A5020503463- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Immunodeficiency and Autoimmune Disorders
- Systemic Lupus Erythematosus Research
- IL-33, ST2, and ILC Pathways
- Psoriasis: Treatment and Pathogenesis
- Cytokine Signaling Pathways and Interactions
- CAR-T cell therapy research
- Atherosclerosis and Cardiovascular Diseases
- Cell Adhesion Molecules Research
- Inflammatory Bowel Disease
- Tuberous Sclerosis Complex Research
- Ophthalmology and Eye Disorders
- SARS-CoV-2 and COVID-19 Research
- MicroRNA in disease regulation
- Microscopic Colitis
- COVID-19 Clinical Research Studies
- Chemokine receptors and signaling
- Extracellular vesicles in disease
- Mycobacterium research and diagnosis
- Ocular Diseases and Behçet’s Syndrome
- Multiple Sclerosis Research Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer Immunotherapy and Biomarkers
Istituto Nazionale Genetica Molecolare
2015-2024
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2019-2024
University of Padua
2024
University of Milan
1999-2024
Ospedale San Giuseppe
2022
MultiMedica
2022
Ospedale Maggiore
2019
Institute of Molecular Genetics
2014
German Rheumatism Research Centre
2007-2011
Charité - Universitätsmedizin Berlin
2007-2011
Memory T lymphocytes proliferate in vivo the absence of antigen maintaining a pool central memory cells (TCM) and effector (TEM) with distinct function homing capacity. We compared human CD4+ naive T, TCM, TEM for their capacity to response cytokines, that have been implicated cell homeostasis. Interleukin (IL)-7 IL-15 expanded very high efficiency TEM, while TCM were less responsive failed respond. Dendritic (DCs) DC-derived cytokines allowed selectively IL-4, potently boosted IL-7 by...
The marriage between immunology and cytometry is one of the most stable productive in recent history science. A rapid search PubMed shows that, as July 2017, using "flow immunology" a term yields more than 68 000 articles, first which, interestingly, not about lymphocytes. It might be stated after short engagement, exchange wedding rings officially occurred when idea to link fluorochromes monoclonal antibodies came about. After this, recognizing different types cells became relatively easy...
We previously reported that central–memory T cells (TCM cells), which express lymph node homing receptors CCR7 and CD62L, are largely devoid of effector functions but acquire characteristics effector–memory (TEM cells) (i.e., CCR7− helper [Th]1 or Th2 after stimulation with cell receptor agonists homeostatic cytokines. Here we show three chemokine identify functional subsets within the human CD4+ TCM pool. expressing CXCR3 secreted low amounts interferon γ, whereas CCR4+ produced some...
Abstract We reported that RNA condensed on protamine is protected from RNase‐mediated degradation and can be used for vaccination. Here, we show such complexes are also danger signals activate mouse cells through a MyD88‐dependent pathway. Moreover, mRNA‐protamine stimulate human blood cells. They strongly DC monocytes, leading to TNF‐α IFN‐α secretion. In addition, protamine‐RNA directly B cells, NK granulocytes. The detailed analysis of the activated cell types, study cytokines released...
Two subsets of natural and adaptive regulatory T (T reg) cells have been described, but the identity type 1 (Tr1)–like in humans is unclear. We analyzed a subset human blood CD4+ cells—CD45RA−CD25−interleukin (IL)-7 receptor (R)− cells—that rapidly secreted high levels IL-10 together with interferon γ, produced little IL-2. These IL-7R− were rare, anergic, largely Foxp3−. They expressed low Bcl-2 Ki-67 ICOS, suggesting that they recently activated vivo. Consistently, responded selectively to...
IL-10 is a prototypical anti-inflammatory cytokine, which fundamental to the maintenance of immune homeostasis, especially in intestine. There an assumption that cells producing have immunoregulatory function. However, here we report IL-10-producing CD4+ T are phenotypically and functionally heterogeneous. By combining single cell transcriptome functional analyses, identified subpopulation Foxp3neg displays regulatory activity unlike other cells, unexpectedly pro-inflammatory. The...
The aim was to investigate CD4+T-cell subsets, immune cells and their cytokine profiles in blood synovial compartments rheumatoid arthritis (RA) inflammatory osteoarthritis (OA) define specific signatures. Peripheral blood, fluid (SF) membranes (SM) of RA OA patients were analyzed. subset frequencies determined by flow cytometry, concentrations serum SF measured ELISA. In peripheral had altered regulatory T-cell higher Th17 Th1/17 than patients. the compartment patients, conventional largely...