A5050422951- Autoimmune Bullous Skin Diseases
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Urticaria and Related Conditions
- Platelet Disorders and Treatments
- Skin and Cellular Biology Research
- Oral and gingival health research
- Nail Diseases and Treatments
- Genetic and rare skin diseases.
- Cancer and Skin Lesions
- Dermatological and Skeletal Disorders
- Eosinophilic Disorders and Syndromes
- Cell Adhesion Molecules Research
- Cutaneous lymphoproliferative disorders research
- Hair Growth and Disorders
- Dermatology and Skin Diseases
- Cardiovascular Effects of Exercise
- Allergic Rhinitis and Sensitization
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Infectious Diseases and Mycology
- Psoriasis: Treatment and Pathogenesis
- Autoimmune and Inflammatory Disorders
- Lipid metabolism and disorders
- Immunodeficiency and Autoimmune Disorders
- Hedgehog Signaling Pathway Studies
- Electrolyte and hormonal disorders
Kurume University
2016-2025
University of Lübeck
2015-2019
Kyushu University
2018
Kobe University
2014
Kansai University
2014
Huazhong University of Science and Technology
2013
Weatherford College
2013
Oita Prefectural Hospital
2010
Kyushu University Hospital
2006
Hizen Psychiatric Center
2003
Anti-p200 pemphigoid has been characterized by autoantibodies to an unidentified 200-kDa protein (p200) of the dermal−epidermal junction. The objective this study was identify p200. We performed 2D gel electrophoresis dermal extracts and immunoblotting with patients' sera, followed MS analysis a unique band. band corresponded laminin γ1. Anti-laminin γ1 mAb reacted anti-p200 immunoprecipitates immunoblotting. Sera from 32 patients showed 90% reactivity recombinant products None healthy...
Although there are many reports of sporadic patients with paraneoplastic pemphigus (PNP), only a few systematic studies on large cohorts PNP have been reported.To analyse the clinical and immunological findings in cohort PNP.This retrospective study consisted 104 PNP. Clinical histopathological manifestations, associated neoplasms, complicating diseases, prognosis results immunofluorescence, immunoblotting enzyme-linked immunosorbent assays (ELISAs) were analysed.The this generally similar...
Rituximab is a promising therapy in pemphigus. However, there no consensus on optimum dose.To compare the efficacy, terms of clinical and immunological outcomes patients with pemphigus, high (2 × 1000 mg) vs. low dose 500 rituximab.This was randomized, observer-blinded trial wherein 22 pemphigus were randomized into two treatment groups. Patients received either doses (day 0 day 15) mg rituximab or rituximab, followed up for 48 weeks. Clinical activity assessed by blinded investigator....
Despite the established pathogenic role of anti‐desmoglein (Dsg) antibodies in classical pemphigus, significance autoantibodies to another desmosomal cadherin, desmocollin (Dsc) is at present unknown. No consistent immunoassay for immunoglobulin (Ig) G Dscs has been developed. The aim this study was develop reliable assays detect anti‐Dsc autoantibodies. We expressed soluble recombinant proteins (RPs) human Dsc1–3 mammalian cells and examined sera various types including 79 paraneoplastic...
Epitope spreading is involved in inducing and maintaining self-reactivity. pemphigus vulgaris (PV), caused by IgG autoantibodies to desmoglein 3 (Dsg3) Dsg1, was previously analyzed using Dsg3/Dsg1 extracellular domain–swapped molecules. However, precise identification of the responsible epitopes each molecule only this method problematic. In study, we studied epitope PV a novel immunoprecipitation–immunoblot Dsg3 (or Dsg1)/Dsg2 molecules, which overcomes problems associated with previous...
Mucocutaneous blistering is characteristic of autoimmune bullous dermatoses (AIBD). Blisters are caused by autoantibodies directed against structural components the skin. Hence, detection specific has become a hallmark for AIBD diagnosis. Studies on prevalence in healthy individuals yielded contradictory results.To clarify this, samples from 7063 blood donors were tested presence anti-BP180-NC16A, anti-BP230 and anti-Dsg1/3 IgG indirect immunofluorescence (IF) microscopy using...
Paraneoplastic pemphigus (PNP) shows autoantibodies mainly to plakin and desmosomal cadherin family proteins. We have recently identified alpha-2-macroglobulin-like-1 (A2ML1), a broad range protease inhibitor, as unique PNP antigen. In this study, we tested large number of sera by various methods. Forty (69.0%) 58 recognized A2ML1 recombinant protein expressed in COS7 cells immunofluorescence (IF) and/or immunoprecipitation (IP)/immunoblotting (IB). IP/IB showed higher sensitivity than IF....
Journal Article Clinical and immunological profiles of 25 patients with pemphigoid gestationis Get access N. Tani, Tani Department Dermatology Kurume University School Medicine, Institute Cutaneous Cell Biology 67 Asahimachi Fukuoka 830‐0011 Japan Search for other works by this author on: Oxford Academic Google Scholar Y. Kimura, Kimura H. Koga, Koga T. Kawakami, Kawakami C. Ohata, Ohata Ishii, Ishii Hashimoto Correspondence Takashi Hashimoto. E‐mail: hashimot@med.kurume-u.ac.jp British...
To confirm that sera from some BP patients reactive exclusively to the BP230 and study clinical immunological characteristics of this condition. were divided into three groups: only (BP230-BP), both BP180 (BP180-BP230-BP) (BP180-BP), based on results standard ELISAs for BP230. Clinical features statistically analyzed among groups. Then, targeted epitopes in each group studied by immunoblotting novel using domain-specific recombinant proteins. Forty-one, 65 47 153 categorized as BP230-BP,...
Abstract Phenotypic variation of quantitative traits is orchestrated by a complex interplay between the environment (e.g. diet) and genetics. However, impact gene-environment interactions on phenotypic mostly remains elusive. To address this, we feed 1154 mice an autoimmunity-prone intercross line (AIL) three different diets. We find that diet substantially contributes to variability unmasks additional genetic susceptibility trait loci (QTL). By performing whole-genome sequencing AIL founder...
Autoantibodies, including anti-BP180 and anti-BP230 antibodies in bullous pemphigoid (BP) mucous membrane (MMP), anti-type VII collagen (COL7) epidermolysis bullosa acquisita (EBA), are well characterized. Enzyme-linked immunosorbent assays (ELISAs) for the detection of these serum valuable diagnosis. Previous studies have indicated that blister fluid saliva can be detected using ELISA. The aim this study was to detect anti-BP180, anti-BP230, anti-COL7 several types samples, fluid, an...
Patients with autoimmune bullous disease have their quality of life (QOL) affected by both the and its treatment burden. While QOL assessment is clinically important, it often hindered limited time in clinical practice, highlighting need for accurate efficient evaluation tools. However, no validated questionnaires are currently available Japan. This study evaluated validity reliability Japanese versions Autoimmune Bullous Disease Quality Life (ABQOL) Treatment (TABQOL) questionnaires, as...
Although many new disease entities of autoimmune bullous (AIBD) have recently been recognized, satisfactory immunological diagnostic methods and comprehensive classifications for various AIBDs not established. To identify diagnostics AIBDs. We selected examined 4774 patients with from our cohort 5063 difficult AIBDs, whose sera information were sent method other institutes in either Japan or countries over the last 19 years. by including immunofluorescence, immunoblotting enzyme‐linked...