A5052454459- Dermatology and Skin Diseases
- Gut microbiota and health
- Antibiotic Resistance in Bacteria
- Gastrointestinal motility and disorders
- Eating Disorders and Behaviors
- Autoimmune Bullous Skin Diseases
- Clostridium difficile and Clostridium perfringens research
- Cancer-related molecular mechanisms research
- Urticaria and Related Conditions
- Mycobacterium research and diagnosis
- Pediatric health and respiratory diseases
- Mitochondrial Function and Pathology
- Metabolism and Genetic Disorders
- Digestive system and related health
- ATP Synthase and ATPases Research
- Genetic Mapping and Diversity in Plants and Animals
- MicroRNA in disease regulation
- Helicobacter pylori-related gastroenterology studies
- Cancer Research and Treatments
- Genetic Neurodegenerative Diseases
- Galectins and Cancer Biology
- Animal Behavior and Welfare Studies
- Parasitic Infections and Diagnostics
- RNA regulation and disease
- Microscopic Colitis
Research Center Borstel - Leibniz Lung Center
2021-2025
Max Planck Institute for Evolutionary Biology
2016-2023
Kiel University
2016-2023
Evolutionary Genomics (United States)
2017
Czech Academy of Sciences, Institute of Experimental Medicine
2017
IBS is a common gut disorder of uncertain pathogenesis. Among other factors, genetics and certain foods are proposed to contribute. Congenital sucrase-isomaltase deficiency (CSID) rare genetic form disaccharide malabsorption characterised by diarrhoea, abdominal pain bloating, which features IBS. We tested (SI) gene variants for their potential relevance in IBS.We sequenced SI exons seven familial cases, screened four CSID mutations (p.Val557Gly, p.Gly1073Asp, p.Arg1124Ter p.Phe1745Cys)...
The gut microbiota influences intestinal barrier integrity through mechanisms that are incompletely understood. Here we show the commensal weakens by suppressing epithelial neuropilin-1 (NRP1) and Hedgehog (Hh) signaling. Microbial colonization of germ-free mice dampens signaling Hh pathway Toll-like receptor (TLR)-2, resulting in decreased NRP1 protein levels. Following activation via TLR2/TLR6, NRP1, a positive-feedback regulator signaling, is lysosomally degraded. Conversely, elevated...
Gut microbiota are linked to metabolic function, body weight regulation, and brain behavioral changes. Alteration of gut is repeatedly demonstrated in adults with anorexia nervosa (AN) transplantation stool from adult patients AN reduces gain, food consumption efficiency germ-free mice. No similar data available for adolescents, who might differ due their shorter duration illness.Nineteen female adolescent at admission after short-term recovery were included a longitudinal study compared 20...
The house mouse is a key model organism in skin research including host-microbiota interactions, yet little known about the microbiota of free-living mice. It similarly unclear how closely laboratory mice, which typically live under exceptionally hygienic conditions, resemble ancestral state microbial variation wild. In this study, we sampled an area spanning 270 km2 south-west France and collected 203 wild Mus musculus domesticus. We profiled ear on standing active communities (DNA-based...
Abstract Phenotypic variation of quantitative traits is orchestrated by a complex interplay between the environment (e.g. diet) and genetics. However, impact gene-environment interactions on phenotypic mostly remains elusive. To address this, we feed 1154 mice an autoimmunity-prone intercross line (AIL) three different diets. We find that diet substantially contributes to variability unmasks additional genetic susceptibility trait loci (QTL). By performing whole-genome sequencing AIL founder...
Anorexia nervosa (AN) is an often chronic and debilitating psychiatric disease whose etiology not completely understood. Recently, a potential role of inflammation has emerged in other diseases, such as depression, PTSD schizophrenia. The first results adults with AN seemed to confirm low-grade proinflammatory state until recent studies presented more differential findings. Studying adolescents shorter illness duration fewer confounding factors might help elucidate the underlying...
MicroRNAs (miRNAs) are small endogenous non-coding RNAs that control genes at post-transcriptional level. They essential for development and tissue differentiation, such altered miRNA expression patterns linked to the pathogenesis of inflammation cancer. There is evidence genetically controlled similar transcription protein-coding previous studies identified quantitative trait loci (QTL) in liver. So far, little attention has been paid skin. Moreover, epistatic remains unknown. In this...
Recent studies highlight the utility of quantitative trait locus (QTL) mapping for determining contribution host genetics to interindividual variation in microbiota. We previously demonstrated that similar gut microbiota, abundances bacterial taxa skin are significantly influenced by genetic variation. In this study, we analyzed microbiota mice from 15th generation an advanced intercross line using a novel approach extending both 16S rRNA gene copy (DNA) and transcript (RNA) levels, which...
Bullous pemphigoid (BP) is the most common autoimmune blistering disease. It predominately afflicts elderly and significantly associated with increased mortality. The observation of age-dependent changes in skin microbiota as well its involvement other inflammatory disorders suggests that may play a role emergence BP blistering. We hypothesize microbial diversity might occur before disease lesions, thus could represent an early indicator risk. present study aims to investigate potential...
Mutations in mitochondrial DNA (mtDNA) lead to heteroplasmy, i.e., the intracellular coexistence of wild-type and mutant mtDNA strands, which impact a wide spectrum diseases but also physiological processes, including endurance exercise performance athletes. However, phenotypic consequences limited levels naturally arising heteroplasmy have not been experimentally studied date. We hence generated conplastic mouse strain carrying genome an AKR/J (B6-mtAKR) C57BL/6 J nuclear genomic...
Infectious disease is widely considered to be a major driver of evolution. A preponderance signatures balancing selection at blood group-related genes thought driven by inherent trade-offs in susceptibility disease. B4galnt2 subject long-term house mice, where two divergent allele classes direct alternative tissue-specific expression glycosyltransferase the intestine versus vessels. The vessel class leads prolonged bleeding times similar von Willebrand humans, yet has been maintained for...
Mammalian lungs comprise a complex microbial ecosystem that interacts with host physiology. Previous research demonstrates the environment significantly contributes to bacterial community structure in upper and lower respiratory tract. However, influence of genetics on makeup lung microbiota remains ambiguous, largely due technical difficulties related sampling, as well challenges inherent investigating low biomass communities. Thus, innovative approaches are warranted clarify host-microbe...
Abstract Background Pathogenic mycobacteria, such as the Mycobacterium tuberculosis complex (Mtbc), and non-tuberculous mycobacteria (NTMs) can cause severe chronic pulmonary infections. However, not all infected patients develop active disease. Yet, it is unclear whether certain key taxa in lung microbiome play a role pathogenesis of (TB) NTM disease (LD). Material methods We employed 16S rRNA amplicon sequencing (V3-V4) to characterize baseline bronchoalveolar lavage fluid from patient...
<title>Abstract</title> The increasing prevalence of antimicrobial resistance (AMR) poses significant challenges in clinical settings. In particular, early screening and detection colonization by multidrug-resistant organisms (MDROs) patients at admission is crucial. this context, the use metagenomics (mNGS) holds promise for fast untargeted diagnostic methods. Here, we aimed to evaluate long-term stability rectal microbiome accuracy mNGS comparison culture whole-genome sequencing (WGS)...
ABSTRACT Introduction Bullous pemphigoid (BP) is the most common autoimmune blistering disease. It predominately afflicts elderly and significantly associated with increased mortality. The observation of age-dependent changes in skin microbiota as well its involvement other inflammatory disorders suggests that may play a role emergence BP blistering. We hypothesize microbial diversity might occur before disease lesions, thus could represent an early indicator risk. Objectives present study...
Summary Mutations in mitochondrial DNA (mtDNA) lead to heteroplasmy, i.e. the intracellular coexistence of wild-type and mutant mtDNA strands, which impact a wide spectrum diseases but also physiological processes, including endurance exercise performance athletes. However, phenotypic consequences limited levels naturally-arising heteroplasmy have not been experimentally studied date. We hence generated conplastic mouse strain carrying genome AKR/J (B6-mt AKR ) together with C57BL/6J nuclear...