A5046854142- HIV/AIDS drug development and treatment
- Hepatitis C virus research
- Hepatitis B Virus Studies
- HIV Research and Treatment
- Pharmacological Effects and Toxicity Studies
- Vitamin D Research Studies
- Liver Disease Diagnosis and Treatment
- Antibiotics Pharmacokinetics and Efficacy
- HIV-related health complications and treatments
- Drug Transport and Resistance Mechanisms
- Hemoglobinopathies and Related Disorders
- Antifungal resistance and susceptibility
- COVID-19 Clinical Research Studies
- HIV/AIDS Research and Interventions
- Systemic Lupus Erythematosus Research
- Biochemical and Molecular Research
- COVID-19 Impact on Reproduction
- Iron Metabolism and Disorders
- Microscopic Colitis
- Inflammatory Bowel Disease
- Chronic Lymphocytic Leukemia Research
- Analytical Methods in Pharmaceuticals
- Antibiotic Resistance in Bacteria
- Hormonal Regulation and Hypertension
- Pneumocystis jirovecii pneumonia detection and treatment
University of Turin
2016-2025
Ospedale Amedeo di Savoia
2016-2025
University of Florida
2018
Columbus Oncology and Hematology Associates
2018
GTx (United States)
2018
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes disease 2019 (COVID-19), with a clinical outcome ranging from mild to severe, including death. To date, it is unclear why some patients develop severe symptoms. Many authors have suggested the involvement of vitamin D in reducing risk infections; thus, we retrospectively investigated 25-hydroxyvitamin (25(OH)D) concentrations plasma obtained cohort Switzerland. In this cohort, significantly lower 25(OH)D levels (p = 0.004)...
Remdesivir has received significant attention for its potential application in the treatment of COVID-19, caused by SARS-CoV-2. already been tested Ebola virus disease and found to have activity against SARS MERS coronaviruses. The remdesivir core contains GS-441524, which interferes with RNA-dependent RNA polymerases alone. In non-human primates, following IV administration, is rapidly distributed into PBMCs converted within 2 h active nucleoside triphosphate form, while GS-441524...
Congenitally- or perinatally-acquired viral infections can be harmful to the fetus but data are limited about prevalence and outcomes of coronavirus disease 2019 (COVID-19) during first trimester pregnancy. We report epidemiologic from a study investigating cohort women who became pregnant just before COVID-19 pandemic. recruited 138 consecutive attending for screening (11-13 weeks gestation) at Sant'Anna Hospital, Turin, Piedmont, Italy, plateau falling phase epidemic curve. Patients were...
Therapeutic drug monitoring (TDM) for antibiotic drugs represents a consolidated practice to optimize the effectiveness and limit toxicity of specific by guiding dosage adjustments. The comparison TDM results with drug-specific pharmacokinetic/pharmacodynamic (PK/PD) parameters, based on killing dynamics bacterial susceptibility, increases probability therapeutic success.The aim this study was analytical validation new UHPLC-MS/MS assay quantification 19 antibiotics divided in two different...
The mean corpuscular volume (MCV) of peripheral blood mononuclear cells (PBMCs) was determined by Coulter Counter, and data were used to calculate the intracellular drug concentrations. A total 574 PBMC samples collected from 190 patients. MCV 282.9 fl (minimum, 207.0; maximum, 354.6), with a standard deviation 8.8%. Previous reports have often fixed value 400 for MCV, which may result in artificially low estimates concentrations antivirals.
Abstract Objectives Knowledge of the exact concentration active compounds in galenic preparations is crucial to be able ensure their quality and properly administer prescribed dose. Currently, need for titration extracts still debated. Considering this, together with absence a standard preparation method, aim this study was evaluate cannabinoids concentrations variability olive oil extracts, interlot interlaboratory extraction yield composition. Methods Two hundred one (123 (61.2%) from...
Voriconazole therapeutic drug monitoring is not consistently recommended due to its high interpatient and intrapatient variability. Here, we aimed describe our experience with voriconazole for treatment prophylaxis of invasive fungal infections in paediatric patients. A fully validated high-performance liquid chromatography-mass spectrometry method was used quantify concentration plasma, at the end dosing interval. interindividual variability shown. We enrolled 237 children, 83 receiving...
COVID-19 is characterized by an uncontrolled inflammatory response with high pro-inflammatory cytokine production through the activation of intracellular pathways, such as mitogen-activated protein kinase (MAPK). Viruses are able to exploit MAPK pathway their advantage; this relevance severe poorly described. The aim study was quantify biomarkers involved in and clarify its possible role affecting some COVID-19-related clinical features.H-RAS, C-RAF, MAPK1, MAPK2, ERK were quantified ELISA,...
Aims Arterial hypertension is an important cardiovascular risk factor. A substantial proportion of patients show resistance to antihypertensive treatment but poor adherence medication regimens also a significant cause failure. In this context, therapeutic drug monitoring (TDM) could be useful. The objective study was assess in with resistant by TDM and identify parameters that predict nonadherence. Methods Liquid chromatography tandem mass spectrometry used quantify wide panel drugs human...
High levels of reactive oxygen species (ROS) are present in people living with HIV (PLWH), produced by intense physical activity; response, our body produces antioxidant molecules. ROS influence the expression gene-encoding enzymes and transporters involved drug biotransformation. In addition, pharmacogenetics can transporter activity, thus exposure. Currently, no studies concerning this topic literature. The aim study was to investigate whether some molecules, exercise, genetic variants...
Abstract Background Vitamin D (VD) is a crucial biological agent with immunoregulatory and anti-inflammatory properties. There growing interest in identifying genes regulated by VD to explore its therapeutic potential various diseases, including Inflammatory Bowel Disease (IBD). Understanding single nucleotide polymorphisms (SNPs) genotypes of VD-associated essential for predicting outcomes biologic treatments personalizing IBD management. This study aimed evaluate the role VD-related...
The aim of this study was to assess the potential impact pharmacogenetic variability CYP2B6 and ABCB1 genes on pharmacokinetics mitotane.A retrospective analysis carried out 27 patients with adrenocortical carcinoma postoperative adjunctive mitotane. polymorphisms were genotyped tested for an association plasma trough concentration after 3, 6, 9, 12 months therapy.Patients GT/TT genotype had higher mitotane concentrations compared GG at 3 (14.80 vs. 8.01 μg/ml; P=0.008) 6 (17.70 9.75...
Therapeutic drug monitoring (TDM) of triazoles is widely used in clinical practice to optimize therapy. TDM limited by technical problems and cost considerations, such as sample storage dry-ice shipping. We aimed develop validate a new method analyse itraconazole, posaconazole voriconazole plasma spotted on dry spot devices (DSSDs) quantify them an HPLC system. Extraction from DSSDs was done using n-hexane/ethyl acetate ammonia solution. Samples were analysed with mass spectrometry...
In patients affected by chronic hepatitis because of HBV infection, long-term suppressive therapy with nucleos(t)ides analogues in the HBeAg- has shown low effects on HBsAg titre (qHBsAg) decrease, and loss is difficult to achieve. Thus, this type main goals antiviral suppression HBV-DNA ALT normalization.We retrospectively evaluated different qHBsAg kinetics 134 treatment-naïve having same characteristics: HBeAg-, infection sustained genotype D persistently undetectable HBV-DNA. Patients...
The rate of accumulation atazanavir and ritonavir within cells is still debated due to methodological limitations. Our aim was measure peripheral blood mononuclear cell (PBMC) concentrations investigate whether single-nucleotide polymorphisms OATP, ABCB1, CYP3A4 PXR genes are involved in intracellular drug penetration. HIV-positive patients administered 300 mg atazanavir/100 were enrolled. Blood sampling performed at the end dosing interval (Ctrough). PBMC-associated plasma measured by...
To compare cerebrospinal fluid (CSF) darunavir and ritonavir concentrations in patients receiving darunavir/ritonavir 800/100 mg once daily or 600/100 twice daily. determine the influence of single-nucleotide polymorphisms genes encoding for blood-brain barrier transporters (ABCB1 3435 C>T, ABCB1 1236 2677 G>T, SLCO1A2 38 A>G, 516 A>C, ABCC2 -24 G>A) on penetration into CSF.Comparative pharmacokinetics study patients.Plasma CSF (2-26 h after drug intake) were determined by a validated HPLC...
Functional variants of inosine triphosphatase (ITPA) were recently found to protect against ribavirin (RBV)-induced hemolytic anemia. However, no definitive data are yet available on the role plasma RBV concentrations hemoglobin (Hb) decrement. Moreover, have been published possible interplay between these 2 factors.A retrospective analysis included 167 patients. The ITPA rs7270101 and rs1127354 genotyped tested using χ test for association with Hb reduction at week 4. We also investigated,...