A5046985587- Lipid Membrane Structure and Behavior
- Extracellular vesicles in disease
- S100 Proteins and Annexins
- Force Microscopy Techniques and Applications
- MicroRNA in disease regulation
- Nanoparticle-Based Drug Delivery
- Protein Structure and Dynamics
- Polymer Surface Interaction Studies
- Nanopore and Nanochannel Transport Studies
- Circular RNAs in diseases
- RNA Interference and Gene Delivery
- Characterization and Applications of Magnetic Nanoparticles
- Advanced Polymer Synthesis and Characterization
- ATP Synthase and ATPases Research
- Advanced biosensing and bioanalysis techniques
- Enzyme Structure and Function
- Cell Adhesion Molecules Research
- Systemic Lupus Erythematosus Research
- Viral gastroenteritis research and epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Platelet Disorders and Treatments
- Computational Drug Discovery Methods
- Clostridium difficile and Clostridium perfringens research
- RNA and protein synthesis mechanisms
- Neonatal Respiratory Health Research
Institut Polytechnique de Bordeaux
2015-2024
Centre National de la Recherche Scientifique
2015-2024
Université de Bordeaux
2015-2024
Chimie et Biologie des Membranes et des Nanoobjects
2007-2022
Institut Européen de Chimie et Biologie
2006-2022
Amsterdam UMC Location University of Amsterdam
2017
University of Amsterdam
2017
Semmelweis University
2017
Institut de Chimie
2016
Institut Universitaire de France
2015
ABSTRACT The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions extracellular vesicles (EVs), collective term covering various subtypes cell‐released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, many other names. However, specific issues arise when working with these entities, whose size amount often make them difficult to obtain as relatively...
Supported lipid bilayers (SLBs) are popular models of cell membranes with potential bio-technological applications. A qualitative understanding the process SLB formation after exposure small vesicles to a hydrophilic support is now emerging. Recent studies have revealed stunning variety effects that can take place during this self-organization process. The ensemble results in our group has unprecedented insight into intermediates SLB-formation and helped identify number parameters...
Plasma and other body fluids contain cell-derived extracellular vesicles (EVs), which participate in physiopathological processes have potential biomedical applications. In order to isolate, concentrate purify EVs, high-speed centrifugation is often used. We show here, using electron microscopy, receptor-specific gold labelling flow cytometry, that induces the formation of EV aggregates composed a mixture EVs various phenotypes morphologies. The presence made different may lead erroneous...
Hydrophobically modified maghemite (γ-Fe2O3) nanoparticles were encapsulated within the membrane of poly(trimethylene carbonate)-b-poly(l-glutamic acid) (PTMC-b-PGA) block copolymer vesicles using a nanoprecipitation process. This formation method gives simple access to highly magnetic (MNPs) (loaded up 70 wt %) together with good control over size (100−400 nm). The simultaneous loading and doxorubicin was also achieved by nanoprecipitation. deformation vesicle under an applied field has...
In this study, we report a rapid sonochemical synthesis of monodisperse nonaggregated Fe(3)O(4)@SiO(2) magnetic nanoparticles (NPs). We found that coprecipitation Fe(II) and Fe(III) in aqueous solutions under the effect power ultrasound yields smaller Fe(3)O(4) NPs with narrow size distribution (4-8 nm) compared to silent reaction. Moreover, coating silica using an alkaline hydrolysis tetraethyl orthosilicate ethanol-water mixture is accelerated many-fold presence 20 kHz ultrasonic field....
ABSTRACT Extracellular vesicles (EVs) are small, heterogeneous and difficult to measure. Flow cytometry (FC) is a key technology for the measurement of individual particles, but its application analysis EVs other submicron particles has presented many challenges produced number controversial results, in part due limitations instrument detection, lack robust methods ambiguities how data should be interpreted. These complications exacerbated by field's reporting framework, EV‐FC manuscripts...
Eukaryotic cells possess a universal repair machinery that ensures rapid resealing of plasma membrane disruptions. Before resealing, the torn is submitted to considerable tension, which functions expand disruption. Here we show annexin-A5 (AnxA5), protein self-assembles into two-dimensional (2D) arrays on membranes upon Ca2+ activation, promotes repair. Compared with wild-type mouse perivascular cells, AnxA5-null exhibit severe defect. Membrane in rescued by addition AnxA5, binds exclusively...
Extracellular vesicles (EV) in breast milk carry immune relevant proteins and could play an important role the instruction of neonatal system. To further analyze these EV to elucidate their function it is that native populations can be recovered from (stored) samples a reproducible fashion. However, impact isolation storage procedures on recovery has remained underexposed. Here, we aimed define parameters for fresh stored milk. compare various protocols across different donors, was spiked...
Adeno-associated virus (AAV) is a safe and effective vector for gene therapy retinal disorders. Gene hearing disorders not as advanced, in part because delivery to sensory hair cells of the inner ear inefficient. Although AAV transduces mouse cochlea, outer remain refractory transduction. Here, we demonstrate that vector, exosome-associated (exo-AAV), potent carrier transgenes all cells. Exo-AAV1-GFP more efficient than conventional AAV1-GFP, both cochlear explants vitro with direct...
Neural stem cell (NSC) transplantation induces recovery in animal models of central nervous system (CNS) diseases. Although the replacement lost endogenous cells was originally proposed as primary healing mechanism NSC grafts, it is now clear that transplanted NSCs operate via multiple mechanisms, including horizontal exchange therapeutic cargoes to host extracellular vesicles (EVs). EVs are membrane particles trafficking nucleic acids, proteins, metabolites and metabolic enzymes, lipids,...
Abstract Extracellular vesicles (EVs) are nanosized with a lipid bilayer that released from cells of the cardiovascular system, and considered important mediators intercellular extracellular communications. Two types EVs particular interest exosomes microvesicles, which have been identified in all tissue body fluids carry variety molecules including RNAs, proteins, lipids. potential for use diagnosis prognosis diseases as new therapeutic agents, particularly setting myocardial infarction...
The controlled fabrication of biocompatible devices made lipid bilayers deposited onto flat solid supports presents interest as models cell membranes well for their biotechnological applications. We report here on the formation supported silica nanoparticles (nanoSLBs). successive steps adsorption vesicles and nanoSLBs are revealed in detail by cryotransmission electron microscopy (cryo-EM). was achieved liposomes with positive, neutral, low net negative charge, while a high charge adsorbed...
Abstract Plasma contains cell‐derived extracellular vesicles (EVs) which participate in various physiopathological processes and have potential biomedical applications. Despite intense research activity, knowledge on EVs is limited mainly due to the difficulty of isolating characterizing sub‐micrometer particles like EVs. We recently reported that a simple flow cytometry (FCM) approach based triggering detection fluorescence signal enabled 50× more Annexin‐A5 binding (Anx5+ EVs) plasma than...