A5043364236- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Allergic Rhinitis and Sensitization
- IL-33, ST2, and ILC Pathways
- Immune cells in cancer
- Asthma and respiratory diseases
- Chemokine receptors and signaling
- Food Allergy and Anaphylaxis Research
- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Immune Response and Inflammation
- Receptor Mechanisms and Signaling
- Helicobacter pylori-related gastroenterology studies
- Urban Development and Societal Issues
- Blood Coagulation and Thrombosis Mechanisms
- Neonatal Respiratory Health Research
- Academic Research in Diverse Fields
- Environmental Sustainability and Education
- Synthesis and Biological Evaluation
- Web Data Mining and Analysis
- Water Quality and Pollution Assessment
- Geography and Environmental Studies
- Autophagy in Disease and Therapy
University of Lübeck
2016-2025
University Hospital Schleswig-Holstein
2024-2025
Universidade Federal de Goiás
2022
Universidade Federal do Rio de Janeiro
2014-2017
Albert Einstein College of Medicine
2012
Hospital Universitario de Gran Canaria Doctor Negrín
2004
These guidelines are a consensus work of considerable number members the immunology and flow cytometry community. They provide theory key practical aspects enabling immunologists to avoid common errors that often undermine immunological data. Notably, there comprehensive sections all major immune cell types with helpful Tables detailing phenotypes in murine human cells. The latest techniques applications also described, featuring examples data can be generated and, importantly, how analysed....
B cells play a central role in humoral immunity but also have antibody-independent functions. Studies to date focused on blood and secondary lymphoid organs whether reside non-lymphoid (NLO) homeostasis is unknown. Here we identify, using intravenous labeling parabiosis, bona-fide tissue-resident cell population lung, liver, kidney urinary bladder, substantial proportion of which are B-1a cells. Tissue-resident present neonatal tissues germ-free mice NLOs, albeit lower numbers than specific...
A hallmark of aging is an imbalance between production and clearance reactive oxygen species increased levels oxidatively damaged biomolecules. Herein, we demonstrate that splenic nodal antigen-presenting cells purified from mice accumulate modified proteins with side-chain carbonylation, advanced glycation end products, lipid peroxidation. Furthermore, show the endosomal accumulation interferes efficient processing exogenous antigens degradation macroautophagy-delivered proteins. In support...
Abstract C3a exerts multiple biologic functions through activation of its cognate receptor. C3−/− and C3aR−/− mice have been instrumental in defining important roles the C3a/C3aR axis regulation acute chronic inflammatory diseases, including ischemia/reperfusion injury, allergic asthma, autoimmune nephritis, rheumatoid arthritis. Surprisingly little is known about C3aR expression function immune stromal cells. To close this gap, we generated a floxed tandem-dye Tomato (tdTomato)–C3aR...
Background: Mustard IgE‐mediated allergy is supposed to be a rare cause of food allergy, and its clinical features cross‐reactivities have not been fully elucidated. Methods: A prospective study was carried out, recruiting mustard allergic patients, paired control subjects. questionnaire administered, skin‐prick tests (SPT) with panels aeroallergens foods, serum extraction for in vitro double‐blind placebo‐controlled challenges (DBPCFC) were performed. Results: Thirty‐eight mainly adult...
Abstract The biological significance of C5a receptor [(C5aR)2/C5L2], a seven-transmembrane binding and C5adesArg, remains ill-defined. Specific ligation C5aR2 inhibits C5a-induced ERK1/2 activation, strengthening the view that regulates C5aR1-mediated effector functions. Although C5aR1 are often coexpressed, detailed picture expression in murine cells tissues is still lacking. To close this gap, we generated floxed tandem dye (td)Tomato–C5aR2 knock-in mouse used to track tissue-residing...
Immunoglobulin E (IgE) is a major mediator of allergic reactions and can activate mast cells basophils via the high-affinity IgE receptor. Some IgE-mediated allergies last lifetime, but IgE-secreting plasma (PCs) IgE+ memory B (MBCs) are rare, leading to questions about origin allergen-specific antibodies (Abs). In recent years, studies have suggested that cell predominantly held by IgG(1) MBCs, which become PCs during recall response [1, 2]. A article Koenig colleagues proposed novel...
<title>Abstract</title> Secretory IgA (SIgA) is critical for maintaining the intestinal barrier. A dysregulated B-cell compartment and altered Ig secretion have been well documented in Crohn’s disease (CD) patients, although their origin unknown. To unravel role of mucosal humoral immunity CD pathogenesis, we in-depth phenotyped colonic plasma cell (PC) differentiation at single-cell level, linked to ex vivo functional characterization experimental mouse models. Despite expanded B cells,...
Bone marrow plasma cells have been reported to represent a major source of IL-10; however, the impact cell derived IL-10 in that tissue remains poorly understood. We confirm this study even absence acute immune reactions, mature dominant IL-10+ population bone marrow, and identify myeloid-lineage as main local target for IL-10. Using Vert-X transcriptional reporter mice, we found more than 50% all were CD138+ cells, while other B lineage nearly absent organ. Accordingly, was supernatants...
Complement and the kallikrein-kinin cascade system are both activated in injured tissues. Little is known about their partnership immunopathogenesis of Chagas disease, chronic infection caused by intracellular protozoan Trypanosoma cruzi. In this study, we show that pharmacological targeting C5a receptor (C5aR) or bradykinin B2 (B2R) inhibited plasma leakage hamster cheek pouch topically exposed to tissue culture trypomastigotes (TCTs). Further, angiotensin-converting enzyme inhibitors...
During the course of Chagas disease, infectious forms Trypanosoma cruzi are occasionally liberated from parasitized heart cells. Studies performed with tissue culture trypomastigotes (TCTs, Dm28c strain) demonstrated that these parasites evoke neutrophil/CXCR2-dependent microvascular leakage by activating innate sentinel cells via toll-like receptor 2 (TLR2). Upon plasma extravasation, proteolytically derived kinins and C5a stimulate immunoprotective Th1 responses cross-talk between...
Mutated and unmutated IgE IgG play different partly opposing roles in allergy development, but the mechanisms controlling their relative production are incompletely understood. Here, we analyzed IgE-response murine food allergy. Deep sequencing of complementary-determining region (CDR) repertoires indicated that an ongoing extrafollicular response coexists with a germinal center response, even after long-lasting allergen challenges. Despite overall IgG1-dominance, significant proportion...
Abstract Paneth cells (PCs), a subset of intestinal epithelial (IECs) found at the base small crypts, play an essential role in maintaining homeostasis. Altered PCs function is associated with diverse pathologies, including ileal Crohn’s disease (CD). CD patients involvement have been previously demonstrated to display impairment and decreased levels anti-microbial peptides. Although immunosuppressive drug Azathioprine (AZA) widely used therapy, impact AZA on IEC differentiation remains...
Inhibitors of serine peptidases (ISPs) expressed by Leishmania major enhance intracellular parasitism in macrophages targeting neutrophil elastase (NE), a protease that couples phagocytosis to the prooxidative TLR 4 /PKR pathway. Here we investigated functional interplay between ISP-expressing L. and kallikrein-kinin system (KKS). Enzymatic assays showed NE inhibitor or recombinant ISP-2 inhibited KKS activation human plasma activated dextran sulfate. Intravital microscopy hamster cheek...
Food allergies are common, costly and potentially life-threatening disorders. They driven by Th2, but inhibited Th1 reactions. There is also evidence indicating that IL-2 agonist treatment inhibits allergic sensitization through expansion of regulatory T cells. Here, we tested the impact an in a novel model for food allergy to hen´s egg mice sensitized without artificial adjuvants. Prophylactic expanded Treg populations allergen-specific sensitization. However, already increased mast cell...
Allergic asthma is a chronical pulmonary disease with high prevalence. It manifests as maladaptive immune response to common airborne allergens and characterized by airway hyperresponsiveness, eosinophilia, type 2 cytokine-associated inflammation, mucus overproduction. Alveolar macrophages (AMs), although contributing lung homeostasis tolerance at steady state, have attracted less attention compared professional antigen-presenting adaptive cells in their contributions. Using an acute model...
Resumo-Este trabalho apresenta um método de detecção anomalias em vídeo baseado na decomposição dos seus quadros no subespaço componentes fundo gerado por dicionário.O dicionário utilizado é posto baixo proveniente da técnica RobustPCA.Os resultados mostram que essa implementação fornece resultado equivalente a outras técnicas RPCA Online já descritas literatura com tempo execução e complexidade muito inferiores.O também preserva imunidade à corrupção nos dados treinamento mantém desempenho...