A5021320389- CRISPR and Genetic Engineering
- Cancer Genomics and Diagnostics
- Renal and related cancers
- Alzheimer's disease research and treatments
- Cancer Cells and Metastasis
- Pluripotent Stem Cells Research
- Virus-based gene therapy research
- Pancreatic and Hepatic Oncology Research
- Dementia and Cognitive Impairment Research
- Porphyrin Metabolism and Disorders
- Sirtuins and Resveratrol in Medicine
- Chronic Myeloid Leukemia Treatments
- Extracellular vesicles in disease
- Cell Adhesion Molecules Research
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Cancer Research and Treatments
- Amyotrophic Lateral Sclerosis Research
- Galectins and Cancer Biology
- Health Education and Validation
- Neurological diseases and metabolism
- Hereditary Neurological Disorders
- Skin and Cellular Biology Research
- Neonatal Health and Biochemistry
- Phytochemicals and Antioxidant Activities
Université de Bordeaux
2014-2025
Centre Hospitalier Universitaire de Bordeaux
2013-2025
Bordeaux Population Health
2012-2025
Inserm
2016-2025
Biotherapy of Genetic Diseases, Inflammatory Disorders and Cancers
2012-2024
Laboratoire de Biochimie
2020-2024
Hôpital Pellegrin
2021-2022
Nia Association
2022
ID Genomics (United States)
2022
Laboratory of Excellence GR-Ex
2020
Abstract CRISPR-Cas9 is a promising technology for genome editing. Here we use Cas9 nuclease-induced double-strand break DNA (DSB) at the UROS locus to model and correct congenital erythropoietic porphyria. We demonstrate that homology-directed repair rare compared with NHEJ pathway leading on-target indels causing unwanted dysfunctional protein. Moreover, describe unexpected chromosomal truncations resulting from only one DSB in cell lines primary cells by p53-dependent mechanism....
Abstract Background The cerebrospinal fluid (CSF) biomarkers amyloid beta 1–42, total tau, and phosphorylated tau are used increasingly for Alzheimer's disease (AD) research patient management. However, there large variations in biomarker measurements among within laboratories. Methods Data from the first nine rounds of Association quality control program was to define extent sources analytical variability. In each round, three CSF samples prepared at Clinical Neurochemistry Laboratory...
Expediting the diagnosis of pancreatic ductal adenocarcinoma (PDAC) would benefit care management, especially for start treatments requiring histological evidence. This study evaluated combined diagnostic performance circulating biomarkers obtained by peripheral and portal blood liquid biopsy in patients with resectable PDAC.Liquid biopsies were performed a prospective translational clinical trial (PANC-CTC #NCT03032913) including 22 PDAC 28 noncancer controls from February to November 2017....
Abstract CRISPR-Cas9 is a promising technology for gene therapy. However, the ON-target genotoxicity of nuclease due to DNA double-strand breaks has received little attention and probably underestimated. Here we report that genome editing targeting globin genes induces megabase-scale losses heterozygosity (LOH) from cut-site telomere (5.2 Mb). In established lines, frequent terminal chromosome 11p truncations rare copy-neutral LOH. primary hematopoietic progenitor/stem cells, detect 1.1%...
Tumor-released extracellular vesicles (EVs) contain tumor-specific cargo distinguishing them from healthy EVs, and making eligible as circulating biomarkers. Glypican 1 (GPC1)-positive exosome relevance liquid biopsy elements is still debated. We carried out a prospective study to quantify GPC1-positive exosomes in sera pancreatic ductal adenocarcinoma (PDAC) patients undergoing up-front surgery, compared controls including without cancer history displaying preneoplasic lesions. Sera were...
Abstract Iatrogenic tumorigenesis is a major limitation for the use of human induced pluripotent stem cells (hiPSCs) in hematology. The teratoma risk comes from persistence hiPSCs differentiated cell populations. Our goal was to evaluate best system purge residual before graft without compromising hematopoietic repopulation capability. Teratoma after systemic injection expressing reporter gene luciferase assessed first time. formation immune-deficient mice tracked by vivo bioimaging. We...
Abstract Introduction The current practice of quantifying cerebrospinal fluid (CSF) biomarkers as an aid in the diagnosis Alzheimer's disease (AD) varies from center to center. For a same biochemical profile, interpretation and reporting results may differ, which can lead misunderstandings raises questions about commutability tests. Methods We obtained description (pre‐)analytical protocols sample reports 40 centers worldwide. A consensus approach allowed us propose harmonized comments...
Abstract The CRISPR-Cas9 system has revolutionized our ability to precisely modify the genome and led gene editing in clinical applications. Comprehensive analysis of products at targeted cut-site revealed a complex spectrum outcomes. ON-target genotoxicity is underestimated with standard PCR-based methods necessitates appropriate more sensitive detection methods. Here, we present two complementary Fluorescence-Assisted Megabase-scale Rearrangements Detection (FAMReD) systems that enable...
The E-cadherin/beta-catenin/T-cell factor (Tcf) signaling pathway plays a crucial role in embryogenesis and carcinogenesis has recently emerged atherosclerosis. aim of this work was to investigate whether is involved smooth muscle cell proliferation induced by oxidized low-density lipoprotein (LDL). In human aortic cells, mitogenic concentration mildly LDL the activation beta-catenin, as assessed dissociation beta-catenin/cadherin complex, concomitant rise active beta-catenin cytosol....
Background This study aimed to determine the prevalence of genetic and environmental vascular risk factors in non diabetic patients with premature peripheral arterial disease, either occlusive disease or thromboangiitis obliterans, two main entities established whether some them are specifically associated one another subgroups. Methods Results included 113 (diagnosis <45-year old) presenting a (N = 64) obliterans 49), 241 controls matched for age gender. Both patient groups demonstrated...
Due to frequent mutations in certain cancers, FGFR3 gene is considered as an oncogene. However, some normal tissues, can limit cell growth and promote differentiation. Thus, action appears paradoxical.FGFR3 expression was forced pancreatic lines. The receptor exerted dual effects: it suppressed tumor epithelial-like cells had oncogenic properties mesenchymal-like cells. Distinct exclusive pathways were activated, STATs MAP Kinases Both splice variants similar effects used the same...
The "prion-like" features of Alzheimer's disease (AD) tauopathy and its relationship with amyloid-β (Aβ) have never been experimentally studied in primates phylogenetically close to humans.
Genome editing technologies create the potential for genetic studies and innovative gene therapies. Here we present new CRISPR-Cas9 tools, named ZIP editors (ZE), loaded with a single-stranded oligodeoxynucleotide (ssODN) template on Cas ribonucleoprotein complex. The ssODN is annealed to an extended guide RNA (gRNA) allowing its nuclear delivery at right place, i.e. targeted DNA cut, time. This import system easy-to-design, easy-to-use, inexpensive very versatile. It increases...
Genome editing by CRISPR-Cas9 is promising for genetic disease and cancer gene therapy. However, safety concerns are still present, particularly the ON-target genotoxicity protocols using nucleases. Quality control of edited cells before after graft mandatory, especially to assay megabase-scale genomic rearrangements induced at targeted locus. These unintended events fortunately rare but potentially deleterious. Classical PCR-based bulk approaches do not detect them or underestimate their...