A5009831262- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Single-cell and spatial transcriptomics
- Immunotherapy and Immune Responses
- COVID-19 Clinical Research Studies
- Antimicrobial Peptides and Activities
- Complex Network Analysis Techniques
- Public Relations and Crisis Communication
- Immune cells in cancer
- Semantic Web and Ontologies
- CAR-T cell therapy research
- Osteoarthritis Treatment and Mechanisms
- Cancer Immunotherapy and Biomarkers
- Cell Image Analysis Techniques
- Pancreatic function and diabetes
- IL-33, ST2, and ILC Pathways
- Chemical Synthesis and Analysis
- Bone health and treatments
- Human Mobility and Location-Based Analysis
- Asthma and respiratory diseases
- Biochemical and Structural Characterization
- Immune responses and vaccinations
- Atherosclerosis and Cardiovascular Diseases
- Advanced Text Analysis Techniques
German Rheumatism Research Centre
2015-2024
Leibniz Association
2010-2024
Philipps University of Marburg
2021-2024
Ludwig-Maximilians-Universität München
2024
University of Göttingen
1988-2022
Technical University of Darmstadt
2012-2021
Zero to Three
2020
Orthopädische Praxis
2007-2019
Kunststoff-Institut Lüdenscheid
2018
Charité - Universitätsmedizin Berlin
2011-2017
We report the isolation and characterization of a novel human peptide with antimicrobial activity, termed LEAP‐1 (liver‐expressed peptide). Using mass spectrometric assay detecting cysteine‐rich peptides, 25‐residue containing four disulfide bonds was identified in blood ultrafiltrate. expression predominantly detected liver, and, to much lower extent, heart. In radial diffusion assays, Gram‐positive Bacillus megaterium , subtilis Micrococcus luteus Staphylococcus carnosus Gram‐negative...
Abstract Hepatocellular carcinoma (HCC) can have viral or non-viral causes 1–5 . Non-alcoholic steatohepatitis (NASH) is an important driver of HCC. Immunotherapy has been approved for treating HCC, but biomarker-based stratification patients optimal response to therapy unmet need 6,7 Here we report the progressive accumulation exhausted, unconventionally activated CD8 + PD1 T cells in NASH-affected livers. In preclinical models NASH-induced therapeutic immunotherapy targeted at programmed...
Severe COVID-19 is linked to both dysfunctional immune response and unrestrained immunopathology, it remains unclear whether T cells contribute disease pathology. Here, we combined single-cell transcriptomics proteomics with mechanistic studies assess pathogenic cell functions inducing signals. We identified highly activated CD16
Real-time information from microblogs like Twitter is useful for different applications such as market research, opinion mining, and crisis management. For many of those messages, location required to derive insights. Today, however, only around 1% all tweets are explicitly geotagged. We propose the first multi-indicator method determining (1) where a tweet was created well (2) user's residence. Our based on various weighted indicators, including names places that appear in text message,...
Abstract Mass cytometry is developing as a means of multiparametric single-cell analysis. In this study, we present an approach to barcoding separate live human PBMC samples for combined preparation and acquisition on by time flight instrument. Using six different anti-CD45 Ab conjugates labeled with Pd104, Pd106, Pd108, Pd110, In113, In115, respectively, barcoded up 20 unique combinations exactly three CD45 tags. Cell events carrying more than or less tags were excluded from analyses during...
Abstract Immunological competence declines progressively with age, resulting in increased susceptibility of the elderly to infection and impaired responses vaccines. Underlying mechanisms remain largely obscure as they have been related complex, individual systemic immune properties that are challenging investigate. In this study, we explored age-related changes human immunity during a primary virus experimentally induced by immunization live-attenuated yellow fever (YF) vaccine. Applying...
Abstract Immune-mediated inflammatory diseases (IMIDs) are typically characterised by relapsing and remitting flares of inflammation. However, the unpredictability disease impedes their study. Addressing this critical knowledge gap, we use experimental medicine approach immunomodulatory drug withdrawal in rheumatoid arthritis (RA) remission to synchronise flare processes allowing detailed characterisation. Exploratory mass cytometry analyses reveal three circulating cellular subsets...
Significance CLL is characterized by autonomous B cell receptor (BCR) signaling. subsets are empirically defined sequence similarities of the BCR heavy chain. However, in unfavorable subset 2, an acquired mutation (termed R110) light chain stimulates This study demonstrates that oncogenic R110 dictates prognosis and not restricted to conventional 2. Interestingly, carriers a particular light-chain allele ( IGLV3-21 * 01 ) predisposed develop because this enables signaling as single-point...
BackgroundSARS-CoV-2 specific antibody-secreting plasma cells (PC) mediating humoral immunity have been identified in the human bone marrow (BM) after COVID-19 or vaccination against SARS-CoV-2. However, it remained unclear whether not they acquire phenotypes of memory cells.MethodsSARS-CoV-2-specific (BMPC) were characterised by tetramer-based, antigen-specific flow cytometry and FluoroSpot assay.FindingsSARS-CoV-2 spike-S1-specific PC detectable all tested BM samples previously vaccinated...
Defensins are a family of secreted antimicrobial peptides proposed to directly interfere with bacterial membranes. Here we show functional analysis the novel beta-defensin DEFB123. A peptide comprising core region was synthesized and used for our analysis. Like other beta-defensins, DEFB123 exerted activity against broad spectrum Gram-positive Gram-negative bacteria, which assessed by microbroth dilution assay radial diffusion zone assay. In addition, showed lipopolysaccharide (LPS)-binding...
Mass cytometry is increasingly employed in larger immune profiling studies involving data acquisitions across several days and multiple sites. For gaining a maximum of information from respective by computational analyses, techniques have been developed to minimize noise mass cytometric sets, such as sample banking, standardized instrument setup, barcoding, signal normalization. However, the repeated preparation cocktails composed isotope-tagged antibodies remained significant source error....
Multiple sclerosis (MS) is an inflammatory demyelinating and neurodegenerative disease of the central nervous system (CNS). Studies in rodent models demonstrated association CNS-infiltrating monocyte-derived macrophages with severity. However, little known about humans. Here, we performed exploratory analysis peripheral blood mononuclear cells (PBMCs) isolated from healthy controls drug-naïve patients early MS using multiplexed single-cell mass cytometry algorithm-based data analysis. Two...