A5060731465- Cervical Cancer and HPV Research
- Molecular Biology Techniques and Applications
- Reproductive tract infections research
- Genital Health and Disease
- Cell Adhesion Molecules Research
- Respiratory viral infections research
- Hepatitis B Virus Studies
- Angiogenesis and VEGF in Cancer
- Ocular Infections and Treatments
- Herpesvirus Infections and Treatments
- Global Cancer Incidence and Screening
- SARS-CoV-2 detection and testing
- HIV/AIDS drug development and treatment
- Viral gastroenteritis research and epidemiology
- Legionella and Acanthamoeba research
- HIV Research and Treatment
- Intraocular Surgery and Lenses
- Antifungal resistance and susceptibility
- Ocular Surface and Contact Lens
- Advanced biosensing and bioanalysis techniques
- Head and Neck Cancer Studies
- Platelet Disorders and Treatments
- Bacterial biofilms and quorum sensing
- Viral Infections and Immunology Research
- AI in cancer detection
University of Milano-Bicocca
2017-2025
University of Manitoba
2022
Public Health England
2018
University of Milan
2011-2017
Candiolo Cancer Institute
2010
Since 2009 pandemic, international health authorities recommended monitoring severe and complicated cases of respiratory disease, that is, acute infection (SARI) distress syndrome (ARDS). We evaluated the proportion SARI/ARDS deaths due to influenza A(H1N1)pdm09 impact other viruses during pandemic postpandemic period (2009–2011) in northern Italy; additionally we searched for unknown those which diagnosis remained negative. 206 samples were collected from analyzed by real-time RT-PCR/PCR...
ABSTRACT This study assessed the accuracy of high-risk human papillomavirus testing BD Onclarity HPV (Onclarity) assay on vaginal self-collected FLOQSwab versus cervical samples to ensure similar detect intraepithelial neoplasia. Testing was performed two automated platforms, Viper LT and COR, evaluate effect machine using self-samples analyze influence collection, transport, freezing-unfreezing results. A sample were collected from 300 women. The first tested LT, second swab frozen...
In the context of cervical cancer prevention, where human papillomavirus (HPV) infection is pivotal, HPV testing replacing Pap Smear in primary screening. This transition offers an opportunity for integrating self-sampling to enhance coverage. We evaluated accuracy using self-collected urine and vaginal samples, comparing them physician-collected swabs. From a cohort 245 women with abnormal cytology, we collected self-sampled vaginal, urine, clinician-administered specimens. Employing...
Background: Given the diagnostic accuracy of HPV-DNA tests in terms self-collected samples, order to implement self-sampling cervical screening programs, standardization pre-analytical phase, including decisions concerning choice medium, volume elution, and storage conditions, are necessary, addition understanding potential factors involved acceptability by women. On this basis, we carried out a cross-sectional study assess (i) stability dry vaginal samples stored at room temperature for up...
Wastewater-based epidemiology has emerged as a complementary tool for the monitoring of COVID-19 pandemic waves and circulation viral variants. The selection, standardization, dynamics different SARS-CoV-2 RNA targets in wastewater requires further investigation. In present study, 106 samples were collected over 24-month period from treatment plant Sondrio, north Italy, analyzed presence through quantification ORF1b, N1, N3 gene via one-step real-time qPCR. general, three demonstrated...
This study investigated the detection of high-risk Human Papillomavirus (hrHPV) and seven other pathogens associated with sexually transmitted infections (STIs) in matched clinician-collected cervical samples self-taken vaginal urine specimens collected from 342 asymptomatic women referred to colposcopy evaluate (i) concordance molecular pathogen three different sample types; (ii) analytical sensitivity specificity STIs on self-samples; (iii) distribution hrHPV-positive hrHPV-negative women....
Wastewater-based environmental surveillance enables the monitoring of SARS-CoV-2 dynamics within populations, offering critical epidemiological insights. Numerous workflows for tracking have been developed globally, underscoring need interlaboratory comparisons to ensure data consistency and comparability. An inter-calibration test was conducted among laboratories network in wastewater samples across Lombardy region (Italy). The aimed evaluate reliability identify potential sources...
The integrin alpha6 subunit is part of the alpha6beta1 and alpha6beta4 complexes, which are known to be receptors for laminins mediate several biological activities such as embryogenesis, organogenesis, invasion carcinoma cells. However, precise role in angiogenesis has not yet been addressed. We observed that both vascular endothelial growth factor-A fibroblast factor-2 strongly upregulate human Moreover, was positively modulated angiogenic vessels pancreatic neuroendocrine carcinoma. In...
Abstract Human papillomavirus (HPV) testing has been proposed as a means of replacing or supporting conventional cervical screening (Pap test). However, both methods require the collection samples. Urine sample is easier and more acceptable to collect could be helpful in facilitating cancer screening. The aim this study was evaluate sensitivity specificity urine compared smear using PCR‐based method with new, designed specifically primer set. Paired first voided samples collected from 107...
Circulating HPV DNA has been previously described in women with advanced stages of cervical cancer and suggested to be a prognostic marker disease recurrences metastases. Only few studies have reported the presence bloodstream patients low grade or precancerous lesions. This study aimed define if could detected plasma samples 120 referred for recent history dysplasia who presented lesions ranging from High Squamous Intraepithelial Lesion (H-SIL) regressed normal cytology. detection was...
The study purpose was to assess the efficacy of a preservative-free 0.6% povidone iodine eye drops as perioperative prophylactic treatment for reducing conjunctival bacterial load and rate needle contamination in patients undergoing intravitreal anti-vascular endothelial growth factor injection. Enrolled were randomized either group (0.6% iodine, three day-prophylactic before injection) or control (placebo, treatment). Conjunctival swabs obtained after both groups. Intravitreal injections...
Cervical cancer is the fourth most common in women, and it well known that high-risk human papillomavirus (hrHPV) infections are necessary carcinogenic factors for development of cervical tumors. Moreover, interaction between HPV other sexually transmitted (STIs) may increase risk progression. Self-sampling has been demonstrated to represent a valid well-accepted alternative, favoring women's participation screening programs. This study aimed investigate use FLOQSwabs® (FS) as compared two...
Sexually transmitted infections (STIs) represent a major cause of morbidity in women and men worldwide. Human Papillomavirus (HPV) are among the most prevalent STIs persistent with high-risk HPV (hrHPV) genotypes can cervical dysplasia invasive cancer. The association other infection and/or has however not yet been fully elucidated. aim this study was to assess prevalence presenting an abnormal cytology. Cervical 28 seven sexually pathogens were evaluated 177 referred for colposcopy after...
To evaluate the bactericidal activity of a diluted povidone-iodine formulation (0.6%) in comparison with most used 5% solution ophthalmic preparation.In vitro between 0.6% versus formulations, against these bacteria: Staphylococcus aureus ATCC 25923, 43300, epidermidis 12228, linezolid-resistant α99 strain, clinical isolate, Pseudomonas aeruginosa 27853, Escherichia coli 25922.About was demonstrated to be faster than preparation killing Gram-positive as well Gram-negative bacteria. Against...
The accuracy of available HPV molecular assays on self-samples needs to be evaluated as compared clinician-collected samples. This pilot study aimed investigate the BD Onclarity™ assay vaginal and first-void urine Sixty-four women referred colposcopy for cervical dysplasia performed a self-collection provided sample, after informed consent. A specimen was collected during clinician examination. All samples were tested using Viper™ LT System. Overall positive agreement (OPA) between...
Background: Human parechovirus (HPeV) and enterovirus (EV) infections are widespread can lead to a broad range of symptoms, from the common cold severe disease (SD). Because lack Italian data, this 4-year retrospective cross-sectional study aimed investigate frequency, seasonality molecular characteristics EV HPeV circulating in children younger than 5 years. Methods: A total 812 samples were collected ≤5 years (56.5% males; median age: 20.6 months; interquartile range: 30.1 months)...
HPV testing in cervical cancer screening programs offers the possibility of introducing molecular standardized biomarkers for triage HPV-positive women. This study aimed to evaluate role genotyping and viral load as possible diagnostic high-grade lesions (CIN2+) by performing a preliminary evaluation new test. Cervical specimens were obtained from 200 women referred colposcopy. Samples tested using both Anyplex™ II HR-HPV well OncoPredict HPV® Screening (SCR) quantitative typing (QT). Using...
The introduction of Human Papillomavirus (HPV) testing in cervical cancer screening enhanced the opportunity to introduce self-collection as an innovative approach improve coverage rates. Validation and standardization pre-analytical analytical procedures are crucial for quality assurance HPV tests on self-collected samples. This study evaluated performance stability vaginal samples resuspended 5 mL two non-alcohol-based media, eNat
Epigenetic biomarkers in cancer have emerged as promising tools for early detection, prognosis, and treatment response prediction. In cervical cells, hypermethylation of the host viral HPV-genome increases with severity lesions, providing a useful biomarker triage hr-HPV-positive women during treatment. The present study focuses on evaluating clinical performance FAM19A4/miR124-2 methylation test population-based screening program. Previously collected samples, after bisulfite-converted DNA,...
ABSTRACT The introduction of self‐sampling in cervical cancer screening has raised the importance HPV test validation on self‐collected samples. This study aimed to evaluate clinical accuracy OncoPredict Screening (SCR) assay vaginal and first‐void urine (FVU) samples, relative specimens, using VALHUDES Framework. FVU self‐samples followed by a clinician‐collected brushing were collected from 500 women referred colposcopy tested SCR assay. demonstrated sensitivity detect intraepithelial...
Migrant women are one of the most vulnerable population to health problems and well-being. This study aimed at implementing a counseling preventive strategy for sexually transmitted infections (STIs) in undocumented migrant Milan, Italy. Women (ages 18-65) were enrolled NAGA Centre (2012-2013) asked urine sample order carry out molecular detection Human papillomavirus (HPV), Chlamydia trachomatis (Ct), Trichomonas vaginalis (Tv), Neisseria gonorrhoeae (Ng)-DNA. Socio-demographic sexual...
Chlamydia trachomatis (Ct) and human papillomavirus (HPV) are the most prevalent sexually transmitted infections throughout world. Despite serious complications associated with chronic Ct in active women, a screening program is not yet available Italy. Moreover, HPV/Ct co-infections also known to occur frequently, increasing risk of HPV-induced carcinogenesis. The aim this study was evaluate prevalence infections, distribution serovars, incidences Ct/HPV among women recent history abnormal...