A5011843447- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Autoimmune Bullous Skin Diseases
- Urticaria and Related Conditions
- vaccines and immunoinformatics approaches
- Genomics and Phylogenetic Studies
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Metabolism and Genetic Disorders
- Viral-associated cancers and disorders
- Cancer Genomics and Diagnostics
- Biochemical and biochemical processes
- Plant biochemistry and biosynthesis
- Immunotherapy and Immune Responses
- Lymphoma Diagnosis and Treatment
- Genomics and Rare Diseases
- Dermatology and Skin Diseases
- COVID-19 Clinical Research Studies
- Single-cell and spatial transcriptomics
- Nail Diseases and Treatments
- Forensic and Genetic Research
- Essential Oils and Antimicrobial Activity
- Long-Term Effects of COVID-19
- SARS-CoV-2 and COVID-19 Research
- Allergic Rhinitis and Sensitization
- Otitis Media and Relapsing Polychondritis
Institute for Integrative and Experimental Genomics
2019-2024
University of Lübeck
2014-2024
University Hospital Schleswig-Holstein
2023-2024
University Medical Center Hamburg-Eppendorf
2020
Universität Hamburg
2020
Inserm
2020
University of Cologne
2020
Roche (Switzerland)
2020
University of Rostock
2011-2014
Abstract Coronavirus disease 2019 (COVID-19) can damage cerebral small vessels and cause neurological symptoms. Here we describe structural changes in of patients with COVID-19 elucidate potential mechanisms underlying the vascular pathology. In brains severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-infected individuals animal models, found an increased number empty basement membrane tubes, so-called string representing remnants lost capillaries. We obtained evidence that brain...
Atopic dermatitis (AD) is a multifactorial inflammatory skin disease and an altered microbiota with increase of Staphylococcus aureus has been reported. However, the role fungi remains poorly investigated.We aimed to improve understanding fungal microbiota, mycobiota, in AD relation bacterial colonization.Skin swabs 16 patients healthy controls (HC) from four different sites, that antecubital crease, dorsal neck, glabella vertex multiple time points were analysed by DNA sequencing internal...
A small number of de novo assembled human genomes have been reported to date, and few complemented with population-based genetic variation, which is particularly important for North Africa, a region underrepresented in current genome-wide references. Here, we combine long- short-read whole-genome sequencing data recent assembly approaches into an Egyptian genome. The demonstrates well-balanced quality metrics variant phasing via linked reads haploblocks, associate gene expression changes...
The T cell receptor (TCR) determines specificity and affinity for both foreign self-peptides presented by the major histocompatibility complex (MHC). Although strength of TCR interactions with self-pMHC impacts function, it has been challenging to identify sequence features that predict fate. To discern patterns distinguishing TCRs from naive CD4+ cells low versus high self-reactivity, we used data 42 mice train a machine learning (ML) algorithm identifies population-level differences...
Abstract Post-COVID syndrome (PCS) currently affects approximately 3-17% of people following severe acute respiratory coronavirus 2 (SARS-CoV-2) infection and has the potential to become a significant global health burden. PCS presents with various symptoms, methods for improved assessment are presently developed guide therapy. Nevertheless, there few mechanistic insights treatment options. Here, we performed single-cell RNA transcriptomics on nasal biopsies from 33 patients suffering mild,...
Multiple myeloma is a bone marrow plasma cell tumor which supported by the external growth factors APRIL and IL-6, among others. Recently, we identified eosinophils megakaryocytes to be functional components of micro-environmental niches benign cells important local sources these cytokines. Here, investigated whether also support in MOPC315.BM model for multiple myeloma. As it was shown cells, IL-6 vitro, IL-5 had no effect. Depletion vivo blockade led reduction early load. Consistent with...
Mutations in mitochondrial DNA (mtDNA) lead to heteroplasmy, i.e., the intracellular coexistence of wild-type and mutant mtDNA strands, which impact a wide spectrum diseases but also physiological processes, including endurance exercise performance athletes. However, phenotypic consequences limited levels naturally arising heteroplasmy have not been experimentally studied date. We hence generated conplastic mouse strain carrying genome an AKR/J (B6-mtAKR) C57BL/6 J nuclear genomic...
Mutated and unmutated IgE IgG play different partly opposing roles in allergy development, but the mechanisms controlling their relative production are incompletely understood. Here, we analyzed IgE-response murine food allergy. Deep sequencing of complementary-determining region (CDR) repertoires indicated that an ongoing extrafollicular response coexists with a germinal center response, even after long-lasting allergen challenges. Despite overall IgG1-dominance, significant proportion...
Abstract CD8αα TCRαβ+ intestinal intraepithelial lymphocytes play a critical role in promoting homeostasis, although mechanisms controlling their development and peripheral homeostasis remain unclear. In this study, we examined the spatiotemporal of Bim thymic selection precursors fate these cells periphery. We found that T cell–specific expression during early/cortical, but not late/medullary, controls agonist limits private TCRβ repertoire. During process, agonist-selected double-positive...
Next generation sequencing (NGS) technologies enable studies and analyses of the diversity both T B cell receptors (TCR BCR) in human animal systems to elucidate immune functions health disease. Over last few years, several algorithms tools have been developed support respective raw data repertoire. These focus on distinct aspects processing require a strong bioinformatics background. To facilitate analysis repertoires by less experienced users, software is needed that combines most common...
Hematologic malignancies are currently underrepresented in multidisciplinary molecular-tumor-boards (MTB). This study assesses the potential of precision-oncology primary-refractory plasmablastic-lymphoma (prPBL), a highly lethal blood cancer.We evaluated clinicopathological and molecular-genetic data 14 clinically annotated prPBL-patients from initial diagnosis. For this proof-of-concept study, we employed our certified institutional MTB-pipeline...
Schnitzler syndrome is a rare autoinflammatory disorder characterized by urticarial rash, joint pain, recurrent fever, leucocytosis, elevated C-reactive protein (CRP) and serum amyloid A (SAA), monoclonal IgM or IgG gammopathy. According to the Strasbourg criteria , both rash gammopathy are mandatorily required for diagnosis of Schnitzler’s syndrome. However, incomplete variants lacking either skin symptoms have also been described. Here, we report case in which Schnitzler-like was made...
Abstract Several lines of evidence suggest that neurological symptoms in COVID-19 patients are partially due to damage small vessels. However, the potential mechanisms unclear. Here, we show brain endothelial cells express SARS-CoV-2 receptors. The main protease (M pro ) cleaves NEMO, essential modulator NF-κB signaling. By ablating M induces death human and a microvascular pathology mice is similar what find patients. Importantly, inhibition receptor-interacting protein kinase (RIPK) 3,...
Mucous membrane pemphigoid (MMP) is an autoimmune blistering disease characterized by autoantibodies against the basal zone of skin and surface-close epithelia predominant mucosal lesions. The oral cavity conjunctivae are most frequently affected, albeit clinical manifestations can also occur on skin. MMP-associated lesions outside typically lead to scarring. Mechanisms underlying scarring largely unknown in MMP effective treatment options limited. Herein, we assessed collagen architecture...
CD154 is a transmembrane cytokine expressed transiently on activated CD4 T cells upon cell receptor stimulation that interacts with CD40 antigen presenting cells. The signaling via CD154:CD40 essential for B maturation and germinal center formation, but also the final differentiation of during T-dependent humoral immune responses. Recent data demonstrate critically involved in selection clones negative process thymus. Whether influences repertoire peripheral responses has not yet been...
Bullous pemphigoid (BP) is the most prevalent autoimmune skin blistering disease and characterized by generation of autoantibodies against hemidesmosomal proteins BP180 (type XVII collagen) BP230. Most intriguingly, BP distinct from other diseases because it predominantly affects elderly individuals above age 75 years, raising question why clinical lesions emerges mostly in this later stage life, even harboring known putative BP-associated germline gene variants. The mitochondrial genome...
Autoimmune diseases develop over years - starting from a subclinical phenotype to clinically manifest autoimmune disease. The factors that drive this transition are ill-defined. To predict the turning point towards clinical disease and intervene in progress of autoimmune-mediated dysfunction, establishment new biomarkers is needed. Especially CD4 T cells crucially involved autoimmunity: first, during initiation phase, because they lose their tolerance self-peptides, second, by subsequent...
Mitochondria are maternally inherited cell organelles with their own genome, and perform various functions in eukaryotic cells such as energy production cellular homeostasis. Due to inheritance manifold biological roles health disease, mitochondrial genetics serves a dual purpose of tracing the history well disease susceptibility human populations across globe. This work requires comprehensive catalogue commonly observed genetic variations DNAs for all regions throughout world. So far,...
There is growing evidence supporting multidisciplinary molecular tumor boards (MTB) in solid tumors whereas hematologic malignancies remain underrepresented this regard. The present study aimed to assess the clinical relevance of MTBs primary refractory diffuse large B-cell lymphomas/high-grade lymphomas with MYC and BCL2 rearrangements (prDLBCL/HGBL-MYC/BCL2) (n = 13) HGBL, not otherwise specified (NOS), BCL6 (prHGBL, NOS-MYC/BCL6) 6) based on our previously published whole-exome sequencing...
Introduction Primarily driven by autoreactive B cells, pemphigus foliaceus (PF) is an uncommon autoimmune blistering skin disease of sporadic occurrence worldwide. However, PF reaches a prevalence 3% in the endemic areas Brazil, highest ever registered for any disease, which indicates environmental factors influencing immune response susceptible individuals. We aimed to provide insights into repertoire patients with living region compared healthy individuals from and non-endemic area....
Immunological differences between hosts, such as diverse TCR repertoires, are widely credited for reducing the risk of pathogen spread and adaptation in a population. Within-host immunological diversity might likewise be important robust control, but to what extent naive repertoires differ across different locations same host is unclear. T cell zones (TCZs) secondary lymphoid organs provide secluded microenvironmental niches. By harboring distinct TCRs, niches could enhance within-host...