A5002613530- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Hepatitis C virus research
- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- Cancer Immunotherapy and Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- HIV Research and Treatment
- Cytomegalovirus and herpesvirus research
- Systemic Lupus Erythematosus Research
- Diabetes and associated disorders
- Liver Diseases and Immunity
- Cytokine Signaling Pathways and Interactions
- CAR-T cell therapy research
- Neuroblastoma Research and Treatments
- Phagocytosis and Immune Regulation
- Renal Transplantation Outcomes and Treatments
- Immune Response and Inflammation
- Multiple Sclerosis Research Studies
- Cancer, Hypoxia, and Metabolism
- T-cell and Retrovirus Studies
- MicroRNA in disease regulation
- Immunodeficiency and Autoimmune Disorders
- Adenosine and Purinergic Signaling
Sapienza University of Rome
2014-2023
Istituto Pasteur
2014-2023
Italian Institute of Technology
2016-2021
Université Paris Sciences et Lettres
2020
Inserm
2020
Institut Curie
2020
Policlinico Umberto I
2001-2019
University of Campania "Luigi Vanvitelli"
2017
University of California, San Francisco
2017
University of Verona
2017
These guidelines are a consensus work of considerable number members the immunology and flow cytometry community. They provide theory key practical aspects enabling immunologists to avoid common errors that often undermine immunological data. Notably, there comprehensive sections all major immune cell types with helpful Tables detailing phenotypes in murine human cells. The latest techniques applications also described, featuring examples data can be generated and, importantly, how analysed....
The marriage between immunology and cytometry is one of the most stable productive in recent history science. A rapid search PubMed shows that, as July 2017, using "flow immunology" a term yields more than 68 000 articles, first which, interestingly, not about lymphocytes. It might be stated after short engagement, exchange wedding rings officially occurred when idea to link fluorochromes monoclonal antibodies came about. After this, recognizing different types cells became relatively easy...
CD4+CD25+Foxp3+ Tregs suppress autoimmune responses. In addition, they limit T cell responses during chronic infection, thereby minimizing cell–dependent immunopathology. We sought to investigate how are regulated in the livers of patients chronically infected with HCV, where control balance between an adequate protective immune response and suppression found that, despite accumulating proliferating at sites infection were relatively less expanded than CD4+CD25+Foxp3– effector cells. The...
Significance Recent studies have established that metabolic restrains, such as glucose restriction, impair the activities of effector T cells in tumor microenvironment. In same context, a huge expansion activated Treg tissues has been described mice and humans, contributing to suppression protective antitumor immunity. Our data demonstrate Tregs are committed survive proliferate hostile milieu thanks advantage based on combination glycolysis fatty acid synthesis oxidation. This allows...
Regulatory T (TR) cells consist of phenotypically and functionally distinct CD4+ CD8+ cell subsets engaged both in maintaining self-tolerance preventing anti–non-self effector responses (microbial, tumor, transplant, so on) that may be harmful to the host. Here we propose proinflammatory function virus-specific memory CCR7–CD8+ cells, which are massively recruited liver, inefficient (in terms IFN-γ production) patients with chronic hepatitis C virus (HCV) infection because concomitant...
Infection with hepatitis C virus (HCV), a leading cause of chronic liver diseases, can associate B lymphocyte proliferative disorders, such as mixed cryoglobulinemia and non-Hodgkin lymphoma. The major envelope protein HCV (HCV-E2) binds, high affinity CD81, tetraspanin expressed on several cell types. Here, we show that engagement CD81 human cells by combination HCV-E2 an anti-CD81 mAb triggers the JNK pathway leads to preferential proliferation naïve (CD27 - ) subset. In parallel, have...
The presentation of exogenous protein antigens in a major histocompatibility complex class I–restricted fashion to CD8+ T cells is called cross-presentation. We demonstrate that cross-presentation soluble viral (derived from hepatitis C virus [HCV], B [HBV], or human immunodeficiency virus) specific cell clones dramatically improved when antigen-presenting dendritic (DCs) are pulsed with the antigen presence chloroquine ammonium chloride, which reduce acidification endocytic system. export...
We analyzed whether normal human hepatocytes, which normally do not display Class II major histocompatibility complex antigens, can be induced to express them in vitro , and this induction has an vivo counterpart chronic liver diseases. While both α- γ-interferon expression of I only antigens on hepatocytes . Recombinant interleukin 2 had no effect antigen expression. Both could detected by indirect immunofluorescence from patients with various forms disease, regardless etiology. These...
The Ag specificity and cytotoxic function of human T cell clones, generated from lymphocytes infiltrating the liver a chronic hepatitis B patient, were studied. Both class I- II-restricted clones specifically proliferated to virus envelope proteins, but not core Ag. fine cells was studied by using rAg having different composition in relation HBV-envelope proteins or synthetic peptides preS regions. antigenic determinant recognized mapped preS2 region based on response r(preS1+preS2+S)...
Abstract Human mesenchymal stem cells (MSC) are immunosuppressive and poorly immunogenic but may act as antigen-presenting (APC) for CD4+ T-cell responses; here we have investigated their ability to serve APC in vitro CD8+ responses. MSC pulsed with peptides from viral antigens evoked interferon (IFN)-γ Granzyme B secretion specific cytotoxic T lymphocytes (CTL) were lysed, although low efficiency. transfected tumor mRNA or infected a vector carrying the Hepatitis C virus NS3Ag gene induced...
Abstract Tumor-infiltrating lymphocytes play an essential role in improving clinical outcome of neuroblastoma (NB) patients, but their relationship with other tumor-infiltrating immune cells the T cell-inflamed tumors remains poorly investigated. Here we show that dendritic (DCs) and natural killer (NK) are positively correlated T-cell infiltration human NB, both at transcriptional protein levels, associate a favorable prognosis. Multiplex imaging displays DC/NK/T cell conjugates tumor...
Mitochondria are key players in the regulation of T cell biology by dynamically responding to needs, but how these dynamics integrate cells is still poorly understood. We show here that mitochondrial pro-fission protein Drp1 fosters migration and expansion developing thymocytes both vitro vivo. In addition, we find sustains clonal cMyc-dependent metabolic reprogramming upon activation, also regulating effector numbers Migration extravasation defects exhibited Drp1-deficient mature cells,...
Chronic viral hepatitis is characterized by a dramatic lymphocyte infiltrate in the liver. Although it one of most common chronic inflammatory diseases humans, little information available on functional state these intra-hepatic lymphocytes (IHL). To address this issue, we have optimized cytofluorimetric techniques to assess directly ex vivo functions, dynamics and repertoires IHL isolated from biopsies patients with C. We estimate that 1% total body inflamed liver find that, at variance...
Abstract CTL responses against multiple hepatitis C virus (HCV) epitopes were detected in 7 of 29 (24.1%) healthy family members (HFM) persistently exposed to chronically HCV-infected patients (HCV-HFM). These precursor at very low or undetectable frequencies, as determined by limiting dilution analysis. However, when HCV-specific effector CD8+ T cells, freshly isolated from PBMC HCV-HFM, assessed a sensitive enzyme-linked immunospot assay, their frequencies severalfold higher than those...
Abstract Dendritic cells (DC) generated after a short‐term exposure of monocytes to IFN‐α and GM‐CSF (IFN‐DC) are highly effective in inducing cross‐priming CD8 + T against viral antigens. We have investigated the mechanisms responsible for special attitude these DC compared their activity with that reference DC. Antigen uptake endosomal processing capabilities were similar IFN‐DC IL‐4‐derived Both types efficiently cross‐presented soluble HCV NS3 protein specific cell clone, even though...