A5022293812- Gastric Cancer Management and Outcomes
- Colorectal Cancer Treatments and Studies
- Liver Disease Diagnosis and Treatment
- Esophageal Cancer Research and Treatment
- Photovoltaic System Optimization Techniques
- Silicon and Solar Cell Technologies
- Genetic factors in colorectal cancer
- Liver physiology and pathology
- Electronic Packaging and Soldering Technologies
- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Cancer, Lipids, and Metabolism
- Colorectal Cancer Screening and Detection
- Cancer Immunotherapy and Biomarkers
- Advanced MEMS and NEMS Technologies
- Metastasis and carcinoma case studies
- Cancer, Hypoxia, and Metabolism
- Caveolin-1 and cellular processes
- Liver Disease and Transplantation
- Helicobacter pylori-related gastroenterology studies
- Advanced Proteomics Techniques and Applications
- Advanced Surface Polishing Techniques
- Epigenetics and DNA Methylation
- Hepatitis B Virus Studies
- Gastrointestinal Tumor Research and Treatment
University Medical Centre Mannheim
2016-2025
Heidelberg University
2016-2025
University Hospital Heidelberg
2016-2025
University Medical Center
2020-2025
Heidelberg University
2025
European Molecular Biology Laboratory
2024-2025
University of Mannheim
2015-2025
Medizinische Fakultät Mannheim
2012-2024
Cancer Institute (WIA)
2024
Fraunhofer Center for Silicon Photovoltaics
2010-2023
Abstract Hepatocellular carcinoma (HCC) can have viral or non-viral causes 1–5 . Non-alcoholic steatohepatitis (NASH) is an important driver of HCC. Immunotherapy has been approved for treating HCC, but biomarker-based stratification patients optimal response to therapy unmet need 6,7 Here we report the progressive accumulation exhausted, unconventionally activated CD8 + PD1 T cells in NASH-affected livers. In preclinical models NASH-induced therapeutic immunotherapy targeted at programmed...
Clinical laboratory testing for HER2 status in breast cancer tissues is critically important therapeutic decision making. Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) a powerful tool investigating proteins through the direct and morphology-driven analysis of tissue sections. We hypothesized that MALDI-IMS may determine directly from tissues. Breast (n = 48) predefined were subjected to MALDI-IMS, protein profiles obtained Protein identification was...
Proteomics analyses have been exploited for the discovery of novel biomarkers early recognition and prognostic stratification cancer patients. These now extended to whole tissue sections by using a new tool, that is, MALDI imaging. This allows spatial resolution protein peptides their allocation histoanatomical structures. Each imaging data set contains large number proteins peptides, analysis can be quite tedious. We report here approach results. Mass spectra are classified hierarchical...
The ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) is a regulating cellular levels, recently suggested as tumor suppressor. However, the role of UCHL1 in hepatocellular carcinoma (HCC) not clear. We investigated expression and DNA methylation primary HCC, liver metastases from digestive carcinomas, cancers. expressed all normal tissues immortalized epithelial cell lines, but was low or silenced 77% (10/13) HCC which well correlated with its promoter status. Methylation further detected 44%...
In clinical diagnostics, it is of outmost importance to correctly identify the source a metastatic tumor, especially if no apparent primary tumor present. Tissue-based proteomics might allow correct classification. As result, we performed MALDI imaging generate proteomic signatures for different tumors. These were used classify common cancer types. At first, cohort comprised tissue samples from six adenocarcinoma entities located at organ sites (esophagus, breast, colon, liver, stomach,...
Although metabolism is profoundly altered in human liver cancer, the extent to which experimental models, e.g. cell lines, mimic those alterations unresolved. Here, we aimed determine resemblance of hepatocellular carcinoma (HCC) lines tumours, specifically expression deregulated metabolic targets clinical tissue samples.We compared overall gene profile poorly-differentiated (HLE, HLF, SNU-449) well-differentiated (HUH7, HEPG2, HEP3B) HCC three publicly available microarray datasets. Three...
In this study we used differentiated adult human upcyte® cells for the in vitro generation of liver organoids. Upcyte® are genetically engineered cell strains derived from primary by lenti-viral transduction genes or gene combinations inducing transient proliferation capacity (upcyte® process). Proliferating undergo a finite number divisions, i.e., 20 to 40 population doublings, but upon withdrawal stimulating factors, they regain most specific characteristics cells. When defined mixture...
Background & AimsPancreatic ductal adenocarcinoma (PDAC) is frequently accompanied by excruciating pain, which has been associated with attraction of cancer cells and their invasion intrapancreatic sensory nerves. Neutralization the chemokine CCL2 reduced cancer-associated pain in a clinical trial, but there have no systematic analyses highly diverse families receptors PDAC.MethodsWe performed an open, unbiased RNA-interference screen mammalian chemokines co-cultures mouse PDAC (K8484)...
The aim of this study was to identify clinical risk factors for antimicrobial resistances and multidrug resistance (MDR) in urinary tract infections (UTI) an emergency department order improve empirical therapy. UTI cases from (ED) during January 2013 June 2015 were analyzed. Differences between patients with without towards Ciprofloxacin, Piperacillin Tazobactam (Pip/taz), Gentamicin, Cefuroxime, Cefpodoxime Ceftazidime analyzed Fisher's exact tests. Results used logistic regression...
BackgroundResponse to immunotherapy in gastric cancer is associated with microsatellite instability (or mismatch repair deficiency) and Epstein-Barr virus (EBV) positivity. We therefore aimed develop validate deep learning-based classifiers detect EBV status from routine histology slides.MethodsIn this retrospective, multicentre study, we collected tissue samples ten cohorts of patients seven countries (South Korea, Switzerland, Japan, Italy, Germany, the UK USA). trained a classifier...
Die Experten haben die gesamte Leitlinie durchgesehen und auf Aktualisierungsbedarf geprüft. Folgende Änderungen sich im Vergleich zur vorherigen von 2011 ergeben:
4502 Background: Perioperative FLOT is a standard of care for resectable, esophagogastric adenocarcinoma (EGA). This trial evaluates the addition trastuzumab (tras) and pertuzumab (per) to HER2-positive resectable patients (pts). Methods: PETRARCA prospective, multicenter, randomized, investigator initiated planned as phase II/III investigation. We report II part this trial. Pts with HER2+ EGA (≥ cT2 or cN+) were enrolled. randomized 1:1 4 pre- post-operative cycles (Docetaxel 50 mg/m²;...
β-Catenin is critical for intercellular adhesion and also plays a role as transcription activating protein in the Wnt signalling pathway. Increased levels mutation of β-catenin gene have been demonstrated various cancers; however, gastric cancer remains largely unknown. Using tissues normal adjacent mucosa obtained from 20 patients with (eight diffuse-type, 12 intestinal-type) undergoing resection or endoscopy, we assessed expression by immunohistochemistry quantitative PCR analysis....
Background— Increased levels of inflammatory markers are predictors thromboembolic events during atrial fibrillation (AF). endocardial expression adhesion molecules (ie, vascular cell molecule [VCAM] and intercellular [ICAM]) could be an important link between initiation prothrombogenic mechanisms responsible for thrombus development at the endocardium (endocardial remodeling). Methods Results— Tissue microarrays were used to screen right tissue specimens obtained from 320 consecutive...