A5024323677- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Liver Disease Diagnosis and Treatment
- PI3K/AKT/mTOR signaling in cancer
- Hepatitis C virus research
- Hepatitis B Virus Studies
- Advanced Biosensing Techniques and Applications
- Complement system in diseases
- Glycosylation and Glycoproteins Research
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Cancer Mechanisms and Therapy
- Microfluidic and Bio-sensing Technologies
- Liver physiology and pathology
- Chromosomal and Genetic Variations
- Immune Response and Inflammation
- Diabetes and associated disorders
- Cytomegalovirus and herpesvirus research
- Cell Adhesion Molecules Research
- Nanofabrication and Lithography Techniques
- Drug-Induced Hepatotoxicity and Protection
- Vitamin D Research Studies
- Hepatocellular Carcinoma Treatment and Prognosis
Inserm
2016-2025
Université Grenoble Alpes
2016-2025
Centre National de la Recherche Scientifique
2003-2025
Institut pour l'avancée des biosciences
2013-2023
Institut de Biosciences et Biotechnologies
2013-2022
Agir Pour les Maladies Chroniques
2021
Advance (Japan)
2020
Centre Hospitalier Universitaire d'Angers
1999-2017
Université Joseph Fourier
2005-2016
Centre Hospitalier Universitaire de Grenoble
2003-2015
Abstract Hepatocellular carcinoma (HCC) can have viral or non-viral causes 1–5 . Non-alcoholic steatohepatitis (NASH) is an important driver of HCC. Immunotherapy has been approved for treating HCC, but biomarker-based stratification patients optimal response to therapy unmet need 6,7 Here we report the progressive accumulation exhausted, unconventionally activated CD8 + PD1 T cells in NASH-affected livers. In preclinical models NASH-induced therapeutic immunotherapy targeted at programmed...
Multiple sclerosis-associated retroviral element (MSRV) is a element, the sequence of which served to define W family human endogenous retroviruses. MSRV viral particles display proinflammatory activities both in vitro mononuclear cell cultures and vivo humanized SCID mice model. To understand molecular basis such properties, we have investigated inflammatory potential surface unit envelope protein (ENV-SU), fraction that poised naturally interact with host cells. We report this study ENV-SU...
Abstract Background Cobalt-ferrite nanoparticles (Co-Fe NPs) are attractive for nanotechnology-based therapies. Thus, exploring their effect on viability of seven different cell lines representing organs the human body is highly important. Methods The toxicological effects Co-Fe NPs were studied by in-vitro exposure A549 and NCIH441 cell-lines (lung), precision-cut lung slices from rat, HepG2 cell-line (liver), MDCK (kidney), Caco-2 TC7 (intestine), TK6 (lymphoblasts) primary mouse...
Abstract New, ultrasmall nanoparticles with sizes below 5 nm have been obtained. These small rigid platforms (SRP) are composed of a polysiloxane matrix DOTAGA (1,4,7,10‐tetraazacyclododecane‐1‐glutaric anhydride‐4,7,10‐triacetic acid)–Gd 3+ chelates on their surface. They synthesised by an original top‐down process: 1) formation gadolinium oxide Gd 2 O 3 core, 2) encapsulation in shell grafted ligands, 3) dissolution the core due to chelation ligands and 4) fragmentation. fully...
Multiple sclerosis (MS) is a complex multifactorial disease of the central nervous system (CNS) for which animal models have mainly addressed downstream immunopathology but not potential inducers autoimmunity. In absence pathogen known to cause neuroinflammation in MS, Mycobacterial lysate commonly used form complete Freund's adjuvant induce autoimmunity myelin proteins Experimental Allergic Encephalomyelitis (EAE), an model MS. The present study demonstrates that protein from human...
Understanding how hepatitis C virus (HCV) induces and circumvents the host's natural killer (NK) cell-mediated immunity is of critical importance in efforts to design effective therapeutics. We report here decreased expression NKG2D activating receptor as a novel strategy adopted by HCV evade NK-cell mediated responses. show that chronic infection associated with ligands for NKG2D, MHC class I-related Chain (MIC) molecules, on hepatocytes. However, downmodulated circulating NK cells,...
The toxicological effects of cobalt nanoparticles (Co-NPs) aggregates were examined and compared with those ions (Co-ions) using six different cell lines representing lung, liver, kidney, intestine, the immune system. Dose-response curves studied in concentration range 0.05–1.0mM, employing 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide test, neutral red, Alamar blue as end point assays following exposures for 48 72 h. Data analysis predictive modeling obtained data sets...
The MSRV (multiple sclerosis-associated retrovirus) belongs to the human endogenous retrovirus HERV-W family. envelope protein originating from has been found in most patients with multiple sclerosis (MS). This (Env-ms) pro-inflammatory properties for several types of immune cells and could therefore play a role MS pathogenesis by promoting leukocyte diapedesis observed central nervous system patients. Our study aims analyze effects Env-ms on blood-brain barrier (BBB) at molecular functional...
Hepatocellular carcinoma (HCC) is the most common type of liver cancer. The majority HCC cases are associated with fibrosis or cirrhosis developing from chronic injuries. immune system contributes to severity tissue damage, establishment and disease's progression towards HCC. Herein, we provide a detailed characterization DEN-induced rat model during development special focus on liver's inflammatory microenvironment. Fischer 344 male rats were treated weekly for 14 weeks intra-peritoneal...
Rab6 is a small GTP-binding protein that belongs to the Ras superfamily and involved in intra-Golgi transport. Using two-hybrid system screen of mouse brain cDNA library, we have isolated several clones encoding proteins interact with Rab6. Approximately 60% identified encoded new Rab GDP dissociation inhibitor (GDI) isoform. This GDI isoform distinct from mGDI-1 mGDI-2, which been characterized previously, most likely represents counterpart rat GDIβ In system, interacts wild-type Rab5, but...
Sorting and recovering specific live cells from samples containing less than a few thousand have become major hurdles in rare cell exploration such as stem research, therapy based diagnostics. We describe here new technology on microelectronic chip integrating an array of over 100,000 independent electrodes sensors which allow individual parallel single manipulation up to 10,000 while maintaining viability proliferation capabilities. Manipulation is carried out using dynamic...
Dendritic cells are known to be activated by a wide range of microbial products, leading cytokine production and increased levels membrane markers such as major histocompatibility complex class II molecules. Such dendritic possess the capacity activate naïve T cells. In present study we demonstrated that immature secrete both YM1 lectin lipocalin-2. By testing ligands these two proteins, chitosan siderophores, respectively, also chitosan, degradation product various fungal protozoal cell...
Several optical surface sensing techniques, such as Surface Plasmon Resonance (SPR), work by imaging the base of a prism one its faces. However, fundamental concern has not been fully analyzed and understood so far, spatial resolution remains critical controversial issue. In SPR, propagation length L(x) plasmon waves considered limiting factor. Here, we demonstrate that for unoptimized systems geometrical aberrations caused can be more than length. By combining line-scan mode with optimized...
HIV is one of the deadliest pandemics modern times, having already caused 35 million deaths around world. Despite huge efforts spent to develop treatments, virus cannot yet be eradicated and continues infect new people. Spread remains uncontrolled, thus exposing worldwide population danger, due lack efficient vaccines. The latest clinical trials describe challenges associated with developing an effective prophylactic vaccine. These immunological obstacles will only overcome by smart...