A5063977754- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- RNA modifications and cancer
- Circular RNAs in diseases
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Phagocytosis and Immune Regulation
- Pancreatic and Hepatic Oncology Research
- RNA and protein synthesis mechanisms
- Cytokine Signaling Pathways and Interactions
- Chronic Myeloid Leukemia Treatments
- Acute Myeloid Leukemia Research
- Cancer Cells and Metastasis
- Cancer Immunotherapy and Biomarkers
- Acute Lymphoblastic Leukemia research
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- Cancer Research and Treatments
- Bioinformatics and Genomic Networks
- Gene expression and cancer classification
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Immune cells in cancer
The University of Adelaide
2016-2025
Centre for Cancer Biology
2016-2025
University of South Australia
2016-2025
South Australia Pathology
2016-2025
SA Health
2018
Children's Cancer Institute Australia
2013-2014
Kunming University of Science and Technology
2014
Matrix Research (United States)
2014
The University of Western Australia
2013
Harry Perkins Institute of Medical Research
2013
Abstract Epithelial to mesenchymal transition occurs during embryologic development allow tissue remodeling and is proposed be a key step in the metastasis of epithelial-derived tumors. The miR-200 family microRNAs plays major role specifying epithelial phenotype by preventing expression transcription repressors, ZEB1/δEF1 SIP1/ZEB2. We show here that miR-200a, miR-200b, related miR-429 are all encoded on 7.5-kb polycistronic primary miRNA (pri-miR) transcript. promoter for pri-miR located...
Epithelial-mesenchymal transition (EMT) is a form of cellular plasticity that critical for embryonic development and tumor metastasis. A double-negative feedback loop involving the miR-200 family ZEB (zinc finger E-box-binding homeobox) transcription factors has been postulated to control balance between epithelial mesenchymal states. Here we demonstrate using Madin Darby canine kidney cell line model that, although manipulation ZEB/miR-200 able repeatedly switch cells states, induction...
Metastatic disease is a primary cause of cancer-related death, and factors governing tumor cell metastasis have not been fully elucidated. Here, we address this question by using lines derived from mice that develop metastatic lung adenocarcinoma owing to expression mutant K-ras p53 . Despite having widespread somatic genetic alterations, the metastasis-prone cells retained marked plasticity. They transited reversibly between epithelial mesenchymal states, forming highly polarized spheres in...
AU-rich elements (AREs) in the 3' untranslated regions of several cytokine and oncogene mRNAs have been shown to function as signals for rapid mRNA degradation, it is assumed that many other contain sequences region are similarly targeted turnover. We used a chimeric gene composed mostly growth hormone with expression driven by c-fos promoter investigate minimal sequence required act functional destabilizing element monitor effect these on early steps degradation pathway. find neither AUUUA,...
HIF-1α is the regulated subunit of HIF-1 transcription factor, which induces a number genes involved in cellular response to hypoxia. The protein rapidly degraded cells supplied with adequate oxygen but stabilized hypoxic cells. Using polysome profile analysis, we found that translation mRNA NIH3T3 spared general reduction rate occurs during To assess whether 5′UTR contains an internal ribosome entry site (IRES), constructed dicistronic reporter inserted between two coding regions. We...
OBJECTIVE Increased deposition of extracellular matrix (ECM) within the kidney is driven by profibrotic mediators including transforming growth factor-β (TGF-β) and connective tissue factor (CTGF). We investigated whether some their effects may be mediated through changes in expression certain microRNAs (miRNAs). RESEARCH DESIGN AND METHODS Proximal tubular cells, primary rat mesangial human podocytes were analyzed for key genes, ECM proteins, miRNA after exposure to TGF-β (1–10 ng/μl)....
Epithelial tumor cells transit to a mesenchymal state in response extracellular cues, process known as epithelial-to-mesenchymal transition (EMT). The precise nature of these cues has not been fully defined, an important issue given that EMT is early event metastasis. Here, we have found population metastasis-prone mouse lung adenocarcinoma expresses Notch and ligands the ligand Jagged2 promotes Mechanistically, was promote metastasis by increasing expression GATA-binding (Gata) factors,...
The miR-200 family is a key regulator of the epithelial-mesenchymal transition, however, its role in controlling transition between cancer stem-cell-like and non-stem-cell-like phenotypes not well understood. We utilized immortalized human mammary epithelial (HMLE) cells to investigate regulation during their conversion stem-like phenotype. HMLE were found be capable spontaneous from non-stem phenotype this was accompanied by loss expression. Stem-like cell fractions isolated metastatic...
AU-rich elements (AREs) in the 3' untranslated regions of several cytokine and oncogene mRNAs have been shown to function as signals for rapid mRNA degradation, it is assumed that many other contain sequences region are similarly targeted turnover. We used a chimeric gene composed mostly growth hormone with expression driven by c-fos promoter investigate minimal sequence required act functional destabilizing element monitor effect these on early steps degradation pathway. find neither AUUUA,...
A polypeptide containing approximately equal to 112 amino acid residues, with the thymosin alpha 1 sequence at its NH2 terminus, has been isolated from rat thymus by using a radioimmunoassay an antibody prepared against synthetic 1. The new polypeptide, named "prothymosin alpha," was found be major substance crossreacting antiserum in extracts; peptides corresponding or 11 were not detected. In gel filtration pH 2.8, prothymosin emerged as single symmetrical peak apparent molecular weight of...
Cancer progression is a complex series of events thought to incorporate the reversible developmental process epithelial-to-mesenchymal transition (EMT). In vitro, microRNA-200 family maintains epithelial phenotype by posttranscriptionally inhibiting E-cadherin repressors, ZEB1 and ZEB2. Here, we used in situ hybridization immunohistochemistry assess expression miR-200 EMT biomarkers formalin-fixed paraffin-embedded human colorectal adenocarcinomas. addition, laser capture microdissection...