A5004030603- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Organ Transplantation Techniques and Outcomes
- Liver Disease and Transplantation
- Organ Donation and Transplantation
- Respiratory viral infections research
- Biosensors and Analytical Detection
- COVID-19 Clinical Research Studies
- Advanced biosensing and bioanalysis techniques
- Hepatocellular Carcinoma Treatment and Prognosis
- Renal Transplantation Outcomes and Treatments
- Helicobacter pylori-related gastroenterology studies
- Virology and Viral Diseases
- COVID-19 epidemiological studies
- Liver Disease Diagnosis and Treatment
- Multiple and Secondary Primary Cancers
- Animal Virus Infections Studies
- Cancer, Lipids, and Metabolism
- Herpesvirus Infections and Treatments
- vaccines and immunoinformatics approaches
- Cancer-related molecular mechanisms research
- Transplantation: Methods and Outcomes
- Colorectal Cancer Treatments and Studies
- Nutrition and Health in Aging
- Poxvirus research and outbreaks
Ospedale Policlinico San Martino
2021-2025
Ospedale SS. Annunziata
2025
European Institute of Oncology
2022
ORCID
2021
University of Genoa
2021
Policlinico S.Orsola-Malpighi
2017-2020
University of Bologna
2017-2020
Sapienza University of Rome
1990-2004
Czech Academy of Sciences, Institute of Physiology
1992
Abstract Since May 2022, multiple human Monkeypox cases were identified in nonendemic countries, mainly among men who have sex with men. We aimed to report the features, clinical course, management, and outcome of diagnosed Dermatology Infectious Disease Units San Martino Hospital, Genoa, Italy. performed an observational study from July 1 until August 31, collecting clinical, laboratory, histological data. studied 16 Monkeypox‐infected (14 homosexual, 2 bisexual) a median age 37 years....
Abstract With the aim to explore innovative tools for organ preservation, especially in marginal organs, we hereby describe a clinical trial of ex-vivo hypothermic oxygenated perfusion (HOPE) field liver (LT) and kidney transplantation (KT) from Extended Criteria Donors (ECD) after brain death. A matched-case analysis donor recipient variables was developed: 10 HOPE-ECD livers kidneys (HOPE-L HOPE-K) were matched 1:3 with preserved static cold storage (SCS-L SCS-K). HOPE SCS groups resulted...
Long non-coding RNAs (lncRNAs) are ncRNAs more than 200 nucleotides long that participate to a wide range of biological functions. However, their role in cancer is poorly known. By using an NGS-based approach we analyzed the intragenic and poliA-lncRNAs hepatocellular carcinoma (HCC) assayed relationships between deregulated expression clinical-pathological characteristics. The profile lncRNAs was studied discovery series 28 HCC matched cirrhosis validated independent cohort 32 patients both...
The rapid and presumptive detection of SARS-CoV-2 variants may be performed using multiplex RT-PCR assays. aim this study was to evaluate the diagnostic performance five qualitative tests as compared with next-generation sequencing (NGS). We retrospectively examined a multi-variant panel (
Influenza and respiratory syncytial (RSV) viruses are expected to co-circulate with SARS-CoV-2 in the upcoming seasons clinical differential diagnosis between them is difficult. Laboratory-based RT-PCR a gold standard diagnostic method for influenza, RSV SARS-CoV-2. The objective of this study was estimate performance novel point-of-care assay STANDARD M10 Flu/RSV/SARS-CoV-2 (SD Biosensor) large number specimens diversified (co)-infection patterns viral loads. This retrospective study, which...
ABSTRACT Since early 2022 the Omicron variant has rapidly spread worldwide, becoming dominant to date. The study aimed investigate clinical and epidemiological characteristics of COVID‐19 patients reconstruct genomic epidemiology main SARS‐CoV‐2 sublineages in Italy 2022. A total 8970 samples were studied, phylogenetic analyses focused on BA.1, BA.2, BA.5 subvariants. More than half subjects received three doses vaccine experienced a reinfection. significant larger proportion unvaccinated...
In 2022–23, the human monkeypox virus (MPXV) caused a global outbreak in several non-endemic countries. Here, we evaluated diagnostic performance of four real-time qualitative PCR assays for laboratory diagnosis mpox (monkeypox) disease. From July to August 2022, 27 positive and 10 negative specimens (lesion, crust exudate swabs) were tested Hygiene Unit San Martino Hospital (Genoa, Italy) by using home-made detect MPXV generic G2R_G DNA. According manufacturer’s instructions, also...
Extraction-based real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) is currently the "gold standard" in SARS-CoV-2 diagnostics. However, some extraction-free RT-qPCR techniques have recently been developed. In this study, we compared sensitivity of traditional extraction-based, heated extraction-free, and unheated methods for detection nasopharyngeal swabs from symptomatic individuals. The method showed perfect agreement with standard extraction-based RT-qPCR....
SARS-CoV-2 is constantly evolving, leading to new variants. We analysed data from 4400 SARS-CoV-2-positive samples in order pursue epidemiological variant surveillance and evaluate their impact on public health Italy the period of April-December 2021. The main circulating strain (76.2%) was Delta variant, followed by Alpha (13.3%), Omicron (5.3%), Gamma variants (2.9%). B.1.1 lineages, Eta, Beta, Iota, Mu, Kappa variants, represented around 1% cases. There were 48.2% subjects who had not...
BACKGROUND:The persisting organ shortage in the field of transplantation recommends use marginal kidneys which poorly tolerate ischemic damage. Adenosine triphosphate (ATP) depletion during cold ischemia time (CIT) is considered crucial for graft function. We tested different strategies kidney perfusion before attempt to improve technique. MATERIAL AND METHODS:Twenty human discarded from donors after brain death and with at least 20 hours CIT were randomized following experimental groups...
Abstract The objective of the study is to evaluate 10 years down-staging strategy for liver transplantation (LT) with a median follow-up 5 years. Data on long-term results are poor and less information available hepatocellular carcinoma (HCC) non-responder patients or those ineligible down-staging. outcome 308 HCC candidates 231 LTs performed between 2003 2013 were analyzed. HCCs divided according tumor stage response therapy: 145 T2 (metering Milan Criteria, MC), 43 T3 successfully...
Background Extended criteria donors (ECD) are widely utilized due to organ shortage, but they may increase the risk of graft dysfunction and poorer outcomes. Hypothermic oxygenated perfusion (HOPE) is a recent preservation strategy for marginal kidney liver grafts, allowing redirect from anaerobic metabolism aerobic under hypothermic conditions protecting grafts oxidative species–related damage. These mechanisms improve function survival. Objective With this study, we will evaluate benefit...
The management of recurrent hepatocellular carcinoma untreatable with surgical options is based on systemic therapy sorafenib. Due to the high rates adverse events connected sorafenib, metronomic capecitabine seems a promising strategy for these patients. We analyzed data 38 patients after liver transplantation performed at our center. compared outcome 17 receiving versus 20 experiencing best supportive care and literature about treatment In group treated we observed an increased survival...
The aims of this study were to characterize new SARS-CoV-2 genomes sampled all over Italy and reconstruct the origin evolutionary dynamics in Europe between February June 2020. cluster analysis showed only small clusters including < 80 Italian isolates, while most strains intermixed whole tree. Pure observed mainly after lockdown distancing measures adopted. Lineage B B.1 spread late January early 2020, from China Veneto Lombardy, respectively. B.1.1 (20B) probably evolved within central...
Compared with reverse transcription polymerase chain reaction (RT-PCR), rapid antigen detection tests (RADTs) for SARS-CoV-2 diagnostics are faster, less expensive, but also accurate. Performance of RADTs is context-specific and depends on disease prevalence. In this real-world study, we assessed impact in an inpatient setting through the entire COVID-19 emergency phase.In matched retrospective data RT-PCR RADT laboratory diagnoses made between February 2020 May 2023 a large hospital were...
Selection of the right or left living donor kidney for transplantation is influenced by many variables. In present multi centric study including 21 Italian transplant centres, we evaluated whether centre volume surgical technique may influence selection process.Intra- and perioperative data, function, recipient graft survival were collected among 693 mini-invasive nephrectomies performed from 2002 to 2014. Centre (LOW, 1-50 cases; HIGH, >50 cases) (FULL-LAP, full laparoscopic robotic;...
Abstract Background The ongoing SARS-CoV-2 pandemic requires the availability of accurate and rapid diagnostic tests, especially in such clinical settings as emergency intensive care units. objective this study was to evaluate performance Vivalytic PCR kit lower respiratory tract (LRT) specimens. Methods Consecutive LRT specimens (bronchoalveolar lavage bronchoaspirates) were collected from Intensive Care Units San Martino Hospital (Genoa, Italy) between November 2020 January 2021. All...