A5089849107- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- HIV Research and Treatment
- Virus-based gene therapy research
- Respiratory viral infections research
- Viral Infections and Immunology Research
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Bacteriophages and microbial interactions
- Animal Disease Management and Epidemiology
- Plant Virus Research Studies
- Influenza Virus Research Studies
- Viral Infections and Vectors
- COVID-19 epidemiological studies
- Virology and Viral Diseases
- Immune Cell Function and Interaction
- SARS-CoV-2 detection and testing
- Cytomegalovirus and herpesvirus research
- Vector-Borne Animal Diseases
- Parvovirus B19 Infection Studies
- Mosquito-borne diseases and control
- Zoonotic diseases and public health
- Viral Infections and Outbreaks Research
University of Amsterdam
2015-2024
Amsterdam University Medical Centers
2018-2024
Amsterdam Institute for Addiction Research
2022-2023
University of Miami
2022
Amsterdam UMC Location University of Amsterdam
2011-2020
Netherlands Institute for Health Services Research
2014
Inmunal (Spain)
2010
Amsterdam University of the Arts
2007
MRC Laboratory of Molecular Biology
2007
University of Oxford
2007
Three human coronaviruses are known to exist: coronavirus 229E (HCoV-229E), HCoV-OC43 and severe acute respiratory syndrome (SARS)-associated (SARS-CoV). Here we report the identification of a fourth coronavirus, HCoV-NL63, using new method virus discovery. The was isolated from 7-month-old child suffering bronchiolitis conjunctivitis. complete genome sequence indicates that this is not recombinant, but rather group 1 coronavirus. in vitro host cell range HCoV-NL63 notable because it...
Coronavirus (CoV) infection of humans is usually not associated with severe disease. However, discovery the acute respiratory syndrome (SARS) CoV revealed that highly pathogenic human CoVs (HCoVs) can evolve. The identification and characterization new HCoVs is, therefore, an important task. Recently, a HCoV termed NL63 was discovered in patients tract illness. Here, cell tropism receptor usage HCoV-NL63 were analyzed. spike (S) protein mediated different target cells compared closely...
A key unsolved question in the current coronavirus disease 2019 (COVID-19) pandemic is duration of acquired immunity. Insights from infections with four seasonal human coronaviruses might reveal common characteristics applicable to all coronaviruses. We monitored healthy individuals for more than 35 years and determined that reinfection same occurred frequently at 12 months after infection.
Since 2009 pandemic, international health authorities recommended monitoring severe and complicated cases of respiratory disease, that is, acute infection (SARI) distress syndrome (ARDS). We evaluated the proportion SARI/ARDS deaths due to influenza A(H1N1)pdm09 impact other viruses during pandemic postpandemic period (2009–2011) in northern Italy; additionally we searched for unknown those which diagnosis remained negative. 206 samples were collected from analyzed by real-time RT-PCR/PCR...
The type II transmembrane protease TMPRSS2 activates the spike (S) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) on cell surface following receptor binding during viral entry into cells. In absence TMPRSS2, SARS-CoV achieves via an endosomal pathway in which cathepsin L may play important role, i.e., activation fusogenicity. This study shows that a commercial serine inhibitor (camostat) partially blocked infection by and human NL63 (HCoV-NL63) HeLa cells expressing...
SARS-CoV, human coronavirus NL63 (HCoV-NL63) and HCoV-HKU1 were first described in 2003, 2004 2005 respectively. Nevertheless, discovery of three new coronaviruses does not necessary represent a sudden increase emerging infections by coronaviruses. Only SARS-CoV has recently been introduced to the population; other two have circulating humans for long time. HCoV-NL63 are respiratory coronaviruses, frequently found during lower upper tract infections, spread worldwide, prefer winter season....
ABSTRACT We previously showed that close relatives of human coronavirus 229E (HCoV-229E) exist in African bats. The small sample and limited genomic characterizations have prevented further analyses so far. Here, we tested 2,087 fecal specimens from 11 bat species sampled Ghana for HCoV-229E-related viruses by reverse transcription-PCR (RT-PCR). Only hipposiderid bats positive. To compare the genetic diversity HCoV-229E, historical isolates diagnostic globally over 10 years. Bat were 5-...
The family Coronaviridae includes viruses with positive-sense RNA genomes of 22–36 kb that are expressed through a nested set 3′ co-terminal subgenomic mRNAs. Members the subfamily Orthocoronavirinae characterized by 80–160 nm diameter, enveloped virions spike projections. orthocoronaviruses, severe acute respiratory syndrome coronavirus and Middle East syndrome-related extremely pathogenic for humans in last two decades have been responsible SARS MERS epidemics. Another orthocoronavirus, 2,...
The clinical relevance of infections with the novel human coronavirus NL63 (HCoV-NL63) has not been investigated systematically. We therefore determined its association disease in young children lower respiratory tract infection (LRTI).Nine hundred forty-nine samples nasopharyngeal secretions from under 3 y age LRTIs were analysed by a quantitative HCoV-NL63-specific real-time PCR. had collected hospitalised patients and outpatients December 1999 to October 2001 four different regions...
Abstract The human coronavirus NL63 (HCoV-NL63) was first identified in the Netherlands, and its circulation France has not been investigated. We studied HCoV-NL63 infection hospitalized children diagnosed with respiratory tract infections. From November 2002 to April 2003, we evaluated 300 specimens for HCoV-NL63. Of samples, 28 (9.3%) were positive highest prevalence found February (18%). main symptoms fever (61%), rhinitis (39%), bronchiolitis digestive problems (33%), otitis (28%),...
In 2004, the novel respiratory human coronavirus NL63 (HCoV-NL63) was identified, and subsequent research revealed that virus has spread worldwide. HCoV-229E is a close relative of HCoV-NL63, infection with either can lead to hospitalization young children, immunocompromised persons, elderly. Children infected HCoV-NL63 often develop croup, obstruction airway. this study we investigated at which age children are confronted for first time an and, thus, they seroconvert positivity. We designed...
In 2005, a human bocavirus was discovered in children with respiratory tract illnesses. Attempts to culture this virus on conventional cell lines has failed thus far. We investigated whether the can replicate pseudostratified airway epithelium. This system mimics environment and facilitates culturing of various agents. The cells were inoculated bocavirus-positive nasopharyngeal washes from children, replication monitored by measuring apical release via real-time PCR. Furthermore, we...
Four human coronaviruses are currently known to infect the respiratory tract: OC43 (HCoV-OC43) and 229E (HCoV-229E), SARS associated coronavirus (SARS-CoV) recently identified NL63 (HCoV-NL63). In this study we explored incidence of HCoV-NL63 infection in children diagnosed with tract infections Belgium. Samples from hospitalized diseases during winter seasons 2003 2004 were evaluated for presence using a optimized pancoronavirus RT-PCR assay. Seven positive samples identified, six collected...
The human airway epithelium (HAE) represents the entry port of many respiratory viruses, including coronaviruses (HCoVs). Nowadays, four HCoVs, HCoV-229E, HCoV-OC43, HCoV-HKU1, and HCoV-NL63, are known to be circulating worldwide, causing upper lower tract infections in nonhospitalized hospitalized children. Studies fundamental aspects these HCoV at primary port, such as cell tropism, seriously hampered by lack a universal culture system or suitable animal models. To expand knowledge on...
Like severe acute respiratory syndrome coronavirus (SARS-CoV), human (HCoV)-NL63 employs angiotensin-converting enzyme 2 (ACE2) as a receptor for cellular entry. SARS-CoV infection causes robust downregulation of ACE2 expression levels and it has been suggested that the effect on is involved in severity disease. We investigated whether occurs at optimal replication conditions HCoV-NL63 infection. The homologue ACE2, ACE protein not used by HCoV-NL63, was measured control. A specific decrease...
ABSTRACT Culturing newly identified human lung pathogens from clinical sample isolates can represent a daunting task, with problems ranging low levels of to the presence growth suppressive factors in specimens, compounded by lack suitable tissue culture system. However, it is critical develop vitro platforms isolate and characterize replication kinetics pathogenesis recently pathogens. HCoV-HKU1, coronavirus patient severe pneumonia, has been major challenge for successful propagation on all...
Human coronavirus NL63 (HCoV-NL63) is a globally endemic pathogen causing mild and severe respiratory tract infections with reinfections occurring repeatedly throughout lifetime.Nasal samples were collected in coastal Kenya through community-based hospital-based surveillance. HCoV-NL63 was detected multiplex real-time reverse transcription PCR, positive targeted for nucleotide sequencing of the spike (S) protein. Additionally, paired from 25 individuals evidence repeat infection selected...
Acute central nervous system (CNS) infections cause substantial morbidity and mortality, but the etiology remains unknown in a large proportion of cases. We identified characterized full genome novel cyclovirus (tentatively named cyclovirus-Vietnam [CyCV-VN]) cerebrospinal fluid (CSF) specimens two Vietnamese patients with CNS etiology. CyCV-VN was subsequently detected 4% 642 CSF from suspected none 122 CSFs noninfectious neurological disorders. Detection rates were similar those whom other...
Congenital tremor type A-II in piglets has been regarded as a transmissible disease since the 1970s, possibly caused by very recently-described virus: atypical porcine pestivirus (APPV). Here, we describe several strains of APPV with clinical signs congenital (10 10 farms tested). Piglets on farm no history were PCR-negative for virus. To demonstrate causal relationship between and disease, three gilts inoculated via intramuscular injection at day 32 pregnancy. In two litters, vertical...