A5025696227- Heat shock proteins research
- SARS-CoV-2 and COVID-19 Research
- Cell death mechanisms and regulation
- SARS-CoV-2 detection and testing
- MicroRNA in disease regulation
- Neuroblastoma Research and Treatments
- Takotsubo Cardiomyopathy and Associated Phenomena
- Biosensors and Analytical Detection
- Cervical Cancer and HPV Research
- Probabilistic and Robust Engineering Design
- interferon and immune responses
- Cardiac electrophysiology and arrhythmias
- Virology and Viral Diseases
- FOXO transcription factor regulation
- Pancreatic and Hepatic Oncology Research
- Peptidase Inhibition and Analysis
- Cancer Risks and Factors
- Endoplasmic Reticulum Stress and Disease
- Circular RNAs in diseases
- Redox biology and oxidative stress
- Immune cells in cancer
- Enzyme Structure and Function
- Colorectal and Anal Carcinomas
- Metabolism, Diabetes, and Cancer
- Immune Cell Function and Interaction
Association for Symbolic Logic
2023-2025
University of Salerno
2008-2018
University of Bari Aldo Moro
2016
Abstract Obesity is an important risk factor for breast cancer (BC) in postmenopausal women; interlinked molecular mechanisms might be involved the pathogenesis. Increased levels of estrogens due to aromatization adipose tissue, inflammatory cytokines such as tumor necrosis factor-α, interleukin-6, and prostaglandin E2, insulin resistance hyperactivation insulin-like growth factors pathways, adipokines, oxidative stress are all abnormally regulated obese women contribute cancerogenesis....
BAG3, a member of the BAG family heat shock protein (HSP) 70 cochaperones, is expressed in response to stressful stimuli number normal cell types and constitutively variety tumors, including pancreas carcinomas, lymphocytic myeloblastic leukemias, thyroid carcinomas. Down-regulation BAG3 results death, but underlying molecular mechanisms are still elusive. Here, we investigated mechanism BAG3-dependent survival human osteosarcoma (SAOS-2) melanoma (M14) cells. We show that bag3...
The incidence and death rate of pancreatic ductal adenocarcinoma (PDAC) have increased in recent years, therefore the identification novel targets for treatment is extremely important. Interactions between cancer stromal cells are critically involved tumour formation development metastasis. Here we report that PDAC secrete BAG3, which binds activates macrophages, inducing their activation secretion supporting factors. We also identify IFITM-2 as a BAG3 receptor show it signals through PI3K...
Mammalian microRNAs (miR) regulate the expression of genes relevant for development adaptive and innate immunity against cancer. Since T cell dysfunction has previously been reported in patients with renal carcinoma (RCC; clear type), we aimed to analyze these immune cells genetic protein differences when compared normal donor freshly after isolation 35 days vitro stimulation (IVS) HLA-matched RCC tumor cells. We investigated gene profiles tumor-reactive CD8+ obtained from patient their...
Abstract Molecular mechanisms protecting cardiomyocytes from stress-induced death, including tension stress, are essential for cardiac physiology and defects in these protective can result pathological alterations. Bcl2-associated athanogene 3 (BAG3) is expressed a component of the chaperone-assisted autophagy pathway, homeostasis mechanically altered cells. BAG3 ablation mice results lethal cardiomyopathy soon after birth mutations this gene have been associated with different...
SARS-CoV-2 is constantly evolving, leading to new variants. We analysed data from 4400 SARS-CoV-2-positive samples in order pursue epidemiological variant surveillance and evaluate their impact on public health Italy the period of April-December 2021. The main circulating strain (76.2%) was Delta variant, followed by Alpha (13.3%), Omicron (5.3%), Gamma variants (2.9%). B.1.1 lineages, Eta, Beta, Iota, Mu, Kappa variants, represented around 1% cases. There were 48.2% subjects who had not...
Endometrioid endometrial cancer is the most common gynaecological tumor in developed countries, and its incidence increasing. The definition of subtypes, based on clinical endocrine features or histopathological characteristics, correlate to some extent with patient's prognosis, but there substantial heterogeneity within types. search for molecules mechanisms implied determining progression response therapy this still ongoing. BAG3 protein, a member BAG family co-chaperones, has pro-survival...
Abstract SARS-CoV-2 replication requires the synthesis of a set structural proteins expressed through discontinuous transcription ten subgenomic mRNAs (sgmRNAs). Here, we have fine-tuned droplet digital PCR (ddPCR) assays to accurately detect and quantify genomic ORF1ab sgmRNAs for nucleocapsid (N) spike (S) proteins. We analyzed 166 RNA samples from anonymized positive subjects observed recurrent characteristic pattern expression in relation total viral content. Additionally, profiles...
Background: Reliable Human Papillomavirus (HPV) testing and genotyping are essential for quality assurance in HPV-based primary screening, disease management monitoring the impact of HPV vaccination. The clinical validation molecular diagnostic assays has significantly contributed to these objectives; however, little emphasis been placed on assuring sample quality. This study aimed evaluate accuracy cellularity assessment using C-C Motif Chemokine Receptor 5 (CCR5) gene target as a marker...
Phenethyl isothiocyanate (PEITC), a cruciferous vegetable-derived compound, is versatile cancer chemopreventive agent that displays the ability to inhibit tumor growth during initiation, promotion, and progression phases in several animal models of carcinogenesis. In this report, we dissect cellular events induced by noncytotoxic concentrations PEITC human umbilical vein endothelial cells (HUVECs). early phase, treatment elicited cells' morphological changes comprise reduction cell volume...
Abstract In human cancer cells, BAG3 protein is known to sustain cell survival. Here, for the first time, we demonstrate expression of both in equine sarcoids vivo and EqS04b a sarcoid-derived fully transformed line harbouring bovine papilloma virus (BPV)-1 genome. Evidence possible involvement sarcoid carcinogenesis was obtained by immunohistochemistry analysis tumour samples. We found that most samples stained positive BAG3, even though different grade, while normal dermal fibroblasts from...
We previously identified apoptosis-induced regulator (air) as a pro-apoptotic transcript whose expression was repressed by NF-κB/Rel activity in the human leukemia cell line Jurkat (Turco, Lamberti, Bisogni, Romano, Petrella, Ammirante, Rosati, d'Avenia, Arra, Spugnini, Venuta, 2007, Leukemia 21:2557-2559). In this paper, we report that air sequence is detectable Southern blot healthy donors (HHD) leukocytes and two pancreatic adenocarcinoma lines (AsPC-1 PANC-1), providing first definitive...
Background: Reliable HPV testing and genotyping are essential for quality assurance in HPV-based primary screening, disease management monitoring the impact of vaccination. The clinical validation molecular diagnostic assays has significantly contributed to these objectives, however little emphasis been placed assuring sample quality. This study aims assess accuracy cellularity assessment using C-C Motif Chemokine Receptor 5 (CCR5) gene target as a marker adequacy diagnostics. Methods:...
Since the beginning of pandemic, SARS-CoV-2 has shown a great genomic variability, resulting in continuous emergence new variants that made their global monitoring and study priority. This work aimed to heterogeneity, temporal origin, rate viral evolution population dynamics main circulating (20E.EU1, Alpha Delta) Italy, August 2020-January 2022 period. For phylogenetic analyses, two datasets were evaluated for each variant, former comprising international genomes latter focusing on clusters...
Abstract Since the beginning of pandemic, SARS‐CoV‐2 has shown a great genomic variability, resulting in continuous emergence new variants that made their global monitoring and study priority. This work aimed to heterogeneity, temporal origin, rate viral evolution population dynamics main circulating (20E.EU1, Alpha Delta) Italy, August 2020–January 2022 period. For phylogenetic analyses, three datasets were set up, each for different lineage/variant Italy time including other Italian...
Abstract SARS-CoV-2 replication requires the synthesis of a set structural proteins expressed through discontinuous transcription ten subgenomic mRNAs (sgmRNAs). Here, we have fine-tuned droplet digital PCR (ddPCR) assays to accurately detect and quantify genomic ORF1ab sgmRNAs for nucleocapsid (N) spike (S) proteins. We analyzed 166 RNAs from anonymized COVID-19 positive subjects found recurrent characteristic pattern expression in relation total viral RNA content. Further, observed that...
Abstract Interactions between cancer cells and stromal are critically involved in tumor formation, progression development of metastasis. Here we report for the first time that human pancreatic adenocarcinoma (PDAC) secrete BAG3 binds activates macrophages, inducing their activation secretion cancer- supporting factors. We also identify IFITM-2 as a receptor, by its co-precipitation with from macrophage cell membrane impairment BAG3- induced IFITM-2- silenced macrophages. Finally show...
Abstract The aim of this study was to characterize a newly identified transcript named air (AX538681) whose genomic sequence is missing in human genome draft. as differentially expressed cDNA TNFα-stimulated Jurkat cells, transfected with IkBα-hyperexpressing vector, versus control cells (Turco et al, 2007). Indeed expression appeared be induced by pro-apoptotic stimulus and repressed NF-κB activity. Air isolated differential screening analysis an library from cells; its (3.4kb long, 79% A/T...