A5016942680- Diabetes and associated disorders
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Pancreatic function and diabetes
- Monoclonal and Polyclonal Antibodies Research
- Diabetes Management and Research
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- Parasites and Host Interactions
- Mycobacterium research and diagnosis
- Thyroid Disorders and Treatments
- Viral gastroenteritis research and epidemiology
- Blood groups and transfusion
- Galectins and Cancer Biology
- Cell Adhesion Molecules Research
- Toxin Mechanisms and Immunotoxins
- Research on Leishmaniasis Studies
- Erythrocyte Function and Pathophysiology
- RNA Interference and Gene Delivery
- Adrenal Hormones and Disorders
- Atherosclerosis and Cardiovascular Diseases
- Macrophage Migration Inhibitory Factor
- Artificial Immune Systems Applications
- Inflammatory Bowel Disease
Dublin City University
2025
New York Proton Center
2015-2024
Science Oxford
2015-2024
University College London
2015-2024
University of Cambridge
2011-2021
Schlumberger (British Virgin Islands)
2021
University of Illinois Urbana-Champaign
2018
University of North Carolina at Chapel Hill
2018
Duke University
2018
Oxfam
2018
These guidelines are a consensus work of considerable number members the immunology and flow cytometry community. They provide theory key practical aspects enabling immunologists to avoid common errors that often undermine immunological data. Notably, there comprehensive sections all major immune cell types with helpful Tables detailing phenotypes in murine human cells. The latest techniques applications also described, featuring examples data can be generated and, importantly, how analysed....
Th17 cells are involved in the pathogenesis of many autoimmune diseases, but it is not clear whether they play a pathogenic role type 1 diabetes. Here we investigated mouse with specificity for an islet antigen can induce diabetes upon transfer into NOD/SCID recipient mice. Induction mice via adoptive Th1 from BDC2.5 transgenic was prevented by treatment neutralizing IFN-γ-specific antibody. This result suggested major induction disease this model Nevertheless, highly purified caused...
The marriage between immunology and cytometry is one of the most stable productive in recent history science. A rapid search PubMed shows that, as July 2017, using "flow immunology" a term yields more than 68 000 articles, first which, interestingly, not about lymphocytes. It might be stated after short engagement, exchange wedding rings officially occurred when idea to link fluorochromes monoclonal antibodies came about. After this, recognizing different types cells became relatively easy...
SUMMARY The spot-ELISA technique has been used to enumerate the frequency of cells secreting tumor necrosis factor-alpha (TNF-α) and interferon-γ (IFN-γ), isolated from biopsies normal intestine children with inflammatory bowel disease. TNF-α production was undetectable in six out 12 other it ranged 60 580 TNF-α-secreting cells/106 intestinal cells. In contrast, Crohn's disease (n= 9) all showed elevated frequencies TNF-á-secreting (500–12 000 cells). ulcerative colitis, four eight had...
Abstract Infection with Schistosoma mansoni ( S. ) or exposure to eggs from this helminth inhibits the development of type 1 diabetes in NOD mice. In study we show that soluble extracts worm egg completely prevent onset these mice but only if injection is started at 4 weeks age. T cells diabetes‐protected make IL‐10 recall responses parasite antigens. These are furthermore impaired their ability transfer NOD‐SCID recipients. Bone marrow dendritic derived found more and less IL‐12 following...
The spontaneous development of insulin dependent diabetes mellitus in non‐obese diabetic (NOD) mice has been shown to be mediated by a Th1 response against beta cell antigens. It is known that murine models Schistosoma mansoni infection, egg production associated with switch from Th2 response. This subsequent dominance S.mansoni infected influence the other infectious agents or We therefore determined whether infection could incidence (IDDM) NOD mice. Infection this helminth significantly...
Gastrointestinal nematode infections are prevalent worldwide and potent inducers of T helper 2 responses with the capacity to modulate immune response heterologous antigens. Parasitic helminth infection has even been shown associated autoimmune diseases. Nonobese diabetic (NOD) mice provide a model for studying human diabetes; as in humans, development diabetes NOD linked loss self-tolerance beta cell autoantigens. Previous studies mouse have that bacterial appears inhibit type 1 by...
Abstract Regulatory T cells (Tregs) have been implicated as key players in immune tolerance well suppression of antitumor responses. The chemotherapeutic alkylating agent cyclophosphamide (CY) is widely used the treatment tumors and some autoimmune conditions. Although previous data has demonstrated that Tregs may be preferentially affected by CY, its relevance promoting conditions not addressed. nonobese diabetic mouse spontaneously develops type-1 diabetes (T1D). We demonstrate this study...
Schistosoma mansoni soluble egg antigens (SEA) profoundly regulate the infected host's immune system. We previously showed that SEA prevents type 1 diabetes in NOD mice and splenocytes from SEA-treated have reduced ability to transfer NOD.scid recipients. To further characterize mechanism of prevention we examined cell types involved CD25(+) T-cell depletion donors restored their diabetes. Furthermore, treatment increased number proportional representation Foxp3(+) T cells pancreas mice....
CD4+ T cell lines were generated from the spleens of diabetic NOD mice against crude membrane preparations derived a rat insulinoma. Adoptive transfer these into neonatal confirms that overt diabetes is induced by gamma-IFN-secreting Th1 cells, whereas IL-4-secreting Th2 cells resulted in nondestructive peri-islet insulitis. Analysis antigens recognized individual clones line included reactivity an insulinoma fraction enriched proteins approximately 38 kD. Immune responses to same antigen...
Abstract Insulin‐dependent diabetes mellitus can be transferred into young irradiated non‐obese diabetic (NOD) mice by spleen cells from a NOD donor. T (both L3T4 + and Ly‐2 ) enter the pancreas 2 weeks following transfer. They are present initially at peri‐islet locations but progressively infiltrate islet with accompanying β cell destruction. The is heterogeneous respect to V usage. Inflammatory macrophages (Mac‐1 , F4/80 detected 1 week after transfer continue recruited during disease...
Use of monoclonal antibodies directed against rat macrophages and serial pancreatic biopsy in the prediabetic period have enabled us to document involvement events leading onset diabetes spontaneously diabetic BB/E rat. A few weeks before disease, there is marked recruitment accumulation ED1+ at periductal perivascular locations adjacent noninfiltrated islets. These recruited cells, distinct from resident ED2+ tissue macrophages, then infiltrate Infiltration pancreas by ED1 + therefore a...
Type 1 diabetes development in NOD mice appears to require both CD4+ and CD8 + T cells.However, there are some situations where it has been suggested that either or CD8+ cells able mediate the absence of other population.In case transgenic mice, this may reflect numbers antigen-specific access pancreas recruit cell types such as macrophages leading a release high concentrations damaging cytokines.Previous studies examining requirement for have used antibodies specific CD8α.It is known CD8α...