A5035159128- Cancer-related Molecular Pathways
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Xenotransplantation and immune response
- Virus-based gene therapy research
- Chronic Lymphocytic Leukemia Research
- Organ Transplantation Techniques and Outcomes
- Ethics in Clinical Research
- RNA Interference and Gene Delivery
- Heme Oxygenase-1 and Carbon Monoxide
- Biomedical Ethics and Regulation
- Genomic variations and chromosomal abnormalities
- Adenosine and Purinergic Signaling
- Glioma Diagnosis and Treatment
- Lymphoma Diagnosis and Treatment
- Cancer Treatment and Pharmacology
- Epigenetics and DNA Methylation
- Chromosomal and Genetic Variations
- SARS-CoV-2 and COVID-19 Research
- Cardiovascular Health and Disease Prevention
- Cerebrovascular and Carotid Artery Diseases
- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Pancreatic function and diabetes
- Advanced Breast Cancer Therapies
University of Milano-Bicocca
2016-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2023
Biobanking and Biomolecular Resources Research Infrastructure Consortium
2021
Institute for Experimental Endocrinology and Oncology
2020
National Research Council
2020
University of Copenhagen
2013
University of Trieste
2013
University of Milan
2003-2007
Gdańsk Medical University
2006
University of Bologna
2001-2004
A large number of hDAF transgenic pigs to be used for xenotransplantation research were generated by using sperm-mediated gene transfer (SMGT). The efficiency transgenesis obtained with SMGT was much greater than any other method. In the experiments reported, up 80% had transgene integrated into genome. Most carrying transcribed it in a stable manner (64%). great majority that expressed protein (83%). transmitted progeny. Expression and found caveolae as is human cells. functional based on...
Glioblastoma multiforme (GBM) is a grade IV astrocytoma and the most common form of malignant brain tumor in adults. GBM remains one fatal least successfully treated solid tumors: current therapies provide median survival 12-15 months after diagnosis, due to high recurrence rate. Glioma Stem Cells (GSCs) are believed be real driving force initiation, progression relapse. Therefore, better therapeutic strategies GSCs-targeted needed. Resveratrol polyphenolic phytoalexin found fruits...
Although antibody response to SARS-CoV-2 can be detected early during the infection, several outstanding questions remain addressed regarding magnitude and persistence of titer against different viral proteins their correlation with strength immune response. An ELISA assay has been developed by expressing purifying recombinant Spike Receptor Binding Domain (RBD), Soluble Ectodomain (Spike), full length Nucleocapsid protein (N). Sera from healthcare workers affected non-severe COVID-19 were...
Importance Identifying new prognostic features in colon cancer has the potential to refine histopathologic review and inform patient care. Although artificial intelligence systems have recently demonstrated significant risk stratification for several types, studies not yet shown that machine learning–derived associated with these are both interpretable usable by pathologists. Objective To evaluate whether pathologist scoring of a feature previously identified learning is survival among...
Epididymal sperm cells, incubated with plasmid DNA, showed a spontaneous tendency to interact the exogenous nucleic acid. We have investigated molecular basis of such interaction. Exogenous DNA is taken up by cells over 15- 20-min period and specifically localized on nuclear area head. was reversibly bound spermatozoa since it can be competed out excess cold competitor or other polyanions as heparin dextran sulphate. By contrast, poly-L-lysine, polycation, favours uptake. molecules large...
Recent success in clinical trials supports the use of adeno-associated viral (AAV) vectors for gene therapy retinal diseases caused by defects pigment epithelium (RPE). In contrast, evidence efficacy AAV-mediated transfer to photoreceptors, major site inherited diseases, is less robust. addition, although RPE transduction appears efficient, independently serotype used and species treated, photoreceptor has not been systematically investigated thus so far large animal models, which also may...
Abstract Purpose: Evasion from chemotherapy-induced apoptosis due to p53 loss strongly contributes drug resistance. Identification of specific targets for the treatment drug-resistant p53-null tumors would therefore increase effectiveness cancer therapy. Experimental Design: By using a kinase-directed short hairpin RNA library and HCT116p53KO colon carcinoma cells, glycogen synthase kinase 3 beta (GSK3B) was identified as target whose silencing bypasses resistance p53. cell lines with...
Bruton's tyrosine kinase (BTK) is essential for B-cell proliferation/differentiation and it generally believed that its expression function are limited to bone marrow-derived cells. Here, we report the identification characterization of p65BTK, a novel isoform abundantly expressed in colon carcinoma cell lines tumour tissue samples. p65BTK protein expressed, through heterogeneous nuclear ribonucleoprotein K (hnRNPK)-dependent internal ribosome entry site-driven translation, from transcript...
Abstract Ejaculated sperm cells from both bovine and swine animals depleted of the seminal fluid were used as vectors for transferring different plasmid DNAs into eggs during fertilization process. PCR screening about 2000 blastocysts showed that transformed embryos obtained in animal species with varying efficiencies, rate transformation depended on used. The most efficient was pSV2CAT construct, yielding 22% positive 5.7% respectively. Artificial insemination ten sows boar had been...
Abstract Transgenic animals are produced primarily by microinjecting exogenous DNA into the male pronuclei of a zygote. Microinjection is successful in mice but not efficient farm animals, limiting its general utility. We have pursued an alternative technology for producing transgenic animals: Sperm Mediated Gene Transfer (SMGT). Based on our finding that sperm cells bind and internalize DNA, we used as vector transmitting, only their own also, exogenously‐introduced gene interest to SMGT...
ABSTRACT Ischemia‐reperfusion injury, a clinical problem during cardiac surgery, involves worsened adenosine trisphosphate (ATP) generation and damage to the heart. We studied carbon monoxide (CO) pretreatment, proven valuable in rodents but not previously tested large animals, for its effects on pig hearts subjected cardiopulmonary bypass with cardioplegic arrest. Hearts of CO‐treated pigs showed significantly higher ATP phosphocreatine levels, less interstitial edema, apoptosis...
Abstract Mature sperm cells have the spontaneous capacity to take up exogenous DNA. Such DNA specifically interacts with subacrosomal segment of head corresponding nuclear area. Part sperm‐bound foreign is further internalized into nuclei. Using end‐labelled plasmid we found that 15–22% total bound associated nuclei as determined on isolated On basis autoradiographic analysis, permeability seems be a wide phenomenon involving majority In fact, DNA, incubated for different lengths time, in...
Genetic modification of cells and animals is an invaluable tool for biotechnology biomedicine. Currently, integrating vectors are used this purpose. These vectors, however, may lead to insertional mutagenesis variable transgene expression can undergo silencing. Scaffold/matrix attachment region-based nonviral systems that replicate autonomously in mammalian cells, thereby making possible safe reliable genetic higher eukaryotic organisms. In study, genetically modified pig fetuses were...
Endotoxic shock, one of the most prominent causes mortality in intensive care units, is characterized by pulmonary hypertension, systemic hypotension, heart failure, widespread endothelial activation/injury, and clotting culminating disseminated intravascular coagulation multi-organ system failure. In last few years, studies rodents have shown that administration low concentrations carbon monoxide (CO) exerts potent therapeutic effects a variety diseases/disorders. this study, we...
// Gabriele Romano 1 , Ludovica Santi Maria Rosaria Bianco 2 Rita Giuffrè Mariateresa Pettinato Cristina Bugarin 3 Garanzini Leonilde Savarese 4 Silvia Leoni Grazia Cerrito Biagio Eugenio Leone Giuseppe Gaipa Emanuela Grassilli Michele Papa Marialuisa Lavitrano Roberto Giovannoni Department of Surgery and Translational Medicine, University Milano-Bicocca, 20900, Monza, Italy c/o Mental Physical Health Preventive Second Naples, 80138, M. Tettamanti Research Center, Pediatric Clinic, Milano...
Preoperative chemoradiotherapy is widely used to improve local control of disease, sphincter preservation and survival in patients with locally advanced rectal cancer. Patients enrolled the present study underwent preoperative chemoradiotherapy, followed by surgical excision. Response was evaluated according Mandard's Tumor Regression Grade (TRG). TRG 3, 4 5 were considered as partial or no response while 1 2 complete response. From pretherapeutic biopsies 84 carcinomas available for...