A5056515397- Neuroscience of respiration and sleep
- Neuroendocrine regulation and behavior
- Neonatal Respiratory Health Research
- Sleep and Wakefulness Research
- Infant Health and Development
- Neuroinflammation and Neurodegeneration Mechanisms
- Zebrafish Biomedical Research Applications
- Respiratory Support and Mechanisms
- Vestibular and auditory disorders
- Neonatal and fetal brain pathology
- Heart Rate Variability and Autonomic Control
- Neurogenesis and neuroplasticity mechanisms
- Barrier Structure and Function Studies
- Congenital heart defects research
- Cerebral Venous Sinus Thrombosis
- Spinal Cord Injury Research
- Neurobiology and Insect Physiology Research
- Ocular Surface and Contact Lens
- Photoreceptor and optogenetics research
- Dysphagia Assessment and Management
- Tracheal and airway disorders
- Neurological Disease Mechanisms and Treatments
- Circadian rhythm and melatonin
- Neuroscience and Neuropharmacology Research
- Infant Development and Preterm Care
Centre National de la Recherche Scientifique
2016-2025
Université de Bordeaux
2016-2025
Institut de Neurosciences Cognitives et Intégratives d’Aquitaine
2015-2025
École Pratique des Hautes Études
2021-2022
Laboratoire de Neurobiologie Cellulaire et Moléculaire
2003-2012
Génétique Animale et Biologie Intégrative
2003-2012
Centre de Génétique Moléculaire
2010
Institut National de la Recherche Agronomique
2010
Université Paris-Sud
2010
University of Chicago
2000-2001
Glutamatergic excitatory neurotransmission is dependent on glutamate release from presynaptic vesicles loaded by three members of the solute carrier family, Slc17a6–8, which function as vesicular transporters (VGLUTs). Here, we show that VGLUT2 (Slc17a6) required for life ex utero . Vglut2 null mutant mice die immediately after birth because absence respiratory behavior. Investigations at embryonic stages revealed neural circuits in location pre-Bötzinger (PBC) inspiratory rhythm generator...
Breathing is a spontaneous, rhythmic motor behavior critical for maintaining O 2 , CO and pH homeostasis. In mammals, it generated by neuronal network in the lower brainstem, respiratory rhythm generator (Feldman et al., 2003). A century-old tenet physiology posits that chemoreflex, stimulation of breathing an increase partial pressure blood, indispensable breathing. Here we have revisited this postulate with help mouse genetics. We engineered conditional mutant which toxic PHOX2B 27Ala...
In the respiratory network of mice, we characterized with whole cell patch-clamp technique pacemaker properties in neurons discharging phase inspiration. The was isolated a transverse brain stem slice containing pre-Bötzinger complex (PBC), presumed site for rhythm generation. After blockade activity 6-cyano-7-nitroquinoxalene-2,3-dione (CNQX), 18 52 inspiratory exhibited endogenous activity, which voltage dependent, could be reset by brief current injections and entrained repetitive...
We examined the role of hyperpolarization-activated current ( I h ) in generation respiratory rhythm using a spontaneously active brainstem slice mice. This preparation contains hypoglossus (XII) nucleus, which is activated in-phase with inspiration and pre-Bötzinger complex (PBC), presumed site for generation. Voltage-clamp recordings n = 90) indicate that cesium (Cs) (5 m blocked 77.2% current, ZD 7288 (100 μ 85.8% current. blockade increased frequency by 161% Cs 150% amplitude integrated...
The retrotrapezoid nucleus (RTN) is a group of neurons in the rostral medulla, defined here as Phox2b-, Vglut2-, neurokinin1 receptor-, and Atoh1-expressing cells parafacial region, which have been proposed to function both generators respiratory rhythm central chemoreceptors. present study was undertaken assess these two putative functions using genetic tools. We generated conditional Phox2b mutations, target different subsets Phox2b-expressing cells, but common massive depletion RTN...
Neurotransmitter glutamate has been thought to derive mainly from glutamine via the action of glutaminase type 1 (GLS1). To address importance this pathway in glutamatergic transmission, we knocked out GLS1 mice. The insertion a STOP cassette by homologous recombination produced null allele that blocked transcription, encoded no immunoreactive protein, and abolished enzymatic activity. Null mutants were slightly smaller, deficient goal-directed behavior, hypoventilated, died first postnatal...
To obtain insights into the emergence of rhythmogenic circuits supporting respiration, we monitored spontaneous activities in isolated brainstem and medullary transverse slice preparations mouse embryos, combining electrophysiological calcium imaging techniques. At embryonic day 15 (E15), a restricted region ventral to nucleus ambiguus, observed onset sustained high-frequency (HF) respiratory-like activity addition preexisting low-frequency having distinct initiation site, spatial extension,...
Many networks generate distinct rhythms with multiple frequency and amplitude characteristics. The respiratory network in the pre-Bötzinger complex (pre-Böt) generates both low-frequency, large-amplitude sigh rhythm a faster, smaller-amplitude eupneic rhythm. Could same set of pacemakers rhythms? Here we used an vitro brainslice preparation. We describe subset synaptically isolated that spontaneously two bursting patterns. These patterns resemble activity including sigh-like bursts occur at...
Neuromodulatory inputs play a critical role in governing the expression of rhythmic motor output by pyloric network crustacean stomatogastric ganglion (STG). When these are removed cutting primarily afferent nerve (stn) to STG, neurons rapidly lose their ability burst spontaneously, and falls silent. By using extracellular recordings from long-term organotypic preparations nervous system lobster Jasus lalandii, we investigating whether modulatory exert regulatory influences on operation...
Genetic loss of brain-derived neurotrophic factor (BDNF) severely disrupts brainstem control respiratory rhythmogenesis in newborn mice; however, the sites at which BDNF acts to regulate are unknown. Using immunochemical and multiplex RT-PCR analysis mouse slices, we report that receptor, Tyrosine kinase B (TrkB), is strongly expressed pre-Bötzinger complex (PBC), presumed site for rhythm generation, colocalizes with neurokinin 1 (NK1), a marker neurons critical breathing. The period...
Microglia exhibit diverse morphologies reflecting environmental conditions, maturity or functional states. Thus, morphological characterization provides important information to understand microglial roles and functions. Most recent analysis relies on classifying cells based parameters. However, this classification may lack biological relevance, as represent a continuum rather than distinct, separate groups, do not correspond mathematically defined, clusters irrelevant of function. Instead,...
In severe hypoxia the breathing frequency is modulated in a biphasic manner: an initial increase (augmentation) followed by depression and cessation of (apnea). Using mouse slice preparation that contains functional respiratory network, we aimed at identifying neurons responsible for this modulation. Whole-cell patch recordings revealed expiratory become tonically active during anoxia, indicating these cannot be Inspiratory tended to depolarize (by 6.9 mV; n = 9), rhythmic activity was...
Neonatal breathing in mammals involves multiple neuronal circuits, but its genetic basis remains unclear. Mice deficient for the zinc finger protein Teashirt 3 (TSHZ3) fail to breathe and die at birth. <i>Tshz3</i> is expressed areas of brainstem involved respiration, including pre-Bötzinger complex (preBötC), embryonic parafacial respiratory group (e-pF), cranial motoneurons that control upper airways. inactivation led pronounced cell death nucleus ambiguus induced strong alterations...
During exercise and locomotion, breathing rate rapidly increases to meet the suddenly enhanced oxygen demand. The extent which direct central interactions between spinal networks controlling locomotion brainstem are involved in this rhythm modulation remains unknown. Here, we show that isolated neonatal rat brainstem-spinal cord preparations, increase respiratory observed during fictive is associated with an excitability of pre-inspiratory neurons parafacial group (pFRG/Pre-I). In addition,...
Rhythm generation by the pyloric motor network in stomatogastric ganglion (STG) of spiny lobster requires permissive neuromodulatory inputs from other central ganglia. When these to STG are suppressed cutting single, mainly afferent nerve (stn), neurons cease burst and falls silent. However, as shown previously, if such a decentralized quiescent is maintained organ culture, rhythmicity returns after 3–4 days and, although slower, similar pattern expressed when stn intact. Here we use...
In mammals, eupnoeic breathing is periodically interrupted by spontaneous augmented breaths (sighs) that include a larger-amplitude inspiratory effort, typically followed post-sigh apnoea. Previous in vitro studies newborn rodents have demonstrated the respiratory oscillator of pre-Bötzinger complex (preBötC) can generate distinct motor patterns for both eupnoea- and sigh-related behaviour. During mouse embryonic development, preBötC begins to rhythmicity at day (E) 15.5, but network's...
Abstract Neural networks control complex motor outputs by generating several rhythmic neuronal activities, often with different time scales. One example of such a network is the pre-Bötzinger respiratory (preBötC) that can simultaneously generate fast, small-amplitude, monophasic eupneic breaths together slow, high-amplitude, biphasic augmented (sighs). However, underlying rhythmogenic mechanisms for this bimodal discharge pattern remain unclear, leaving two possible explanations: existence...
Breathing is a vital rhythmic behavior generated by hindbrain neuronal circuitry, including the preBötzinger complex network (preBötC) that controls inspiration. The emergence of preBötC activity during prenatal development has been described, but little known regarding inspiratory neurons expressing pacemaker properties at embryonic stages. Here, we combined calcium imaging and electrophysiological recordings in mouse embryo brainstem slices together with computational modeling to reveal...
Over the last two decades, nanotechnologies and use of nanoparticles represent one greatest technological advances in many fields human activity. Particles titanium dioxide (TiO2) are nanomaterials most frequently found everyday consumer products. But, due particular to their extremely small size, TiO2 (NPs) prone cross biological barriers potentially lead adverse health effects. The presence NPs placentae infant meconium has indicated unequivocally capacity for a materno-fetal transfer this...