A5035388335- Organ Transplantation Techniques and Outcomes
- Liver Disease and Transplantation
- Liver Disease Diagnosis and Treatment
- Transplantation: Methods and Outcomes
- Liver physiology and pathology
- Clinical Nutrition and Gastroenterology
- Respiratory Support and Mechanisms
- Renal Transplantation Outcomes and Treatments
- Mechanical Circulatory Support Devices
- Cardiac Arrest and Resuscitation
- Pancreatic function and diabetes
- melanin and skin pigmentation
- Cardiac Ischemia and Reperfusion
- Biochemical Analysis and Sensing Techniques
- Xenotransplantation and immune response
- MicroRNA in disease regulation
- Neonatal Respiratory Health Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Tissue Engineering and Regenerative Medicine
- Regulation of Appetite and Obesity
- Hepatitis B Virus Studies
- Antimicrobial Peptides and Activities
- Iron Metabolism and Disorders
- Muscle Physiology and Disorders
- Diet, Metabolism, and Disease
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2015-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2004-2024
Azienda Ospedaliera San Gerardo
2021-2024
Ospedale Maggiore
2008-2022
University of Milano-Bicocca
2017-2022
University of Milan
2004-2020
University of Toronto
2019
Hospital for Sick Children
2019
Novem (Netherlands)
2019
Institute of Biomedical Technologies
2019
Several studies demonstrated that mesenchymal stem cells (MSCs) reverse acute kidney injury (AKI) by a paracrine mechanism rather than MSC transdifferentiation. We recently microvesicles (MVs) released from MSCs may account for this horizontal transfer of messenger RNA and microRNA.MVs isolated were injected intravenously in rats (30 μg/rat) immediately after monolateral nephrectomy renal artery vein occlusion 45 min. To evaluate the MV effects on AKI induced ischaemia-reperfusion (IRI),...
Several studies suggested the presence of stem cells in adult normal human liver; however, a population with cell properties has not yet been isolated. The purpose present study was to identify and characterize progenitor liver. By stringent conditions liver cultures, we isolated characterized (HLSCs). HLSCs expressed mesenchymal markers CD29, CD73, CD44, CD90 but hematopoietic CD34, CD45, CD117, CD133. were also positive for vimentin nestin, marker. absence staining cytokeratin‐19, CD34...
The ventilator works mechanically on the lung parenchyma. authors set out to obtain proof of concept that ventilator-induced injury (VILI) depends mechanical power applied lung.Mechanical was defined as function transpulmonary pressure, tidal volume (TV), and respiratory rate. Three piglets were ventilated with a known be lethal (TV, 38 ml/kg; plateau 27 cm H2O; rate, 15 breaths/min). Other groups (three each) same TV per kilogram pressure but at rates 12, 9, 6, 3 breaths/min. identified...
Rationale: Unphysiologic strain (the ratio between tidal volume and functional residual capacity) stress transpulmonary pressure) can cause ventilator-induced lung damage.Objectives: To identify a strain–stress threshold (if any) above which damage occur.Methods: Twenty-nine healthy pigs were mechanically ventilated for 54 hours with producing 0.45 3.30. Ventilator-induced was defined as net increase in weight.Measurements Main Results: Initial weight capacity measured computed tomography....
Abstract Several studies indicate that adult stem cells may improve the recovery from acute tissue injury. It has been suggested they contribute to regeneration by release of paracrine factors promoting proliferation resident cells. However, involved remain unknown. In present study we found microvesicles (MVs) derived human liver (HLSC) induced in vitro and apoptosis resistance rat hepatocytes. These effects required internalization MVs hepatocytes an α 4 ‐integrin‐dependent mechanism....
Objective: Tidal volume (VT) and of gas caused by positive end-expiratory pressure (VPEEP) generate dynamic static lung strains, respectively. Our aim was to clarify whether different combinations resulting in the same large global strain, constantly produce edema. Design: Laboratory investigation. Setting: Animal unit. Subjects: Twenty-eight healthy pigs. Interventions: After computed tomography, 20 animals were ventilated for 54 hours at a strain 2.5, either entirely (VT 100% VPEEP 0%),...
Lungs behave as viscoelastic polymers. Harms of mechanical ventilation could then depend on not only amplitude (strain) but also velocity (strain rate) lung deformation. Herein, we tested this hypothesis.Laboratory investigation.Animal unit.Thirty healthy piglets.Two groups animals were ventilated for 54 hours with matched strains (ratio between tidal volume and functional residual capacity) different strain rates inspiratory time). Individual ranged 0.6 3.5 in both groups. Piglets low had...
During mechanical ventilation, stress and strain may be locally multiplied in an inhomogeneous lung. The authors investigated whether, healthy lungs, during high pressure/volume injury begins at the interface of naturally structures as visceral pleura, bronchi, vessels, alveoli. wished also to characterize nature lesions (collapse vs. consolidation).Twelve piglets were ventilated with greater than 2.5 (tidal volume/end-expiratory lung volume) until whole edema developed. At least every 3 h,...
High tidal volume can cause ventilator-induced lung injury (VILI), but positive end-expiratory pressure (PEEP) is thought to be protective. We aimed find the volumetric VILI threshold and see whether PEEP protective per se or indirectly. In 76 pigs (22 ± 2 kg), we examined lower upper limits (30.9–59.7 mL/kg) of inspiratory capacity by computed tomography (CT) scan at 45 cmH2O pressure. The underwent a 54-h mechanical ventilation with global strain ((tidal (dynamic) + (static))/functional...
Higher driving pressure during controlled mechanical ventilation is known to be associated with increased mortality in patients acute respiratory distress syndrome.Whereas syndrome are initially managed ventilation, as they improve, transitioned assisted ventilation. Whether higher assessed support (assisted) can reliably and whether worse outcomes has not been well studied.This study shows that the majority of adult syndrome, both system compliance measured ventilation.Higher significantly...
Naturally occurring variation in Membrane-bound O-acyltransferase domain-containing 7 (MBOAT7), encoding for an enzyme involved phosphatidylinositol acyl-chain remodelling, has been associated with fatty liver and hepatic disorders. Here, we examined the relationship between Mboat7 down-regulation fat accumulation.Hepatic MBOAT7 expression was surveyed 119 obese individuals experimental models. acutely silenced by antisense oligonucleotides C57Bl/6 mice, CRISPR/Cas9 HepG2 hepatocytes.In...
Positive end-expiratory pressure (PEEP) is a key element of mechanical ventilation. It should optimize recruitment, without causing excessive overdistension, but controversy exists on the best method to set it. The purpose study was test feasibility setting PEEP with electrical impedance tomography in order prevent lung de-recruitment following recruitment maneuver. We enrolled 16 patients undergoing ventilation PaO2/FiO2 <300 mmHg. In all patients, under constant tidal volume (6-8 ml/kg)...
We developed a protocol to procure lungs from uncontrolled donors after circulatory determination of death (NCT02061462). Subjects with cardiovascular collapse, treated on scene by resuscitation team and transferred the emergency room, are considered potential once declared dead. Exclusion criteria include unwitnessed no-flow period >15 min low flow >60 min. After death, lung preservation recruitment maneuvers, continuous positive airway pressure, protective mechanical ventilation is applied...
In patients with non-alcoholic fatty liver disease (NAFLD), insulin resistance (IR) associates fibrosis progression independently of the hepatic inflammation, but mechanisms are still unclear. We modeled independent contribution inflammation (non-alcoholic steatohepatitis: NASH) by exploiting methionine-choline deficient (MCD) diet, and that IR receptor (InsR) haploinsufficieny (InsR+/–) in pathogenesis C57BL/6 mice. confirmed study findings 96 NAFLD. InsR+/– enhanced fat content impaired...