrosis femoral head (ONFH). METHODS: Eight sheep were divided three experimental groups. First group was composed by four animals with ONFH ethanol through central decompression (CD), for control without any treatment. The second third compose two animals, six weeks after induction received heterologous MSC (CD...
Mesenchymal stem cells (MSCs) due to their self-renewal potential and differentiation capacity are useful for tissue regeneration. Immunomodulatory trophic properties of MSCs were demonstrated suggesting use as medicinal signaling able positively change local environment in injured tissue. Equine endometrosis is a progressive degenerative disease responsible glandular alterations endometrial fibrosis which causes infertility mares. More precisely, this characterized by phenotypic changes the...