A5011396320- Immune Cell Function and Interaction
- HIV Research and Treatment
- T-cell and B-cell Immunology
- RNA Research and Splicing
- Immunotherapy and Immune Responses
- HIV/AIDS drug development and treatment
- RNA regulation and disease
- Cytomegalovirus and herpesvirus research
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Immunodeficiency and Autoimmune Disorders
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- HIV/AIDS Research and Interventions
- Autoimmune and Inflammatory Disorders Research
- HIV-related health complications and treatments
- MicroRNA in disease regulation
- Hepatitis B Virus Studies
- interferon and immune responses
- NF-κB Signaling Pathways
- Bacteriophages and microbial interactions
- Reproductive System and Pregnancy
- Retinal Development and Disorders
- Financial Distress and Bankruptcy Prediction
Bambino Gesù Children's Hospital
2016-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2013-2024
Credit Suisse (Switzerland)
2022
Boston Children's Hospital
2010-2014
Telethon Institute Of Genetics And Medicine
2008-2011
University of the Andes
2001-2004
University of Milan
2003
Istituto Superiore di Sanità
2003
University of Rome Tor Vergata
1997-2003
European Institute of Oncology
1997-1999
EH is a recently identified protein–protein interaction domain found in the signal transducers Eps15 and Eps15R several other proteins of yeast nematode. We show that domains from bind vitro to peptides containing an asparagine–proline–phenylalanine (NPF) motif. Direct screening expression libraries with yielded number putative interactors, all which possessed NPF motifs were shown be responsible for interaction. Among these interactors human homolog NUMB, developmentally reguated gene...
The HBx protein of hepatitis B virus is a dual-specificity activator transcription, stimulating signal transduction pathways in the cytoplasm and transcription factors nucleus, when expressed cell lines culture. In cytoplasm, was shown to stimulate Ras-Raf-mitogen-activated kinase (MAP kinase) cascade, which essential for activation factor AP-1. Here we show that stimulates two independently regulated members MAP family transiently cells. extracellular signal-regulated kinases (ERKs) c-Jun...
The presentation of exogenous protein antigens in a major histocompatibility complex class I–restricted fashion to CD8+ T cells is called cross-presentation. We demonstrate that cross-presentation soluble viral (derived from hepatitis C virus [HCV], B [HBV], or human immunodeficiency virus) specific cell clones dramatically improved when antigen-presenting dendritic (DCs) are pulsed with the antigen presence chloroquine ammonium chloride, which reduce acidification endocytic system. export...
Natural killer (NK) cells are a major component of the host innate immune defence against various pathogens. Several viruses, including Human immunodeficiency virus 1 (HIV-1), have developed strategies to evade NK-cell response. This study was designed evaluate whether HIV-1 could interfere with expression NK cell-activating ligands, specifically human leukocyte antigen (HLA)-I-like MICA and ULBP molecules that bind NKG2D, an activating receptor expressed by all cells. Results show Nef...
Adenosine deaminases that act on dsRNA (ADARs) are enzymes target double-stranded regions of RNA converting adenosines into inosines (A-to-I editing) thus contributing to genome complexity and fine regulation gene expression. It has been described a member the ADAR family, ADAR1, can viruses affect their replication process. Here we report evidence showing ADAR1 stimulates human immuno deficiency virus type 1 (HIV-1) by using both editing-dependent editing-independent mechanisms. We show...
The human immunodeficiency virus type 1 (HIV-1) evades the immune responses of natural killer (NK) cells through mechanisms that have been partially deciphered. Here we show in HIV-1-infected T lymphocytes, early viral Nef protein downmodulates PVR (CD155, Necl-5), a ligand for activating receptor DNAM-1 (CD226) expressed by all NK cells, CD8(+) and other cell types. This novel activity is conserved proteins laboratory HIV-1 strains (NL4-3, SF2) patient-derived virus, but it not maintained...
Recent success in clinical trials supports the use of adeno-associated viral (AAV) vectors for gene therapy retinal diseases caused by defects pigment epithelium (RPE). In contrast, evidence efficacy AAV-mediated transfer to photoreceptors, major site inherited diseases, is less robust. addition, although RPE transduction appears efficient, independently serotype used and species treated, photoreceptor has not been systematically investigated thus so far large animal models, which also may...
In humans, the interaction of natural killer group 2 member D (NKG2D)-activating receptor on (NK) and CD8+ T cells with its major histocompatibility complex class I-related chain (MIC) UL16 binding protein (ULBP) ligands (NKG2DLs) promotes recognition elimination stressed cells, such as tumor or infected cells. Here, we investigated capacity HIV-1 to modulate NKG2DL expression escape NGK2D-mediated immunosurveillance. CD4+ lymphocytes, both cell surface release MICA, MICB, ULBP2 were...
Adenosine deaminases acting on RNA (ADARs) are involved in editing that converts adenosines to inosines double-stranded RNAs. ADAR1 was demonstrated be functional different viruses exerting either antiviral or proviral effects. Concerning HIV-1, several studies showed favors viral replication. The aim of this study investigate the composition ribonucleoprotein complex during HIV-1 expression. By using a dual-tag affinity purification procedure cells expressing followed by mass spectrometry...
Schimke immuno-osseous dysplasia (SIOD) is a rare multisystemic disorder with variable clinical expressivity caused by biallelic variants in SMARCAL1. A phenotype–genotype correlation has been attempted and of SMARCAL1 may be associated environmental genetic disturbances gene expression. We describe two siblings born from consanguineous parents diagnosis SIOD revealed whole exome sequencing (WES). Results: homozygous missense variant the (c.1682G>A; p.Arg561His) was identified both...
Leukocyte recirculation between blood and lymphoid tissues is required for the generation maintenance of immune responses against pathogens crucially controlled by L-selectin (CD62L) leukocyte homing receptor. CD62L has adhesion signaling functions initiates capture rolling on vascular endothelium cells entering peripheral lymph nodes. This study reveals that strongly downregulated primary CD4(+) T lymphocytes upon infection with human immunodeficiency virus type 1 (HIV-1). Reduced cell...
Viral infection may induce the cell-surface expression of PVR (CD155) that, upon recognition by its cognate activating DNAM-1 receptor present on cytotoxic lymphocytes, promote antiviral immune responses. Here we show that human immunodeficiency virus type 1 (HIV-1) Vpr protein in Jurkat T cells increases and total levels. Analysis mutated variants indicated uses same surfaces, hence probably mechanisms, to upregulate arrest cell cycle G2 phase. Moreover, found upregulation relied ability...
The adenosine deaminases acting on RNA (ADAR) enzymes catalyse conversion of to inosine in dsRNA. A positive effect ADAR1 human immunodeficiency virus type 1 (HIV-1) replication has recently been reported. Here, we show that another ADAR enzyme, ADAR2, positively affects the process HIV-1. We found that, analogously ADAR1, ADAR2 enhances release progeny virions by an editing-dependent mechanism. However, differently from does not increase infectious potential virus. Importantly,...
In the attempt of purging HIV-1 reservoir through 'shock-and-kill' strategy, it is important to select latency reversing agents (LRAs) devoid deleterious effects on antiviral function immune effector cells. Here we investigated two LRAs with PKC agonist activity, Prostratin (PRO) and Bryostatin-1 (BRY), for their impact NK cells, major effectors innate immunity whose potential in eradication has emerged recent clinical trials. Using cells healthy donors, found that exposure either PRO or BRY...
The human immunodeficiency virus type 1 (HIV-1) nef gene is a crucial determinant in AIDS disease progression. Although several vitro activities have been attributed to the Nef protein, identifying one critical for vivo pathogenicity remains elusive. In this study, we examined large number of alleles derived at various time points from 13 perinatally infected children showing different progression rates: six nonprogressors (NPs), three slow progressors (SPs), and four rapid (RPs)....
Utilizing a procedure for the purification of RNase P from Xenopus laevis germinal vesicle (GV) extracts, according to which contamination by large, cytoplasmic, cylindrical structure (1) is avoided, we demonstrate that X.laevis enzyme , like HeLa P, Is precipitated anti-Th antibodies and an RNA molecule (XL RNA), 320 nucleotides long, copurifies with activity. The sequence XL 60% homologous H1 RNA, therefore two molecules seem related.
HIV-1 preferentially infects CD4+ T cells, causing fundamental changes that eventually lead to the release of new viral particles and cell death. To investigate in detail alterations transcriptome cells upon infection, we sequenced polyadenylated RNA isolated from Jurkat infected or not with HIV-1. We found a marked global alteration gene expression following an overall trend toward induction genes, indicating widespread modification host biology. Annotation pathway analysis most deregulated...