A5002976015- Gut microbiota and health
- Inflammatory Bowel Disease
- Microbial Metabolites in Food Biotechnology
- Helicobacter pylori-related gastroenterology studies
- Heat shock proteins research
- MicroRNA in disease regulation
- Digestive system and related health
- Microscopic Colitis
- Infant Nutrition and Health
- Clostridium difficile and Clostridium perfringens research
- Food composition and properties
- Nicotinic Acetylcholine Receptors Study
- Immune cells in cancer
- Cancer Mechanisms and Therapy
- Inflammasome and immune disorders
- Immunodeficiency and Autoimmune Disorders
- Cancer Genomics and Diagnostics
- Gastrointestinal motility and disorders
- Probiotics and Fermented Foods
- IL-33, ST2, and ILC Pathways
- ATP Synthase and ATPases Research
- Urinary Tract Infections Management
- Tryptophan and brain disorders
- Celiac Disease Research and Management
- Peptidase Inhibition and Analysis
University of Manitoba
2021-2025
University of Alberta
2014-2025
Children's Hospital Research Institute of Manitoba
2023-2025
Research Manitoba
2024
Children’s Health Research Institute
2024
Institute of Infection and Immunity
2023
Apotex (Canada)
2023
Ontario Institute for Cancer Research
2018-2020
Freemasons Foundation Centre for Men's Health
2015-2018
The University of Adelaide
2015-2018
Inflammatory bowel diseases (IBD) are a group of complex and multifactorial disorders with unknown etiology. Chronic intestinal inflammation develops against resident bacteria in genetically susceptible hosts. We hypothesized that host immunoglobulin (Ig) G can be used to identify involved IBD pathogenesis. IgG-bound -unbound microorganisms were collected from 32 pediatric terminal ileum aspirate washes during colonoscopy [non-IBD (n = 10), Crohn disease 15), ulcerative colitis 7)],...
<h3>Background & aims</h3> Inflammatory bowel diseases (IBD) are affected by dietary factors, including nondigestible carbohydrates (fibers), which fermented colonic microbes. Fibers overall beneficial, but not all fibers alike, and some patients with IBD report intolerance to fiber consumption. Given reproducible evidence of reduced fiber-fermenting microbes in IBD, we hypothesized that remain intact select can then bind host cell receptors, subsequently promoting gut inflammation....
Dietary fibers are plant-derived carbohydrates and associated components, that not digested within the human upper intestinal tract/gut. Traditionally they classified based on their solubility in water i.e., soluble dietary fiber (SDF) insoluble (IDF). The SDFs generally regarded as fermentable by microbiota, primarily large intestine. considered health-promoting food components have profound impacts different diseases such diabetes, cancer, cardiovascular, inflammatory bowel diseases....
Serum levels of miR-194 have been reported to predict prostate cancer recurrence after surgery, but its functional contributions this disease not studied. Herein, it is demonstrated that a driver metastasis. Prostate tissue were associated with aggressiveness and poor outcome. Ectopic delivery stimulated migration, invasion, epithelial-mesenchymal transition in human cell lines, stable overexpression enhanced metastasis intravenous intraprostatic tumor xenografts. Conversely, inhibition...
Abstract Background The cause of Crohn’s Disease (CD) remains unclear; however evidence suggests that dietary factors play a key role. With rising incidence and no definitive cure, identifying modifiable risk is critical in preventing CD development. We aimed to study the association between fermentable fibre intake future development an at-risk population. Methods Participants were recruited as part Colitis Canada GEM study, prospective cohort healthy first-degree relatives patients with...
Abstract Background The cause of Crohn’s Disease (CD) remains unclear; however evidence suggests that diet plays a key role. With rising incidence and no definitive cure, identification modifiable risk factors is critical in preventing CD development. Aims To study the association between fermentable dietary fiber intake future development an at population. Methods Participants were recruited CCC-GEM study, prospective cohort healthy first-degree relatives patients with CD. At enrolment,...
Abstract Background Interferons (IFNs) are key cytokines that protect mucosal barriers. There three types – I, II and III. Unlike I II, which pro-inflammatory, type III interferons (IFN-λs) highly expressed in the gut exert beneficial effects such as healing dampening inflammation mouse colitis models. Gut microbes can directly induce IFN-λ expression prior studies have shown microbial-derived metabolites upregulate activity lungs. However, much less is known about role of regulation gut....
Abstract Background We have been investigating the role of viral infections in development inflammatory bowel disease (IBD) genetically predisposed individuals. Using SvEv IL10 -/- model colitis, we found evidence mouse mammary tumor virus (MMTV) infection and modulated with combination antiretroviral therapy. Reducing MMTV RNA colon lowered inflammation, cytokine levels, microbial dysbiosis. Our results showed superantigen (SAg) activity, which uses to replicate lymphocytes. also exploits...
Abstract Background Epidemiologic studies have shown that increased dietary fiber intake may be associated with a reduced risk of developing Crohn’s disease (CD). Our previous research identified specific patterns serum proteomics and metabolomics CD onset. Aims We aimed to understand how fibers might biomarkers in high-risk population. Methods In the CCC-GEM Project, nested case-control cohort was established involving healthy first-degree relatives (FDRs) individuals CD. This matched 1:4...
Persistent inflammation can trigger altered epigenetic, inflammatory, and bioenergetic states. Inflammatory bowel disease (IBD) is an idiopathic characterized by chronic of the gastrointestinal tract, with evidence subsequent metabolic syndrome disorder. Studies have demonstrated that as many 42% patients ulcerative colitis (UC) who are found to high-grade dysplasia, either already had colorectal cancer (CRC) or develop it within a short time. The presence low-grade dysplasia also predictive...
Abstract The molecular chaperone Hsp90 is overexpressed in prostate cancer (PCa) and responsible for the folding, stabilization maturation of multiple oncoproteins, which are implicated PCa progression. Compared to first-in-class inhibitors such as 17-allylamino-demethoxygeldanamycin (17-AAG) that were clinically ineffective, second generation inhibitor AUY922 has greater solubility efficacy. Here, transcriptomic proteomic analyses patient-derived explants identified cytoskeletal...
Inflammatory breast cancer (IBC) is a rare and aggressive form of that associated with significantly high mortality. In spite advances in IBC diagnoses, the prognosis still poor compared to non-IBC. Due nature disease, we hypothesize elevated levels inflammatory mediators may drive tumorigenesis metastasis patients. Utilizing cell models patient tumor samples, can detect NF-κB activity hyperactivation non-canonical drivers (nuclear factor kappaB)-directed inflammation such as tyrosine...
Hsp90 is a dimeric ATPase responsible for the activation or maturation of specific set substrate proteins termed 'clients'. This molecular chaperone acts in context structurally dynamic and highly regulated cycle involving ATP, co-chaperone clients. Co-chaperone regulate conformational transitions that may be impaired mutant forms Hsp90. We report here vivo impairment commonly studied variants harbouring G313S A587T mutation are exacerbated by Hch1p. Deletion HCH1, but not AHA1, mitigates...
Background People carrying out clean intermittent self-catheterisation (CISC) to empty their bladder often suffer repeated urinary tract infections (UTIs). Continuous once-daily, low-dose antibiotic treatment (antibiotic prophylaxis) is commonly advised but knowledge of its effectiveness lacking. Objective To assess the benefit, harms and cost-effectiveness prophylaxis prevent UTIs in people who perform CISC. Design Parallel-group, open-label, patient-randomised 12-month trial allocated...
BACKGROUND While there is compelling rationale to use heat shock protein 90 (Hsp90) inhibitors for treatment of advanced prostate cancer, agents that target the N-terminal ATP-binding site Hsp90 have shown little clinical benefit. These binding induce a response activates compensatory proteins, which believed contribute in part agents' lack efficacy. Here, we describe functional characterization two novel agents, SM253 and SM258, bind N-middle linker region Hsp90, resulting reduced client...