Rishika Prasad
A5020134508- Advanced Biosensing Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Cytomegalovirus and herpesvirus research
- Mycobacterium research and diagnosis
- Cancer Immunotherapy and Biomarkers
- Neutropenia and Cancer Infections
- Immunotherapy and Immune Responses
- Gut microbiota and health
- Single-cell and spatial transcriptomics
- Hematopoietic Stem Cell Transplantation
- CAR-T cell therapy research
- Cytokine Signaling Pathways and Interactions
- Phagocytosis and Immune Regulation
- interferon and immune responses
- T-cell and B-cell Immunology
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Genetic factors in colorectal cancer
- Software System Performance and Reliability
- Glioma Diagnosis and Treatment
- Nanocomposite Films for Food Packaging
- Antibiotics Pharmacokinetics and Efficacy
- Metabolism, Diabetes, and Cancer
- Clostridium difficile and Clostridium perfringens research
- PI3K/AKT/mTOR signaling in cancer
The University of Texas MD Anderson Cancer Center
2019-2024
The University of Texas Health Science Center at Houston
2021-2022
Defence Research Laboratory
2016
University of Westminster
2015
Radiotherapy (RT) of colorectal cancer (CRC) can prime adaptive immunity against tumor-associated antigen (TAA)-expressing CRC cells systemically. However, abscopal tumor remissions are extremely rare, and the postirradiation immune escape mechanisms in remain elusive. Here, we found that irradiated used ATR-mediated DNA repair signaling pathway to up-regulate both CD47 PD-L1, which through engagement SIRPα PD-1, respectively, prevented phagocytosis by antigen-presenting thereby limited TAA...
The purpose of this study is to determine if inhibition mitochondrial oxidative phosphorylation (OxPhos) an effective strategy against MAPK pathway inhibitor (MAPKi)-resistant BRAF-mutant melanomas.Experimental Design: antimelanoma activity IACS-010759 (OPi), a novel OxPhos complex I inhibitor, was evaluated in vitro and vivo. Mechanistic studies predictors response were using molecularly metabolically stratified melanoma cell lines. 13C-labeling targeted metabolomics used evaluate the...
Intratumoral injection of cyclic dinucleotide (CDN) agonists the stimulator interferon genes (STING) pathway engages innate immune activation and priming adaptive effectors to foster local distal tumor clearance. Despite proven therapeutic efficacy in preclinical models, a thorough understanding how CDNs reprogram suppressive myeloid stroma mouse man is lacking.Here, we perform deep transcript-level protein-level profiling myeloid-derived suppressor cells M2 macrophages following stimulation...
Not all patients with cancer and severe neutropenia develop fever, the fecal microbiome may play a role. In single-center study of undergoing hematopoietic cell transplant ( n = 119), was characterized at onset neutropenia. A total 63 (53%) developed subsequent their displayed increased relative abundances Akkermansia muciniphila , species mucin-degrading bacteria P 0.006, corrected for multiple comparisons). Two therapies that induce neutropenia, irradiation melphalan, similarly expanded A....
Identifying bioenergetics that facilitate the epithelial to mesenchymal transition (EMT) in breast cancer cells may uncover targets treat incurable metastatic disease. Metastasis is number one cause of cancer-related deaths; therefore, it urgent identify new treatment strategies prevent initiation metastasis. To characterize EMT, we compared metabolic activities and gene expression induced differentiate into state with their counterparts. We found levels GLS2, which encodes a glutaminase,...
Abstract Background The lack of murine glioblastoma models that mimic the immunobiology human disease has impeded basic and translational immunology research. We, therefore, developed stem cell lines derived from Nestin-CreERT2QkL/L; Trp53L/L; PtenL/L (QPP) mice driven by clinically relevant genetic mutations common in glioblastoma. This study aims to determine immune sensitivities these QPP immunocompetent hosts their underlying mechanisms. Methods differential responsiveness was assessed...
Acute gastrointestinal intestinal GVHD (aGI-GVHD) is a serious complication of allogeneic hematopoietic stem cell transplantation, and the microbiota known to impact on its severity. However, an association between treatment response aGI-GVHD has not been well-studied. In cohort patients with (n=37), we found that non-response standard therapy corticosteroids was associated prior carbapenem antibiotics loss
Abstract Tools for genome-wide rapid identification of peptide–major histocompatibility complex targets T-cell receptors (TCR) are not yet universally available. We present a new antigen screening method, the T-synapse (Tsyn) reporter system, which includes antigen-presenting cells (APC) with Fas-inducible NF-κB and T nuclear factor activated (NFAT) reporter. To functionally screen target antigens from cDNA library, productively interacting cell–APC aggregates were detected by dual-reporter...
<div>Abstract<p>Tools for genome-wide rapid identification of peptide–major histocompatibility complex targets T-cell receptors (TCR) are not yet universally available. We present a new antigen screening method, the T-synapse (Tsyn) reporter system, which includes antigen-presenting cells (APC) with Fas-inducible NF-κB and T nuclear factor activated (NFAT) reporter. To functionally screen target antigens from cDNA library, productively interacting cell–APC aggregates were...
<p>Tsyn-seq system spike-in assay.</p>
<p>Activation of Fas-iNFκB reporter in FIR-APCs.</p>
<p>Supplementary Data</p>
<p>Supplementary Data</p>
<p>Aggregates of interacting target cells and T cells.</p>
<p>Aggregates of interacting target cells and T cells.</p>
<p>Generation of reporter cells overexpressing CaSki cDNA library.</p>
<p>Tsyn-seq system spike-in assay.</p>
<div>Abstract<p>Tools for genome-wide rapid identification of peptide–major histocompatibility complex targets T-cell receptors (TCR) are not yet universally available. We present a new antigen screening method, the T-synapse (Tsyn) reporter system, which includes antigen-presenting cells (APC) with Fas-inducible NF-κB and T nuclear factor activated (NFAT) reporter. To functionally screen target antigens from cDNA library, productively interacting cell–APC aggregates were...
<p>Generation of reporter cells overexpressing CaSki cDNA library.</p>