A5005077808- Mesenchymal stem cell research
- Hematopoietic Stem Cell Transplantation
- Acute Myeloid Leukemia Research
- Blood transfusion and management
- Cancer Cells and Metastasis
- Cell Adhesion Molecules Research
- Blood donation and transfusion practices
- Tissue Engineering and Regenerative Medicine
- CAR-T cell therapy research
- Protein Degradation and Inhibitors
- Immune Response and Inflammation
- Immune cells in cancer
- Chemokine receptors and signaling
- Blood groups and transfusion
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Pluripotent Stem Cells Research
- Virus-based gene therapy research
- Erythrocyte Function and Pathophysiology
- Platelet Disorders and Treatments
- Immune Cell Function and Interaction
- Angiogenesis and VEGF in Cancer
- Hemoglobinopathies and Related Disorders
- Retinoids in leukemia and cellular processes
- Acute Lymphoblastic Leukemia research
Leipzig University
2021-2025
University Hospital Leipzig
2019-2024
Cleveland Clinic
2023
Institute for Transfusion Medicine
2009-2021
Swiss Red Cross
2016-2018
Ludwig-Maximilians-Universität München
2012-2018
LMU Klinikum
2014-2018
German Red Cross
2007-2016
Goethe University Frankfurt
2007-2016
DRK-Blutspendedienst Baden-Württemberg - Hessen
2004-2015
Abstract Spontaneous and experimental metastasis can be effectively inhibited by the widely used anticoagulant heparin in different tumor models. At cellular level, many of antimetastatic effects vivo are due to its action on P-selectin-mediated binding. Whereas previous attention has focused P-selectin-dependent tumor-cell–platelet interactions blood-borne metastasis, we sought address potential contribution endothelial P-selectin expression adhesive events between microvasculature melanoma...
Background In vitro proliferative and differentiation potential of mesenchymal stromal cells generated from CD271+ bone marrow mononuclear (CD271-mesenchymal cells) has been demonstrated in several earlier recent reports. the present study we focused, addition to potential, on vivo immunosuppressive lymphohematopoietic engraftment-promoting these compared marrow-derived by plastic adherence (plastic adherence-mesenchymal cells).Design Methods We set up a series experimental protocols order...
Abstract Our study examined whether human bone marrow‐derived MSCs are able to differentiate, in vitro , into functional epithelial‐like cells. were isolated from the sternum of 8 patients with different hematological disorders. The surface phenotype these cells was characterized.To induce epithelial differentiation, cultured using Epidermal Growth Factor, Keratinocyte Hepatocyte Factor and Insulin‐like growth Factor‐II. Differentiated further characterized both morphologically functionally...
Cell therapy is a promising option for the treatment of acute or chronic myocardial ischemia. The intracoronary infusion cells imposes potential risk cell clotting, which may be prevented by addition anticoagulants. However, comprehensive analysis effects anticoagulants on function missing.Here, we investigated heparin and thrombin inhibitor bivalirudin bone marrow-derived mononuclear (BMC) functional activity homing capacity.Heparin, but not profoundly dose-dependently inhibited basal...
Therapeutic approaches using multipotent mesenchymal stromal cells (MSCs) are advancing in regenerative medicine, transplantation, and autoimmune diseases. The mechanisms behind MSC immune modulation still poorly understood the prediction of modulatory potential single preparations remains a major challenge for possible clinical applications. Here, we highlight galectin-9 (Gal-9) as novel, important modulator expressed by MSCs, which is strongly upregulated upon activation interferon-γ...
Summary Ample evidence suggests that many of the in vivo anti-metastatic effects by heparins reflect their actions on P-selectin-mediated binding. We hypothesized ability widely used and derivatives to interfere with P-selectin-dependent tumour cell interactions under flow vitro could be identify anticoagulants advanced inhibitory functions experimental blood-borne metastasis vivo. To test this assumption, impact unfractionated heparin, low-molecular-weight (LMWH) nadroparin enoxaparin,...
Although mesenchymal stromal cells (MSCs) are being increasingly used as cell therapeutics in clinical trials, the mechanisms that regulate their chemotactic migration behavior incompletely understood. We aimed to better define ability of GTPase regulator cytoskeletal activation, Rho, modulate induction MSCs a transwell chemotaxis assay. found culture-expanded migrate poorly toward exogenous phospholipids lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P) assays. Moreover,...
Stem cells play an important role in the pathogenesis and maintenance of most malignant tumors. Acute myeloid leukemia (AML) is a stem cell disease. The inefficient targeting leukemic (LSC) considered responsible for relapse after induction complete hematologic remission (CR) AML. promyelocytic (APL) subtype AML characterized by t(15;17) translocation expression PML/RARalpha fusion protein. Treatment APL with all-trans retinoic acid (ATRA) induces CR, but not molecular (CMR), because...
Mucocutaneous blistering is characteristic of autoimmune bullous dermatoses (AIBD). Blisters are caused by autoantibodies directed against structural components the skin. Hence, detection specific has become a hallmark for AIBD diagnosis. Studies on prevalence in healthy individuals yielded contradictory results.To clarify this, samples from 7063 blood donors were tested presence anti-BP180-NC16A, anti-BP230 and anti-Dsg1/3 IgG indirect immunofluorescence (IF) microscopy using...
Intravenously transplanted mesenchymal stromal cells (MSCs) have been shown to interact with endothelial and migrate tissues. However, intracellular signals regulating MSC migration are still incompletely understood. Here, we analyzed the role of Rap1 GTPase in human bone marrow-derived MSCs vitro short-term homing mice vivo. expressed both Rap1A Rap1B mRNAs, which were downregulated after treatment siRNA against and/or B. In a flow chamber model pre-established umbilical vein (HUVECs),...