A5011309882- Cancer Genomics and Diagnostics
- Cancer, Hypoxia, and Metabolism
- Pancreatic and Hepatic Oncology Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Cancer Mechanisms and Therapy
- Acute Myeloid Leukemia Research
- Epigenetics and DNA Methylation
- Medical Imaging Techniques and Applications
- Colorectal Cancer Treatments and Studies
- Lung Cancer Treatments and Mutations
- Cancer-related Molecular Pathways
- RNA modifications and cancer
- Cancer therapeutics and mechanisms
- Cell death mechanisms and regulation
- Angiogenesis and VEGF in Cancer
- Nanoplatforms for cancer theranostics
- PI3K/AKT/mTOR signaling in cancer
- Cancer Treatment and Pharmacology
- Mitochondrial Function and Pathology
- 3D Printing in Biomedical Research
- Microtubule and mitosis dynamics
- Sulfur Compounds in Biology
- Radiopharmaceutical Chemistry and Applications
University of Toronto
2014-2025
Princess Margaret Cancer Centre
2015-2024
University Health Network
2014-2024
Toronto General Hospital
2012-2024
Ontario Institute for Cancer Research
2009-2020
Institució Catalana de Recerca i Estudis Avançats
2020
Heidelberg University
2020
Occupational Cancer Research Centre
2018
Mount Sinai Hospital
2004-2015
Lunenfeld-Tanenbaum Research Institute
2007-2015
A method has been developed that allows flow cytometry to be used for measuring the cellular DNA content of paraffin-embedded human tumors. Thick (i.e., 30 micron) sections were cut from tissue blocks using a microtome and dewaxed in xylene. The then rehydrated by sequentially immersing them 100, 95, 70, 50% ethanol before finally washing distilled water. Single cell suspensions prepared incubation 0.5% pepsin, pH 1.5, at 37 degrees C min. cells counted, washed, stained with 1 microgram/ml...
Purpose: To perform real-time whole genome sequencing (WGS) and RNA (RNASeq) of advanced pancreatic ductal adenocarcinoma (PDAC) to identify predictive mutational transcriptional features for better treatment selection.Experimental Design: Patients with PDAC were prospectively recruited prior first-line combination chemotherapy. Fresh tumor tissue was acquired by image-guided percutaneous core biopsy WGS RNASeq. Laser capture microdissection performed all cases. Primary endpoint feasibility...
The marriage between immunology and cytometry is one of the most stable productive in recent history science. A rapid search PubMed shows that, as July 2017, using "flow immunology" a term yields more than 68 000 articles, first which, interestingly, not about lymphocytes. It might be stated after short engagement, exchange wedding rings officially occurred when idea to link fluorochromes monoclonal antibodies came about. After this, recognizing different types cells became relatively easy...
Abstract Purpose: Anticancer drugs gain access to solid tumors via the circulatory system and must penetrate tissue kill cancer cells. Here, we study distribution of doxorubicin in relation blood vessels regions hypoxia mice. Experimental Design: The was quantified by immunofluorescence (recognized CD31) murine 16C EMT6 human prostate PC-3 xenografts. Hypoxic were identified injection EF5. Results: concentration decreases exponentially with distance from tumor vessels, decreasing half its...
By using a recently developed flow cytometric method we have analyzed cellular DNA content of paraffin-embedded histological material from cancer patients. This allows the retrospective study tumors patients whose clinical outcome is already known, and applied it to ovarian cancers, stage II breast metastatic adenocarcinoma unknown primary site. In addition knowledge patient survival, comprehensive information was available about other prognostic determinants treatment received, used...
The BRCA1/2 proteins are involved in regulation of cellular proliferation by DNA damage repair via homologous recombination. Therefore, mutation carriers with pancreatic cancer may have distinct biologic outcomes. Patients BRCA1/2-associated ductal adenocarcinoma (PDAC) diagnosed between January 1994 and December 2012 were identified from databases at three participating institutions. Clinical data collected. Disease-free survival overall (OS) analysed. Overall, 71 patients PDAC BRCA1...
Purpose The main purpose of this study was to determine the prevalence pathogenic BRCA1 and BRCA2 mutations in a consecutively ascertained clinic-based cohort patients with pancreatic ductal adenocarcinoma describe clinical family history characteristics. Patients Methods Unselected, consecutive, incident were recruited at single cancer center over 2-year period. Participants provided blood for DNA analysis history, treatment records reviewed. from all analyzed by Sanger sequencing multiplex...
There is an emerging concept that acquired genetic instability in cancer cells can arise from the dysregulation of critical DNA repair pathways due to cell stresses such as inflammation and hypoxia. Here we report hypoxia specifically down-regulates expression RAD51, a key mediator homologous recombination mammalian cells. Decreased levels Rad51 were observed multiple types during hypoxic exposure not associated with cycle profile or hypoxia-inducible factor. Analyses RAD51 gene promoter...
Standard cytotoxic treatments for neuroendocrine tumours have been associated with limited activity and remarkable toxicity. A phase II study was designed to evaluate the efficacy, safety pharmacodynamics of temsirolimus in patients advanced carcinoma (NEC). Thirty-seven progressive NEC received intravenous weekly doses 25 mg temsirolimus. Patients were evaluated tumour response, time progression (TTP), overall survival (OS) adverse events (AE). Twenty-two archival specimens, as well 13...
Hypoxia in solid tumors is associated with treatment resistance and increased metastatic potential. Although hypoxia has been reported pancreatic cancer patients, there little direct evidence that this contributes to their overall poor prognosis. To address this, we examined the associations between biological aggression a series of patient-derived xenografts grown orthotopically. Early passage were established from 16 patients undergoing surgery for maintained pancreas immune-deprived mice....
<h3>Importance</h3> Outcomes for patients with pancreatic ductal adenocarcinoma (PDAC) remain poor. Advances in next-generation sequencing provide a route to therapeutic approaches, and integrating DNA RNA analysis clinicopathologic data may be crucial step toward personalized treatment strategies this disease. <h3>Objective</h3> To classify PDAC according distinct mutational processes, explore their clinical significance. <h3>Design, Setting, Participants</h3> We performed retrospective...
Purpose Biliary cancer has a poor prognosis, and chemotherapy had little impact. The objectives of this trial were to determine the response rate, time disease progression, survival, safety profile combination gemcitabine capecitabine (GemCap) in patients with advanced biliary cancer. Patients Methods Eligible pathologically proven, locally or metastatic adenocarcinoma arising from intra- extrahepatic bile ducts gallbladder no prior chemotherapy. treated on 3-week cycle consisting at 650...
Abstract The currently available flow cytometric stains for cellular glutathione were evaluated, examining the labelling of both human and rodent cell lines under various conditions concentration, time, temperature. Procedures used that depleted, (GSH) while having a minimal effect on other sulphydryls in order to estimate linearity extent background staining. As previously reported, monochlorobimane was highly specific GSH cells but failed label adequately because its low affinity...
PURPOSE: This prospective clinical study was begun in 1994 to validate the independent prognostic impact of tumor hypoxia patients with cervix cancer treated definitive radiation therapy. PATIENTS AND METHODS: Between May and January 1999, 106 eligible epithelial had oxygen pressure (PO 2 ) measured using Eppendorf probe. Oxygenation data are presented as hypoxic proportion, defined percentage PO readings less than 5 mm/Hg (abbreviated HP median . RESULTS: The individual 48%,...