Martin Witzenrath
A5048444402- Pneumonia and Respiratory Infections
- COVID-19 Clinical Research Studies
- Respiratory Support and Mechanisms
- Long-Term Effects of COVID-19
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Neonatal Respiratory Health Research
- Immune Response and Inflammation
- Pneumocystis jirovecii pneumonia detection and treatment
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Asthma and respiratory diseases
- Inhalation and Respiratory Drug Delivery
- Intensive Care Unit Cognitive Disorders
- Sepsis Diagnosis and Treatment
- Pediatric health and respiratory diseases
- Pulmonary Hypertension Research and Treatments
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Bacteriophages and microbial interactions
- Nosocomial Infections in ICU
- Inflammasome and immune disorders
- Antibiotic Resistance in Bacteria
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Lipid metabolism and disorders
- Bacterial Infections and Vaccines
- Inflammation biomarkers and pathways
Charité - Universitätsmedizin Berlin
2016-2025
Humboldt-Universität zu Berlin
2009-2025
German Center for Lung Research
2017-2025
Freie Universität Berlin
2017-2025
Friedrich Schiller University Jena
2024
Jena University Hospital
2024
German Respiratory Society
2023
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2020-2023
Justus-Liebig-Universität Gießen
2001-2021
Orthopädische Universitätsklinik
2014-2021
To investigate the immune response and mechanisms associated with severe coronavirus disease 2019 (COVID-19), we performed single-cell RNA sequencing on nasopharyngeal bronchial samples from 19 clinically well-characterized patients moderate or critical five healthy controls. We identified airway epithelial cell types states vulnerable to acute respiratory syndrome 2 (SARS-CoV-2) infection. In COVID-19, cells showed an average three-fold increase in expression of SARS-CoV-2 entry receptor...
The COVID-19 pandemic is an unprecedented global challenge, and point-of-care diagnostic classifiers are urgently required. Here, we present a platform for ultra-high-throughput serum plasma proteomics that builds on ISO13485 standardization to facilitate simple implementation in regulated clinical laboratories. Our low-cost workflow handles up 180 samples per day, enables high precision quantification, reduces batch effects large-scale longitudinal studies. We use our collected from cohort...
The emergence of SARS-CoV-2 led to pandemic spread coronavirus disease 2019 (COVID-19), manifesting with respiratory symptoms and multi-organ dysfunction. Detailed characterization virus-neutralizing antibodies target epitopes is needed understand COVID-19 pathophysiology guide immunization strategies. Among 598 human monoclonal (mAbs) from 10 patients, we identified 40 strongly neutralizing mAbs. most potent mAb, CV07-209, neutralized authentic an IC50 value 3.1 ng/mL. Crystal structures...
Abstract Streptococcus pneumoniae is a leading cause of pneumonia, meningitis, and sepsis. Pneumococci can be divided into >90 serotypes that show differences in the pathogenicity invasiveness. We tested hypotheses innate immune inflammasome pathway involved fighting pneumococcal pneumonia some invasive types are not recognized by this pathway. human murine mononuclear cells responded to S. expressing hemolytic pneumolysin producing IL-1β. This IL-1β production depended on NOD-like...
Macrophages can be niches for bacterial pathogens or antibacterial effector cells depending on the pathogen and signals from immune system. Here we show that type I II IFNs are master regulators of gene expression during Legionella pneumophila infection, activators an alveolar macrophage-intrinsic response restricts growth pneumonia. Quantitative mass spectrometry revealed both substantially modify Legionella-containing vacuoles, comparative analyses reveal distinct subsets transcriptionally...
Summary Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a rapidly unfolding pandemic, overwhelming health care systems worldwide 1 . Clinical manifestations of Coronavirus-disease 2019 (COVID-19) vary broadly, ranging from asymptomatic infection to failure and death , yet the underlying mechanisms for this high variability are still unknown. Similarly, role host immune responses in viral clearance COVID-19 remains unresolved. For SARS-CoV (2002/03), however, it been...
In coronavirus disease 2019 (COVID-19), hypertension and cardiovascular diseases are major risk factors for critical progression. However, the underlying causes effects of main anti-hypertensive therapies—angiotensin-converting enzyme inhibitors (ACEIs) angiotensin receptor blockers (ARBs)—remain unclear. Combining clinical data (n = 144) single-cell sequencing airway samples 48) with in vitro experiments, we observed a distinct inflammatory predisposition immune cells patients that...
Advancements in methods, technology, and our understanding of the pathobiology lung injury have created need to update definition experimental acute (ALI). We queried 50 participants with expertise ALI respiratory distress syndrome using a Delphi method composed series electronic surveys virtual workshop. propose that presents as “multidimensional entity” characterized by four “domains” reflect key pathophysiologic features underlying biology human syndrome. These domains are 1) histological...
Patients diagnosed with coronavirus disease 2019 (COVID-19) associated severe acute respiratory syndrome 2 (SARS-CoV-2) infection frequently experience symptom burden post-acute or post-hospitalisation. We aimed to identify optimal strategies for follow-up care that may positively impact the patient's quality of life (QoL). A European Respiratory Society (ERS) Task Force convened and prioritised eight clinical questions. targeted search literature defined timeline “long COVID” as 1–6 months...
While much of the attention on COVID-19 pandemic was directed at daily counts cases and those with serious disease overwhelming health services, increasingly, reports have appeared people who experience debilitating symptoms after initial infection. This is popularly known as long COVID.
Abstract Vaccines play a critical role in combating the COVID-19 pandemic. Future control of pandemic requires improved vaccines with high efficacy against newly emerging SARS-CoV-2 variants and ability to reduce virus transmission. Here we compare immune responses preclinical mRNA vaccine BNT162b2, adenovirus-vectored spike Ad2-spike live-attenuated candidate sCPD9 Syrian hamsters, using both homogeneous heterologous vaccination regimens. Comparative was assessed by employing readouts from...
Rationale: Systemic sclerosis (SSc)-associated pulmonary arterial hypertension (PAH) portends worse outcome than other forms of PAH. Vasoconstrictive and vascular remodeling actions endothelin (ET) 1 angiotensin (Ang) II via receptor type A (ETAR) Ang type-1 (AT1R) activation are implicated in PAH pathogenesis.Objectives: We hypothesized that stimulating autoantibodies (Abs) targeting activating AT1R ETAR may contribute to SSc-PAH pathogenesis, tested their functional biomarker...
Abstract Different NOD-like receptors, including NLRP1, NLRP3, and NLRC4, as well the recently identified HIN-200 protein, AIM2, form multiprotein complexes called inflammasomes, which mediate caspase-1–dependent processing of pro-IL-1β. Listeria monocytogenes is an intracellular pathogen that actively phagocytosed by monocytes/macrophages subsequently escapes from phagosome into host cell cytosol, depending on its pore-forming toxin listeriolysin O (LLO). In this study, we demonstrate human...
Vasculopathy, inflammatory fibrosis and functional autoantibodies (Abs) are major manifestations of systemic sclerosis (SSc). Abs directed against the angiotensin II type 1 receptor (AT₁R) endothelin-1 A (ETAR) associated with characteristic disease features including vascular, inflammatory, fibrotic complications indicating their role in SSc pathogenesis. Therefore, impact anti-AT₁R anti-ETAR on initiation inflammation was analyzed.Anti-AT₁R Ab-positive immunoglobulin G (IgG) from patients...
Hypoxic pulmonary vasoconstriction (HPV) is a physiological mechanism by which arteries constrict in hypoxic lung areas order to redirect blood flow with greater oxygen supply. Both sensing and the contractile response are thought be intrinsic arterial smooth muscle cells. Here we speculated that ideal site for might instead at alveolocapillary level, subsequent retrograde propagation upstream arterioles via connexin 40 (Cx40) endothelial gap junctions. HPV was largely attenuated...