A5013911561- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- HIV-related health complications and treatments
- Parkinson's Disease Mechanisms and Treatments
- Vitamin D Research Studies
- HIV/AIDS Research and Interventions
- Antimicrobial Resistance in Staphylococcus
- Antibiotics Pharmacokinetics and Efficacy
- Neurological disorders and treatments
- Botulinum Toxin and Related Neurological Disorders
- Cannabis and Cannabinoid Research
- COVID-19 Clinical Research Studies
- Forensic Toxicology and Drug Analysis
- SARS-CoV-2 and COVID-19 Research
- GABA and Rice Research
- SARS-CoV-2 detection and testing
- Vitamin C and Antioxidants Research
- Bipolar Disorder and Treatment
- COVID-19 Impact on Reproduction
- Neuroscience and Neuropharmacology Research
- Drug Transport and Resistance Mechanisms
- Tryptophan and brain disorders
- Opioid Use Disorder Treatment
- Diet and metabolism studies
- Pregnancy and Medication Impact
University of Turin
2020-2024
Ospedale Amedeo di Savoia
2020-2023
Ospedale San Paolo
2022
University of Milan
2022
University of Naples Federico II
2008
<h3>Objective</h3> To determine whether pain is more frequent among people with Parkinson disease (PD) than age-matched controls. <h3>Design</h3> Case-control study. <h3>Patients and Methods</h3> Logistic regression models taking into account type of pain, time between PD onset, possible confounders were used to compare 402 patients 317 healthy control subjects. <h3>Results</h3> The overall frequency was significantly greater in controls (281 [69.9%] vs 199 [62.8%];<i>P</i> = .04), mainly...
COVID-19 is characterized by an uncontrolled inflammatory response with high pro-inflammatory cytokine production through the activation of intracellular pathways, such as mitogen-activated protein kinase (MAPK). Viruses are able to exploit MAPK pathway their advantage; this relevance severe poorly described. The aim study was quantify biomarkers involved in and clarify its possible role affecting some COVID-19-related clinical features.H-RAS, C-RAF, MAPK1, MAPK2, ERK were quantified ELISA,...
Despite its effectiveness, combination antiretroviral treatment (cART) has a limited effect on HIV DNA reservoir, which establishes early during primary infection (PHI) and is maintained by latency, homeostatic T-cells proliferation, residual replication. This can be associated with low drug exposure in lymphoid tissues and/or suboptimal adherence to drugs (ARVs). The aim of this study was assess ARV concentrations plasma, peripheral blood mononuclear cells (PBMCs) lymph nodes (LNs), their...
Medical cannabis is increasingly being used in the treatment and support of several diseases syndromes. The quantitative determination active ingredients (delta-9 tetrahydrocannabinol, THC, cannabidiol, CBD) galenic oily preparations prescribed by law for each produced batch. aim this work to describe organization titration activity centralized at three regional reference laboratories Northern Italy. Pre-analytical, analytical, post-analytical phases have been defined order guarantee high...
Recently, anti-HIV treatment has achieved high efficacy and tolerability. Nevertheless, few data are available about the intracellular penetration of antiretrovirals, partly due to technical challenges related quantification. This work aimed validate an ultra-high performance liquid chromatography (UHPLC) tandem mass spectrometry (MS/MS) method for simultaneous quantification maraviroc, nevirapine, rilpivirine, dolutegravir, raltegravir, cobicistat, darunavir, ritonavir, atazanavir,...
Dalbavancin is a lipoglycopeptide approved for treatment of Gram-positive infections skin and skin-associated structures (ABSSSI). Currently, off-label use at high dosages osteoarticular deserves attention. This work aimed to study the long-term plasma pharmacokinetics dalbavancin in outpatients with ABSSSI or infections, treated either one two 1500 mg doses dalbavancin. A liquid chromatography-tandem mass spectrometry method was used measure total concentrations samples. The results were...
Dalbavancin (DBV) is an intravenous long-acting second-generation glycolipopeptide antibiotic with high efficacy and excellent tolerability, approved for use in the treatment of Gram-positive skin structure infections (ABSSSI). Nevertheless, little known about its pharmacokinetic/pharmacodynamic (PK/PD) properties real life, which also due to technical challenges quantification human plasma, preventing effective application therapeutic drug monitoring (TDM). In fact, DBV has a affinity...
Vitamin D deficiency has been associated with the severity of COVID-19. The role vitamin in pregnant women COVID-19 poorly investigated to date. aim this study was evaluate influence affecting some clinical features pregnancy between SARS-CoV-2 positive and negative patients. pathway related polymorphisms 25-hydroxyvitamin levels were quantified followed from first third trimester pregnancy. considered values ≤ 30 ng/mL. In total, 160 enrolled: 23 resulted for at least one test (molecular...
An uncontrolled inflammatory response during SARS-CoV-2 infection has been highlighted in several studies. This seems to be due pro-inflammatory cytokines whose production could regulated by vitamin D, ROS or mitogen-activated protein kinase (MAPK). Several genetic studies are present the literature concerning influences on COVID-19 characteristics, but there few data oxidative stress, MAPK and inflammation-related factors, considering gender age. Therefore, aim of this study was evaluate...
Therapeutic options to treat HIV infection have widened in the past years, improving both effectiveness and tolerability, but nucleoside reverse transcriptase inhibitors (NRTIs) are still considered standard backbone of combination regimens. drug monitoring (TDM) can be useful for these drugs, due concentration–effect relationship, with risk ineffectiveness, toxicity or adherence concerns: this scenario, robust multiplexed methods needed an effective TDM activity. In work, first validated...
Schizophrenia affects approximately 24 million people worldwide and clozapine is the most effective antipsychotic drug. Nevertheless, its use in therapy limited due to adverse effects.Therapeutic drug monitoring a clinical tool useful reduce toxicity. In literature, papers showed how psychiatric disorders could be associated with low vitamin D levels, but few studies focusing on role affecting exposure are available. A TDM repository was analyzed: levels measured liquid chromatography were...
Although studies show an annual trend for immunosuppressive drugs, particularly during different seasons, no data are available antiretroviral drugs exposures in periods of the year. For this reason, aim study was to investigate association between seasonality and plasma concentrations. Antiretroviral were measured with liquid chromatography validated methods. A total 4148 human samples analysed. Lopinavir, etravirine maraviroc levels showed seasonal fluctuation. In detail, concentrations...
Ritonavir-boosted darunavir (DRV/r) and dolutegravir (DTG) are affected by induction of metabolizing enzymes efflux transporters caused rifampicin (RIF). This complicates the treatment people living with HIV (PLWH) diagnosed tuberculosis. Recent data showed that doubling DRV/r dose did not compensate for this effect, hepatic safety was unsatisfactory. We aimed to evaluate pharmacokinetics DRV, ritonavir (RTV), DTG in presence absence RIF peripheral blood mononuclear cells (PBMCs). PLWH were...
Antiretroviral (ARV) therapy reduces inflammation and immune activation in people with HIV, but not down to the levels observed without HIV. Limited drug penetration within tissues has been argued as a potential mechanism of persistent inflammation. Data on role ARV plasma/intracellular (IC) pharmacokinetics (PK) through expression cytochrome P450 3A/membrane transporters are limited. The aim this study was investigate correlation between markers (IM) plasma/IC PK regimen HIV-positive...
Recently, large-scale screening for COVID-19 has presented a major challenge, limiting timely countermeasures. Therefore, the application of suitable rapid serological tests could provide useful information, however, little evidence regarding their robustness is currently available. In this work, we evaluated and compared analytical performance lateral-flow test (LFA) fast semiquantitative fluorescent immunoassay (FIA) anti-nucleocapsid (anti-NC) antibodies, with reverse transcriptase...
Background: Opioids are considered the cornerstone of pain management: they show good efficacy as a first-line therapy for moderate to severe cancer pain. Since pharmacokinetic/pharmacodynamic information about tissue-specific effect and toxicity opioids is still scarce, their quantification in post-mortem autoptic specimens could give interesting insights. Methods: We describe an ultra-high-performance liquid chromatography coupled with tandem mass spectrometry method simultaneous...
Background: Antiretroviral therapy reduces systemic inflammation and immune activation, but not to levels like HIV-negative. Limited drug penetration within tissues has been argued as potential mechanism of persistent inflammation. Data on the role plasma/intracellular (IC) pharmacokinetics (PK) ARV drugs through downregulation/expression cytochrome P450 3A/membrane transport proteins are limited. Aim this study was investigate correlation between markers plasma/IC PK different ARVs regimen...