A5061245348- Estrogen and related hormone effects
- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA modifications and cancer
- RNA Research and Splicing
- Cancer, Lipids, and Metabolism
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Genomics and Phylogenetic Studies
- RNA regulation and disease
- Fibroblast Growth Factor Research
- Gene expression and cancer classification
- Genomics and Chromatin Dynamics
- Metabolism, Diabetes, and Cancer
- Hepatocellular Carcinoma Treatment and Prognosis
- Liver Disease Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Ubiquitin and proteasome pathways
- Circular RNAs in diseases
- Microbial Community Ecology and Physiology
- Protein Degradation and Inhibitors
- Respiratory viral infections research
- Nitric Oxide and Endothelin Effects
- Computational Drug Discovery Methods
University of Salerno
2016-2025
Center for Genomic Science
2021-2025
Ospedali Riuniti San Giovanni di Dio e Ruggi d'Aragona
2013-2025
University of Campania "Luigi Vanvitelli"
1998-2024
Genomics (United Kingdom)
2022
University of Naples Federico II
2004-2017
University of Calabria
2016
Istituti di Ricovero e Cura a Carattere Scientifico
2016
SDN Istituto di Ricerca Diagnostica e Nucleare
2016
Ceinge Biotecnologie Avanzate (Italy)
2006-2010
Despite clear evidence that exosomal microRNAs (miRNAs) are able to modulate the cellular microenvironment and RNA cargo selection is deregulated in pathological conditions, mechanisms controlling specific sorting into extracellular vesicles still poorly understood. Here, we identified binding protein SYNCRIP (synaptotagmin-binding cytoplasmic RNA-interacting protein; also known as hnRNP-Q or NSAP1) a component of hepatocyte miRNA machinery. knockdown impairs miRNAs exosomes. Furthermore,...
The main cause of morbidity and mortality in diabetes mellitus (DM) is cardiovascular complications. Diabetic cardiomyopathy (DCM) remains incompletely understood. Animal models have been crucial exploring DCM pathophysiology while identifying potential therapeutic targets. Streptozotocin (STZ) has widely used to produce experimental both type 1 2 DM (T1DM T2DM). Here, we compared these two for their effects on cardiac structure, function transcriptome. Different doses STZ diet chows were...
About 50% of cutaneous melanoma (CM) harbors the activating BRAFV600 mutation which exerts most oncogenic effects through MAPK signaling pathway. In last years, a number modulators have been identified, including Spry1. this context, we recently demonstrated that knockout Spry1 (Spry1KO) in BRAFV600-mutant CM led to cell cycle arrest and apoptosis, repressed proliferation vitro, reduced tumor growth vivo. Despite these findings, however, precise molecular mechanism linking remains be...
Journal Article Identification of an estrogen response element upstream the human c-fos gene that binds receptor and AP-1 transcription factor Get access Alessandro Weisz, Weisz * 1Instituto di Patologia Generale e Oncologia, Prima Facoltà Medicina Chirurgia, Università NapoliNaples l-80138, Italy *To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Ricardo Rosales Nucleic Acids Research, Volume 18, Issue 17, 11 September...
Transcription of hypoxia-inducible genes is regulated by hypoxia response elements (HREs) located in either the promoter or enhancer regions. Analysis these reveals presence one more binding sites for factor 1 (HIF-1). Hypoxia-inducible include vascular endothelial growth (VEGF), erythropoietin, and glycolytic enzyme genes. Site-directed mutational analysis VEGF gene revealed that an HIF-1 site (HBS) its downstream ancillary sequence (HAS) within HRE are required as cis-elements...
Estrogen stimulates DNA synthesis and cell proliferation in the luminal glandular epithelia of rodent uterus. We tested hypothesis that mitogenic effect estrogen occurs via activation expression cellular proto-oncogenes by measuring rate transcription 20 (abl, bas, erb-A, erb-B, ets, fms, fos, fps/fes, mos, myb, myc, N-myc, raf, Ha-ras, Ki-ras, N-ras, rel, sis, src, B-lym) uterus ovariectomized rats before after injection estrogen, c-onc transcriptional activity was monitored both an vitro...
// Adnan Hashim 1,* , Francesca Rizzo Giovanna Marchese 1 Maria Ravo Roberta Tarallo Giovanni Nassa Giorgio Giurato Gianluca Santamaria Angela Cordella 2 Concita Cantarella 3 and Alessandro Weisz 1,4 Laboratory of Molecular Medicine Genomics, Faculty Surgery, University Salerno, Baronissi, SA, Italy Fondazione IRCCS SDN, Napoli, Consiglio per la Ricerca e Sperimentazione in Agricoltura, Centro di l'Orticoltura, Pontecagnano, 4 Division Pathology Medical 'SS. Dio Ruggi d'Aragona' Hospital, *...
Abstract Background Estrogen receptors alpha (ERα) and beta (ERβ) are transcription factors (TFs) that mediate estrogen signaling define the hormone-responsive phenotype of breast cancer (BC). The two can be found co-expressed play specific, often opposite, roles, with ERβ being able to modulate effects ERα on gene cell proliferation. is frequently lost in BC, where its presence generally correlates a better prognosis disease. identification genomic targets BC cells thus critical step...
Aberrant activation of PI3K/AKT signalling represents one the most common molecular alterations in lung cancer, though relative contribution single components cascade to NSCLC development is still poorly defined. In this manuscript we have investigated relationship between expression and genetic pathway [KRAS, catalytic subunit PI3K (p110α), PTEN, AKT1 AKT2] AKT 107 surgically resected NSCLCs analyzed existing relationships with clinico-pathologic features. Expression analysis was performed...
Breast cancer (BC) resistance to endocrine therapy results from constitutively active or aberrant estrogen receptor α (ERα) signaling, and ways block ERα pathway in these tumors are sought after. We identified the H3K79 methyltransferase DOT1L as a novel cofactor of BC cell chromatin, where two proteins colocalize regulate target gene transcription. blockade reduces proliferation hormone-responsive cells vivo vitro, consequent cycle arrest apoptotic death, with widespread effects on...
RNA-based therapeutics highlighted novel approaches to target either coding or noncoding molecules for multiple diseases treatment. In breast cancer (BC), a multitude of deregulated long RNAs (lncRNAs) have been identified as potential therapeutic targets also in the context antiestrogen resistance, and RNA binding activity estrogen receptor α (ERα) points additional candidates interfere with estrogenic signaling. A set lncRNAs was selected among ERα-associated BC cell nuclei due their roles...
Production of nitric oxide (NO) by macrophages is enhanced upon activation bacterial endotoxins and cytokines mainly via an increase the intracellular content inducible isoform synthase (i-NOS). We have studied in detail effect several modulators macrophage activity on steady state levels i-NOS mRNA mouse macrophage-like cell line RAW 264.7. Bacterial lipopolysaccharide (LPS) interferon-gamma (IFN-gamma) were found to be effective inducers mRNA, accordance with their known ability stimulate...
Transcriptional activation of the cyclin D1 gene (CCND1) plays a pivotal role in G(1)-phase progression, which is thereby controlled by multiple regulatory factors, including nuclear receptors (NRs). Appropriate CCND1 activity essential for normal development and physiology mammary gland, where it regulated ovarian steroids through mechanism(s) that not fully elucidated. We report here promoter estrogens human breast cancer cells mediated recruitment c-Jun/c-Fos/estrogen receptor alpha...
Cytokines and bacterial lipopolysaccharides (LPSs) stimulate nitric oxide production in macrophages by inducing transcription of the gene coding for inducible isoform synthase (iNOS). We have cloned mouse iNOS promoter analysed its structural features response to interferon-γ (IFN-γ) Escherichia coli LPS RAW 264.7 macrophage-like cells. Transcription a recombinant reporter including 4 kb 5′-flanking DNA, linked chloramphenicol acetyltransferase (CAT) gene, is stimulated IFN-γ and, more...
The promoter regions of many interferon-inducible genes share a short DNA sequence motif, termed the interferon consensus (ICS) to which several regulatory proteins bind. A murine cDNA encodes an ICS binding protein has been reported (M-ICSBP). cloning human homologue ICSBP (H-ICSBP) is described. H-ICSBP shares high homology with its cognate. derived reveals restricted within first 120 amino acids three other factors, IRF-1, IRF-2, and ISGF3 gamma. Truncated lacking 33 amino-terminal fails...
Estrogen is a mitogen for the rat uterus, where it induces transient activation of c-fos and c-myc protooncogene expression, followed by increases in DNA synthesis cell proliferation. JUN-C, product c-jun protooncogene, nuclear protein that can interact with FOS to modulate activity AP-1-responsive promoters. To test whether target estrogen regulation, we measured effects 17 beta-estradiol on expression this gene uterus. A human cDNA probe detects uterus two mRNA species 2.5 3.2 kilobases....
Evidence has accumulated suggesting that the state of secondary hyperparathyroidism and elevated blood levels parathyroid hormone (PTH) in uremia participate genesis many uremic manifestations. The present study examined role PTH glucose intolerance chronic renal failure (CRF). Intravenous tolerance tests (IVGTT) euglycemic hyperglycemic clamp studies were performed dogs with CRF (NPX) without glands (NPX-PTX). There no significant differences among plasma concentrations electrolytes, degree...
Estrogens induce cell proliferation in target tissues by stimulating progression through the G1 phase of cycle. Activation cyclin D(1) gene expression is a critical feature this hormonal action. The existence rapid/nongenomic estradiol-regulated protein kinase C (PKC-alpha) and extracellular signal-regulated (ERK) signal transduction pathways, their cross talk, role played DNA synthesis transcription have been studied herein human hepatoma HepG2 cells. 17Beta-estradiol was found to rapidly...