A5038374049- Bacterial Infections and Vaccines
- COVID-19 Clinical Research Studies
- Infectious Encephalopathies and Encephalitis
- SARS-CoV-2 and COVID-19 Research
- Pneumonia and Respiratory Infections
- Long-Term Effects of COVID-19
- COVID-19 Impact on Reproduction
- Bacteriophages and microbial interactions
- Infectious Diseases and Tuberculosis
- Polyomavirus and related diseases
- S100 Proteins and Annexins
- Enterobacteriaceae and Cronobacter Research
- Streptococcal Infections and Treatments
- Mycobacterium research and diagnosis
- Complement system in diseases
- Mechanical Circulatory Support Devices
- Cerebrospinal fluid and hydrocephalus
- Respiratory viral infections research
- Stress Responses and Cortisol
- Respiratory Support and Mechanisms
- Infective Endocarditis Diagnosis and Management
- RNA Research and Splicing
- Neonatal and Maternal Infections
- Animal Virus Infections Studies
- Plant Virus Research Studies
Amsterdam Neuroscience
2019-2024
Amsterdam University Medical Centers
2019-2024
University of Amsterdam
2019-2024
We aimed to determine diagnostic accuracy of inflammatory markers in plasma and cerebrospinal fluid (CSF) for the diagnosis central nervous system (CNS) infections specifically bacterial meningitis.
Identifying the causative pathogen in central nervous system (CNS) infections is crucial for patient management and prognosis. Many viruses can cause CNS infections, yet screening each individually costly time-consuming. Most metagenomic assays theoretically detect all pathogens, but often fail to because of their small genome low viral load. Viral metagenomics overcomes this by enrichment genomic content a sample. VIDISCA-NGS one available workflows metagenomics, which requires only input...
BackgroundThe diagnosis of meningitis in nonsurgical hospitalised patients is often difficult and diagnostic accuracy clinical, laboratory, radiological characteristics unknown.MethodsIn a prospective multi-centre cohort study the Netherlands with adults suspected central nervous system (CNS) infections, we included consecutive who underwent lumbar puncture for suspicion nosocomial CNS infection. All episodes were categorized into five final clinical categories, as reference standard:...
In patients with coronavirus disease 2019 (COVID-19), men are more severely affected than women. Multiple studies suggest that androgens might play a role in this difference severity. Our objective was to assess the association between sex hormone levels and mortality severe COVID-19.We selected from Amsterdam University Medical Centers COVID-19 Biobank, which admitted hospital March April 2020, reverse transcription-polymerase chain reaction proven acute respiratory syndrome-coronavirus-2...
Abstract Purpose Cerebrospinal fluid (CSF) granulocytes are associated with bacterial meningitis, but information on its diagnostic value is limited and primarily based retrospective studies. Therefore, we assessed the accuracy of CSF granulocytes. Methods We analyzed (index test) from all consecutive patients in two prospective cohort studies Netherlands. Both included ≥ 16 years, suspected a central nervous system (CNS) infection, who underwent lumbar puncture. All episodes elevated...
Abstract Diagnosing central nervous system (CNS) infections quickly is often difficult. Neurofilament light chain (NfL) a component of the axonal cytoskeleton and identified as marker neuronal damage in several CNS diseases. We evaluated diagnostic accuracy NfL for diagnosing infections. included patients from prospective cohort consecutive whom lumbar puncture was performed suspected infection an academic hospital The Netherlands. index test cerebrospinal fluid (CSF) reference standard...
Vilobelimab, a complement 5a (C5a)-specific monoclonal antibody, reduced mortality in critically ill COVID-19 patients phase 3 multicentre, randomized, double-blind, placebo-controlled study. As part of the study, vilobelimab concentrations and C5a levels as well antidrug antibodies (ADAs) to were analysed.From Oct 1, 2020 4, 2021, 368 invasively mechanically ventilated randomized: 177 randomly assigned receive while 191 received placebo. Pharmacokinetic sampling was only performed at sites...
ObjectivesConfirming the diagnosis in viral central nervous system (CNS) infections can be difficult with currently available diagnostic tools. Virus discovery cDNA-amplified fragment length polymorphism next-generation sequencing (VIDISCA-NGS) is a promising metagenomic technique that enables detection of all viruses single assay. We performed retrospective study on accuracy VIDISCA-NGS cerebrospinal fluid (CSF) individuals suspected CNS infections.MethodsConsecutive adult patients...
Abstract In a phase 3 trial (PANAMO, NCT04333420), vilobelimab, complement 5a (C5a) inhibitor, reduced 28-day mortality in mechanically ventilated COVID-19 patients. This post hoc analysis of 368 patients aimed to explore treatment heterogeneity through unsupervised learning. All available clinical variables at baseline were used as input. Treatment was assessed using latent class (LCA), Ward’s hierarchical clustering (HC) and the adjudication previously described sepsis phenotypes. The...
Abstract Background Seizures can be part of the clinical presentation central nervous system (CNS) infections. We describe patients suspected a neurological infection who present with seizure and study diagnostic accuracy laboratory features predictive CNS in this population. Methods analyzed all consecutive presenting from two prospective Dutch cohort studies, which were included underwent cerebrospinal fluid (CSF) examination because suspicion infection. Results Of 900 episodes infection,...
Background: Severe coronavirus disease 2019 (Covid-19) is characterized by inflammation and coagulation in the presence of complement activation. Methods: We conducted an explorative phase 2 randomized, open label first part adaptive 2/3 trial intravenous IFX-1, a monoclonal antibody selectively blocking anaphylatoxin C5a, adults with severe Covid-19. Patients were randomized between IFX-1 plus best supportive care (BSC) or BSC only. Results: 30 patients underwent randomization: 15 assigned...
ABSTRACT POPDC2 encodes for the Popeye domain-containing protein 2 which has an important role in cardiac pacemaking and conduction, due part to its cAMP-dependent binding regulation of TREK-1 potassium channels. Loss Popdc2 mice results sinus pauses bradycardia morpholino knockdown popdc2 zebrafish atrioventricular (AV) block. We identified bi-allelic variants 4 families that presented with a phenotypic spectrum consisting node dysfunction, AV conduction defects hypertrophic cardiomyopathy....
Abstract Suspected central nervous system (CNS) infections may pose a diagnostic challenge, and often concern severely ill patients. We aim to identify predictors of unfavourable outcome prioritize diagnostics treatment improvements. Unfavourable was assessed on the Glasgow Outcome Scale at hospital discharge, defined by score 1 4. Of 1152 episodes with suspected CNS infection, from two Dutch prospective cohorts, median age 54 (IQR 37–67), 563 (49%) occurred in women. The final diagnoses...
Abstract Background In patients with central nervous system (CNS) infections identification of the causative pathogen is important for treatment. Metagenomic next-generation sequencing techniques are increasingly being applied to identify causes CNS infections, as they can detect any nucleic acid sequences present. Viromic that enrich samples virus particles prior may simultaneously ribosomes from bacterial pathogens, which similar in size small viruses. Methods We studied performance a...