Arianna Gabrieli
A5000512405- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- Respiratory viral infections research
- HIV Research and Treatment
- interferon and immune responses
- Probiotics and Fermented Foods
- PARP inhibition in cancer therapy
- vaccines and immunoinformatics approaches
- COVID-19 and healthcare impacts
- HIV/AIDS Research and Interventions
- SARS-CoV-2 detection and testing
- Gut microbiota and health
- Ubiquitin and proteasome pathways
- HIV/AIDS drug development and treatment
- Cancer-related Molecular Pathways
- Genetic Associations and Epidemiology
- Heparin-Induced Thrombocytopenia and Thrombosis
- Bioinformatics and Genomic Networks
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Parvovirus B19 Infection Studies
- Fetal and Pediatric Neurological Disorders
- Antimicrobial Peptides and Activities
- COVID-19 Impact on Reproduction
University of Milan
2020-2025
Azienda Ospedaliera Ospedale Civile di Legnano
2025
Aziende Socio Sanitarie Territoriale Ovest Milanese
2025
Luigi Sacco Hospital
2020-2024
ASST Fatebenefratelli Sacco
2021
Azienda Ospedaliero-Universitaria di Modena
2020
Istituto Ortopedico Galeazzi
2015
Istituti di Ricovero e Cura a Carattere Scientifico
2015
IFOM
2012-2013
University of Zurich
1997
Efficient therapeutic options are needed to control the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that has caused more than 922,000 fatalities as 13 September 2020. We report isolation and characterization two ultrapotent SARS-CoV-2 human neutralizing antibodies (S2E12 S2M11) protect hamsters against challenge. Cryo-electron microscopy structures show S2E12 S2M11 competitively block angiotensin-converting enzyme (ACE2) attachment also locks spike in a closed...
Abstract In December 2019, an initial cluster of interstitial bilateral pneumonia emerged in Wuhan, China. A human-to-human transmission was assumed and a previously unrecognized entity, termed coronavirus disease-19 (COVID-19) due to novel (SARS-CoV-2) described. The infection has rapidly spread out all over the world Italy been first European country experiencing endemic wave with unexpected clinical severity comparison Asian countries. It shown that SARS-CoV-2 utilizes angiotensin...
Background: Recently, loss-of-function variants in TLR7 were identified two families which COVID-19 segregates like an X-linked recessive disorder environmentally conditioned by SARS-CoV-2. We investigated whether the represent tip of iceberg a subset male patients. Methods: This is nested case-control study we compared participants with extreme phenotype selected from Italian GEN-COVID cohort SARS-CoV-2-infected (<60 y, 79 severe cases versus 77 control cases). applied LASSO Logistic...
This report describes the isolation, molecular characterization, and phylogenetic analysis of first three complete genomes severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) isolated from patients involved in outbreak COVID-19 Lombardy, Italy. Early epidemiological tracing suggests that SARS-CoV-2 was present Italy weeks before reported cases infection.
Highlights•Meis and Prep preferentially select different DNA-binding sequences in vivo•Prep shows a preference for promoters Meis promoter-remote enhancer regions•Meis associates much more than with embryonic patterning Hox target selection•TALE protein binding defines two global regulatory domains clustersSummaryThe interactions of Meis, Prep, Pbx1 TALE homeoproteins proteins are essential development disease. Although behave similarly vitro, their vivo activities remain largely unexplored....
Abstract Toll-like receptors (TLR) are crucial components in the initiation of innate immune responses to a variety pathogens, triggering production pro-inflammatory cytokines and type I II interferons, which responsible for antiviral responses. Among different TLRs, TLR7 recognizes several single-stranded RNA viruses including SARS-CoV-2. We others identified rare loss-of-function variants X-chromosomal young men with severe COVID-19 no prior history major chronic diseases, that were...
MYBBP1A is a predominantly nucleolar transcriptional regulator involved in rDNA synthesis and p53 activation via acetylation. However little further information available as to its function. Here we report that developmentally essential the mouse prior blastocyst formation. In cell culture, down-regulation of decreases growth rate wild type embryonic stem cells, embryo fibroblasts (MEFs) human HeLa where it also promotes apoptosis. cells either arrest at G2/M or undergo delayed anomalous...
The combined impact of common and rare exonic variants in COVID-19 host genetics is currently insufficiently understood. Here, from whole-exome sequencing data about 4000 SARS-CoV-2-positive individuals were used to define an interpretable machine-learning model for predicting severity. First, converted into separate sets Boolean features, depending on the absence or presence each gene. An ensemble LASSO logistic regression models was identify most informative features with respect genetic...
The aim of this study was to evaluate probiotic characteristics Lactobacillus salivarius LS01 and Bifidobacterium breve BR03 alone in combination their immunomodulatory activity asthmatic subjects. Subjects affected by allergic asthma were recruited. Initially, analyzed for growth compatibility a broth assay. To the antimicrobial strains, an agar diffusion assay performed. Finally, cytokine production peripheral blood mononuclear cells (PBMCs) stimulated with determined means specific...
ABSTRACT Since early 2022 the Omicron variant has rapidly spread worldwide, becoming dominant to date. The study aimed investigate clinical and epidemiological characteristics of COVID‐19 patients reconstruct genomic epidemiology main SARS‐CoV‐2 sublineages in Italy 2022. A total 8970 samples were studied, phylogenetic analyses focused on BA.1, BA.2, BA.5 subvariants. More than half subjects received three doses vaccine experienced a reinfection. significant larger proportion unvaccinated...
To compare SARS-CoV-2 antigen-specific antibody production and plasma neutralizing capacity against B.1 wild-type-like strain, Gamma/P.1 Delta/B.1.617.2 variants-of-concern, in subjects with different Covid-19 disease vaccination histories.Adult were: 1) Unvaccinated/hospitalized for Covid-19; 2) Covid-19-recovered followed by one BNT162b2 vaccine dose; 3) Covid-19-naïve/2-dose vaccinated. Multiplex Luminex® immunoassays measured IgG, IgA, IgM levels receptor-binding domain (RBD), spike-1...
Abstract Background Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a novel coronavirus that caused an ongoing pandemic of pathology termed Disease 19 (COVID-19). Several studies reported both COVID-19 and RTEL1 variants are associated with shorter telomere length, but direct association between the two not generally acknowledged. Here we demonstrate up to 8.6% severe patients bear ultra-rare variants, show how this subgroup can be recognized. Methods A cohort 2246...
Abstract The clinical manifestations of SARS-CoV-2 infection vary widely among patients, from asymptomatic to life-threatening. Host genetics is one the factors that contributes this variability as previously reported by COVID-19 Genetics Initiative (HGI), which identified sixteen loci associated with severity. Herein, we investigated genetic determinants mortality, performing a case-only genome-wide survival analysis, 60 days after infection, 3904 patients GEN-COVID and other European...
Lysozyme is able to lyse Gram-positive bacteria acting as muramidase on the peptidoglycan polymer. Gram-negative in vitro are not lysed by lysozyme. It was assumed that peptido-glycan protected outer membrane and thus affected lysozyme without aid of other factors such EDTA or complement which enable penetrate membrane. Accidentally, Pellegrini et al. [(1992) J. Appl. Bacteriol., 72:180-187] found per se kill some bacteria. On basis morphological immunocytochemical findings obtained from...
ABSTRACT This report describes the isolation, molecular characterization and phylogenetic analysis of first three complete genomes SARS-CoV-2 isolated from patients involved in outbreak COVID-19 Lombardy, Italy. Early epidemiological tracing suggests that was present Italy weeks before reported cases infection.
Abstract Introduction Cancer patients are frail individuals, thus the prevention of SARS‐CoV‐2 infection is essential. To date, vaccination most effective tool to prevent COVID‐19. In a previous study, we evaluated immunogenicity two doses mRNA‐based vaccines (BNT162b2 or mRNA‐1273) in solid cancer patients. We found that seroconversion rate without exposure was lower than healthy controls (66.7% vs. 95%, p = 0.0020). The present study aimed evaluate clinical efficacy same population....
Abstract Purpose Besides the well-established efficacy in preventing severe COVID-19, impact of early treatments, namely antivirals and monoclonal antibodies (mAbs), on time length to negativization SARS-CoV-2 nasal swabs is still unclear. The aim this study was compare different treatments reducing viral shedding, identifying a single drug that might potentially lead more rapid swab. Methods This single-centre, retrospective, observational conducted at Ospedale Luigi Sacco Milan. Data...
Despite its effectiveness, combination antiretroviral treatment (cART) has a limited effect on HIV DNA reservoir, which establishes early during primary infection (PHI) and is maintained by latency, homeostatic T-cells proliferation, residual replication. This can be associated with low drug exposure in lymphoid tissues and/or suboptimal adherence to drugs (ARVs). The aim of this study was assess ARV concentrations plasma, peripheral blood mononuclear cells (PBMCs) lymph nodes (LNs), their...
Abstract It is debated whether CNS involvement begins during acute human immunodeficiency virus (HIV) infection in persons without meningitis/encephalitis and specific antiretroviral drugs or combinations would be beneficial. Neurologically asymptomatic participants enrolled a randomized controlled study comparing three combination regimens (tenofovir alafenamide/emtricitabine plus dolutegravir; darunavir; both) primary HIV were enrolled. Serum CSF collected at baseline 12 48 (serum only)...
As the introduction of antiretroviral therapy (ART) during primary HIV-1 infection (PHI) could restrict establishment HIV reservoirs, we aimed to assess effect three different ART regimens on HIV-DNA load in people living with (PLWH), who started PHI. Randomized, open-label, multicentric study, including subjects PHI (defined as an incomplete Western blot and detectable plasma HIV-RNA) Italian Network Acute Infection cohort. Participants were randomly assigned (10:10:8) a fixed-dose...
We report the first case of significant fetal myocardial involvement associated with maternal SARS-CoV-2 infection, in which restoration cardiac function at birth was noted. The demonstration previous infection supported by quantification humoral response child and mother, particular presence anti-N antibodies through detection specific against BA.4/5 variant.
Abstract We employed a multifaceted computational strategy to identify the genetic factors contributing increased risk of severe COVID-19 infection from Whole Exome Sequencing (WES) dataset cohort 2000 Italian patients. coupled stratified k -fold screening, rank variants more associated with severity, training multiple supervised classifiers, predict severity based on screened features. Feature importance analysis tree-based models allowed us 16 highest support which, together age and gender...