A5046429548- Neuroinflammation and Neurodegeneration Mechanisms
- Barrier Structure and Function Studies
- Neurological Disease Mechanisms and Treatments
- Nanoplatforms for cancer theranostics
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Dementia and Cognitive Impairment Research
- Alzheimer's disease research and treatments
- Photoacoustic and Ultrasonic Imaging
- Cerebrovascular and Carotid Artery Diseases
- Cell Image Analysis Techniques
- Bioinformatics and Genomic Networks
- Health, Environment, Cognitive Aging
- Luminescence and Fluorescent Materials
- Nonlinear Optical Materials Studies
- Acute Ischemic Stroke Management
- Medical Imaging Techniques and Applications
- Cerebrovascular and genetic disorders
- Axon Guidance and Neuronal Signaling
- Intracerebral and Subarachnoid Hemorrhage Research
- Immune cells in cancer
- Long-Term Effects of COVID-19
- Nanoparticle-Based Drug Delivery
- Advanced Proteomics Techniques and Applications
- Radiomics and Machine Learning in Medical Imaging
Université Claude Bernard Lyon 1
2016-2025
Inserm
2016-2025
Lund University
2023-2024
Institut NeuroMyoGène
2019-2024
University of Geneva
2023
Centre National de la Recherche Scientifique
2016-2022
Canadian Nautical Research Society
2021
Centre de Recherche en Neurosciences de Lyon
2016
Abstract Proteomics can shed light on the dynamic and multifaceted alterations in neurodegenerative disorders like Alzheimer’s disease (AD). Combining radioligands measuring β-amyloid (Aβ) plaques tau tangles with cerebrospinal fluid proteomics, we uncover molecular events mirroring different stages of AD pathology living humans. We found 127 differentially abundant proteins (DAPs) across spectrum. The strongest Aβ-related were mainly expressed glial cells included SMOC1 ITGAM. A dozen...
Abstract INTRODUCTION Tau and neurodegeneration strongly correlate with cognitive impairment, as compared to amyloid. However, their contribution in explaining cognition predicting decline memory clinics remains unclarified. METHODS We included 94 participants Mini‐Mental State Examination (MMSE), tau positron emission tomography (PET), amyloid PET, fluorodeoxyglucose (FDG) MRI scans from Geneva Memory Center. Linear regression mediation analyses tested the independent combined association...
Abstract Microglia, the brain-resident immune cells, are highly ramified with dynamic processes transiently contacting synapses. These contacts have been reported to be activity-dependent, but this has not thoroughly studied yet, especially in physiological conditions. Here we investigate neuron-microglia and microglia morphodynamics mice an activity-dependent context such as vigilance states. We report that microglial microglia-spine regulated by spontaneous evoked neuronal activity. also...
Microglia exhibit diverse morphologies reflecting environmental conditions, maturity or functional states. Thus, morphological characterization provides important information to understand microglial roles and functions. Most recent analysis relies on classifying cells based parameters. However, this classification may lack biological relevance, as represent a continuum rather than distinct, separate groups, do not correspond mathematically defined, clusters irrelevant of function. Instead,...
The purpose of this study is to propose and validate a preclinical in vivo magnetic resonance imaging (MRI) tool monitor neuroinflammation following ischemic stroke, based on injection novel multimodal nanoprobe, NanoGd, specifically designed for internalization by phagocytic cells. First, it verified that NanoGd efficiently internalized microglia vitro. In MRI coupled with intravenous permanent middle cerebral artery occlusion mouse model results hypointense signals the lesion. these mice,...
ABSTRACT Myelination leads to the formation of myelin sheaths surrounding neuronal axons and is crucial for function, plasticity repair central nervous system (CNS). It relies on interaction oligodendrocytes: glial cells producing CNS myelin. Here, we have investigated role a component Sonic hedgehog (Shh) signalling pathway, co-receptor Boc, in developmental repairing myelination. During development, Boc mutant mice display transient decrease oligodendroglial cell density together with...
Microglia, the resident immune cells of brain, are highly ramified and motile their morphology is strongly linked to function. Microglia constantly monitor brain parenchyma crucial for maintaining homeostasis fine-tuning neuronal networks. Besides affecting neurons, anesthetics may have wide-ranging effects mediated by non-neuronal in particular microglia. We thus examined effect two commonly used anesthetic agents, ketamine/xylazine barbiturates, on microglial motility morphology. A...
Herein, we demonstrate the potential of ultrabright fluorescent silica-coated organic nanocrystals for two-photon in vivo imaging. These unique nanoparticles (NPs) containing a crystalline core small push–pull dipolar dye are specifically designed to exhibit absorption fluorescence The NPs can be easily functionalized using click chemistry pure water, with preservation core. A novel small-volume DLS technique was used evaluate effect PEGylation on colloidal stability complex media salts and...
Hybrid nanoprobe specifically designed to use as a contrast agent targeting phagocytic cells in an animal model of ischemic stroke and imaging using MRI, intravital two-photon microscopy phase X-rays imaging.
The pathophysiology underlying various manifestations of cerebral small vessel disease (cSVD) remains obscure. Using cerebrospinal fluid proximity extension assays and co-expression network analysis 2,943 proteins, we found common distinct proteomic signatures between white matter lesions (WML), microbleeds infarcts measured in 856 living patients, validated WML-associated proteins three additional datasets. Proteins indicative extracellular matrix dysregulation vascular remodeling,...
Microglia exhibit diverse morphologies reflecting environmental conditions, maturity or functional states. Thus, morphological characterization provides important information to understand microglial roles and functions. Most recent analysis relies on classifying cells based parameters. However, this classification may lack biological relevance, as represent a continuum rather than distinct, separate groups, do not correspond mathematically defined, clusters irrelevant of function. Instead,...
Microglia exhibit diverse morphologies reflecting environmental conditions, maturity or functional states. Thus, morphological characterization provides important information to understand microglial roles and functions. Most recent analysis relies on classifying cells based parameters. However, this classification is not always biologically relevant, as constitute a continuum rather than segregated groups. Instead, we propose new open-source tool, MorphoCellSorter, which assesses morphology...
Microglia exhibit diverse morphologies reflecting environmental conditions, maturity or functional states. Thus, morphological characterization provides important information to understand microglial roles and functions. Most recent analysis relies on classifying cells based parameters. However, this classification is not always biologically relevant, as constitute a continuum rather than segregated groups. Instead, we propose new open-source tool, MorphoCellSorter, which assesses morphology...
Abstract Background Cerebrospinal fluid (CSF) proteomics allows for characterization of multiple disease‐related biological processes in vivo . These likely occur along temporal cascades mirroring disease evolution. This study describes interindividual variation these cascades, the context Alzheimer’s disease. Method Participants were recruited from Swedish BioFINDER‐2 study, diagnosed as cognitively normal (n=251; CN), subjective cognitive decline (SCD; n=129), mild impairment (MCI; n=134)...
Abstract Background Substantial variability in tau accumulation patterns Alzheimer’s disease (AD) population has now become accepted. Subtype and Stage Inference (SuStaIn) distinguished four distinct spatiotemporal trajectories of pathology: limbic (S1), medial temporal lobe‐sparing (S2), posterior (S3), lateral (S4). A visual method to validate identify them is a requirement for their clinical translation. Our study aims provide evidence multiple setting by developing testing novel...
Abstract Background Substantial variability in tau accumulation patterns Alzheimer’s disease (AD) population has now become accepted. Subtype and Stage Inference (SuStaIn) distinguished four distinct spatiotemporal trajectories of pathology: limbic (S1), medial temporal lobe‐sparing (S2), posterior (S3), lateral (S4). A visual method to validate identify them is a requirement for their clinical translation. Our study aims provide evidence multiple setting by developing testing novel...
Abstract Background Cerebral small vessel disease (SVD) is a cerebrovascular condition associated with age that contributes to dementia, stroke, and other neurological disorders. White matter lesions (WML), microbleeds, infarcts are hallmarks of SVD, but their molecular mechanisms poorly understood. The aim this study was investigate the CSF proteomic changes underlying pathogenesis SVD subtypes. Method We analysed samples controls patients (295 WML, 149 microbleeds 64 infarcts, total 894...