Jiayi Zhou
A5000702077- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
- Hepatitis C virus research
- RNA and protein synthesis mechanisms
- Insect-Plant Interactions and Control
- Hepatitis Viruses Studies and Epidemiology
- CAR-T cell therapy research
- Hepatitis B Virus Studies
- Viral Infections and Immunology Research
- Hearing, Cochlea, Tinnitus, Genetics
- Plant Virus Research Studies
- Virus-based gene therapy research
- Viral Infections and Outbreaks Research
- Nematode management and characterization studies
- Reproductive System and Pregnancy
- SARS-CoV-2 detection and testing
- Complement system in diseases
- Environmental Toxicology and Ecotoxicology
- RNA regulation and disease
- Forensic and Genetic Research
- Mercury impact and mitigation studies
- vaccines and immunoinformatics approaches
- Migraine and Headache Studies
Shantou University
2025
Shantou University Medical College
2025
VIR Biotechnology (United States)
2020-2024
Nanjing Agricultural University
2022-2024
Guangzhou Medical University
2020-2022
Third Affiliated Hospital of Guangzhou Medical University
2020-2022
Wenzhou University
2020
Memorial University of Newfoundland
2015-2019
Obstetrics and Gynecology Hospital of Fudan University
2015
Xi’an Jiaotong-Liverpool University
2015
Efficient therapeutic options are needed to control the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that has caused more than 922,000 fatalities as 13 September 2020. We report isolation and characterization two ultrapotent SARS-CoV-2 human neutralizing antibodies (S2E12 S2M11) protect hamsters against challenge. Cryo-electron microscopy structures show S2E12 S2M11 competitively block angiotensin-converting enzyme (ACE2) attachment also locks spike in a closed...
An ideal therapeutic anti-SARS-CoV-2 antibody would resist viral escape
ABSTRACT Sotrovimab (VIR-7831) and VIR-7832 are dual action monoclonal antibodies (mAbs) targeting the spike glycoprotein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). were derived from a parent antibody (S309) isolated memory B cells 2003 (SARS-CoV) survivor. Both mAbs contain an “LS” mutation in Fc region to prolong serum half-life. In addition, encodes GAALIE that has been shown previously evoke CD8+ T-cells context vivo viral infection. neutralize wild-type variant...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron sublineages carry distinct spike mutations resulting in escape from antibodies induced by previous infection or vaccination. We show that hybrid immunity vaccine boosters elicit plasma-neutralizing against BA.1, BA.2, BA.2.12.1, and BA.4/5, breakthrough infections, but not vaccination alone, induce neutralizing the nasal mucosa. Consistent with immunological imprinting, most derived memory B cells plasma of cases...
Abstract Currently circulating SARS-CoV-2 variants have acquired convergent mutations at hot spots in the receptor-binding domain 1 (RBD) of spike protein. The effects these on viral infection and transmission efficacy vaccines therapies remains poorly understood. Here we demonstrate that recently emerged BQ.1.1 XBB.1.5 bind host ACE2 with high affinity promote membrane fusion more efficiently than earlier Omicron variants. Structures BQ.1.1, XBB.1 BN.1 RBDs bound to fragment antigen-binding...
SARS-CoV-2 entry into host cells is orchestrated by the spike (S) glycoprotein that contains an immunodominant receptor-binding domain (RBD) targeted largest fraction of neutralizing antibodies (Abs) in COVID-19 patient plasma. Little known about Abs binding to epitopes outside RBD and their contribution protection. Here, we describe 41 human monoclonal (mAbs) derived from memory B cells, which recognize S N-terminal (NTD) show a subset them neutralize ultrapotently. We define antigenic map...
SARS-CoV-2 Omicron sublineages carry distinct spike mutations and represent an antigenic shift resulting in escape from antibodies induced by previous infection or vaccination. We show that hybrid immunity vaccine boosters result potent plasma neutralizing activity against BA.1 BA.2 breakthrough infections, but not vaccination-only, induce the nasal mucosa. Consistent with immunological imprinting, most derived memory B cells of cases cross-react Wuhan-Hu-1, receptor-binding domains whereas...
Currently circulating SARS-CoV-2 variants acquired convergent mutations at receptor-binding domain (RBD) hot spots 1 . Their impact on viral infection, transmission, and efficacy of vaccines therapeutics remains poorly understood. Here, we demonstrate that recently emerged BQ.1.1. XBB.1 bind ACE2 with high affinity promote membrane fusion more efficiently than earlier Omicron variants. Structures the BQ.1.1 RBDs bound to human S309 Fab (sotrovimab parent) explain altered recognition...
Chronic hepatitis B is a global public health problem, and coinfection with delta virus (HDV) worsens disease outcome. Here, we describe (HBV) surface antigen (HBsAg)-targeting monoclonal antibody (mAb) the potential to treat chronic D.HBsAg-specific mAbs were isolated from memory cells of HBV vaccinated individuals. In vitro neutralization was determined against HDV enveloped HBsAg representing eight genotypes. Human liver-chimeric mice treated twice weekly candidate mAb starting 3 weeks...
Pregnancy is a natural process that poses an immunological challenge because non-self fetus must be accepted. During the pregnancy period, as 'allograft' inherits maternal and also paternal antigens. For successful term pregnancy, tolerated nurtured enjoying immune privileges minimize risk of being rejected by system. Multiple mechanisms contribute to tolerate semi-allogeneic fetus. Here, we summarize recent progresses on how system actively collaborates maintain balance maternal-fetal tolerance.
Herbivore-associated molecular patterns (HAMPs) enable plants to recognize herbivores and may help adjust their defense responses. Here, we report on herbivore-induced changes in a protein disulfide isomerase (PDI) widely distributed across arthropods. PDI from the spider mite Tetranychus evansi (TePDI), mesophyll-feeding agricultural pest worldwide, triggered immunity multiple Solanaceae plants. TePDI-mediated cell death Nicotiana benthamiana required plant signaling proteins SGT1...
Abstract Investigating the mechanisms of SARS-CoV-2 cellular infection is key to better understand COVID-19 immunity and pathogenesis. Infection, which involves both cell attachment membrane fusion, relies on ACE2 receptor that paradoxically found at low levels in respiratory tract, suggesting additional facilitating may exist. Here we show C-type lectin receptors, DC-SIGN, L-SIGN sialic acid-binding Ig-like 1 (SIGLEC1) function as auxiliary receptors by enhancing ACE2-mediated modulating...
Abstract Synaptic plasticity is implemented by the interaction of glutamate receptors with PDZ domain proteins. Glutamate transporters provide only known mechanism clearance from excitatory synapses, and GLT1 major transporter. We show here that interacts protein PICK1, which plays a critical role in regulating expression at synapses. A yeast two‐hybrid screen neuronal library using carboxyl tail GLT1b yielded clones expressing PICK1. The C‐terminal peptide bound to PICK1 high affinity (K i...
The recent emergence of SARS-CoV-2 variants concern (VOC) and the recurrent spillovers coronaviruses in human population highlight need for broadly neutralizing antibodies that are not affected by ongoing antigenic drift can prevent or treat future zoonotic infections. Here, we describe a monoclonal antibody (mAb), designated S2X259, recognizing highly conserved cryptic receptor-binding domain (RBD) epitope cross-reacting with spikes from all sarbecovirus clades. S2X259 neutralizes...
Abstract An ideal anti-SARS-CoV-2 antibody would resist viral escape 1–3 , have activity against diverse SARS-related coronaviruses 4–7 and be highly protective through neutralization 8–11 effector functions 12,13 . Understanding how these properties relate to each other vary across epitopes aid development of therapeutics guide vaccine design. Here, we comprehensively characterize escape, breadth, potency a panel SARS-CoV-2 antibodies targeting the receptor-binding domain (RBD), including...
An ideal anti-SARS-CoV-2 antibody would resist viral escape 1-3 , have activity against diverse SARS-related coronaviruses 4-7 and be highly protective through neutralization 8-11 effector functions 12,13 . Understanding how these properties relate to each other vary across epitopes aid development of therapeutics guide vaccine design. Here, we comprehensively characterize escape, breadth, potency a panel SARS-CoV-2 antibodies targeting the receptor-binding domain (RBD), including S309 4...
Genetic isolates provide unprecedented opportunities to identify pathogenic mutations and explore the full natural history of clinically heterogeneous phenotypes such as hearing loss. We noticed a unique audioprofile, characterized by prelingual rapid deterioration thresholds at frequencies >0.5 kHz in several adults from unrelated families island population Newfoundland. Targeted serial Sanger sequencing probands for deafness alleles (n = 23) that we previously identified this founder was...