A5002583105- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Antibiotic Resistance in Bacteria
- Cancer Research and Treatments
- Immunotherapy and Immune Responses
- Cytomegalovirus and herpesvirus research
- Parasitic Infections and Diagnostics
- HIV Research and Treatment
- Mycobacterium research and diagnosis
- SARS-CoV-2 and COVID-19 Research
- Bacteriophages and microbial interactions
- Cell Adhesion Molecules Research
- Non-Destructive Testing Techniques
- Vibrio bacteria research studies
- Virus-based gene therapy research
- HIV/AIDS drug development and treatment
- Enzyme Production and Characterization
- Traumatic Brain Injury and Neurovascular Disturbances
- Cell death mechanisms and regulation
- Cerebrospinal fluid and hydrocephalus
Medizinische Hochschule Hannover
1989-2024
German Center for Infection Research
2019-2023
University Medical Center Freiburg
2015-2017
University of Freiburg
2017
Institute of Medical Microbiology and Hygiene
2015
La Jolla Institute for Immunology
2010-2014
Ruhr University Bochum
2012
Human cytomegalovirus (HCMV) establishes lifelong infection with recurrent episodes of virus production and shedding despite the presence adaptive immunological memory responses including HCMV immune immunoglobulin G (IgG). Very little is known how evades from humoral cellular IgG-dependent responses, latter being executed by cells expressing surface receptors for Fc domain IgG (FcγRs). Remarkably, expresses RL11-encoded gp34 UL119-118-encoded gp68 type I transmembrane glycoproteins which...
The ectodomain of matrix protein 2 is a universal influenza A virus vaccine candidate that provides protection through antibody-dependent effector mechanisms. Here we compared the functional engagement Fcγ receptor (FcγR) family members by two M2e-specific monoclonal antibodies (MAbs), MAb 37 (IgG1) and 65 (IgG2a), which recognize similar epitope in M2e with affinities. binding to virus-infected cells triggered all three activating mouse receptors vitro, whereas activated only FcγRIII....
Signaling through TLR2 promotes inflammation and modulates CD4(+) CD25(+) Tregs. We assessed mechanistically how this molecule would alter immunoregulation in type 1 diabetes (T1D). also asked whether may be involved our recent discovery that viral infection can protect from autoimmune by expanding invigorating Treatment of prediabetic mice with a synthetic agonist diminished T1D increased the number function Tregs, conferring DCs tolerogenic properties. ligation promoted expansion Tregs...
Abstract The importance of Fc-dependent effector functions Abs induced by vaccination is increasingly recognized. However, mice against HIV envelope (Env) a skewed Th cell response leading to Env-specific with reduced function. To overcome this bias, GagPol-specific cells were harnessed provide intrastructural help for B after immunization virus-like particles containing GagPol and Env. This led balanced humoral immune more inflammatory Fc glycan profile. increased quality in the Ab Env was...
Abstract One of the requirements for efficient vaccination against infection is to achieve best combination an adequate adjuvant with antigenic information deliver. Although plasmid DNA a promising tool bearing unique potential activate humoral and cellular immunity, actual challenge increase immunogenicity in human protocols which efficacy has proven rather limited. Previous work showed that bacterial backbone potent properties because it contains CpG motifs are particular activating...
Acute resolving viral infections are often associated with a strong and multi-specific T-cell response, whereas in persistent responses impaired. It has been suggested that the resuscitation of antiviral response could be powerful tool to target persisting viruses. Several immunoregulatory pathways, such as IL-10 TGF-β, have shown involved induction exhaustion persistence. In this study, we sought investigate whether TGF-β signaling is also relevant maintenance after persistence established,...
Acute or chronic viral infections can lead to generalized immunosuppression. Several mechanisms, such as immunopathology of CD8(+) T cells, inhibitory receptors, regulatory (Treg) contribute immune dysfunction. Moreover, patients with usually do not respond vaccination, a finding that has been previously explained. Recently, we reported CD169(+) macrophages enforce replication, which is essential for guaranteeing antigen synthesis and efficient adaptive responses. In the present study, used...
Intestinal fibrosis is a common and serious complication of Crohn's disease characterized by the accumulation fibroblasts, deposition extracellular matrix, formation scar tissue. Although many factors including cytokines proteases contribute to development intestinal fibrosis, initiating mechanisms complex interplay between these remain unclear.Chronic infection mice with Salmonella enterica serovar Typhimurium was used induce fibrosis. A murine protease-specific CLIP-CHIP microarray...
Background During the RNA encapsidation process of human immunodeficiency virus (HIV) viral genomic, unspliced (gRNA) is preferentially incorporated into assembling virions. However, a certain amount spliced transcripts can also be detected in particles. Recently, we observed that nuclear export HIV and lentiviral vector gRNA by Rev required for efficient encapsidation. Since singly-spliced contain Rev-response element (RRE), investigated if efficiency RRE-containing HIV-vector increased...
Abstract Infection of mammalian cells with viruses often induces apoptosis. How the recognition leads to apoptosis infected cell and which host factors regulate this death is incompletely understood. In study, we focussed on two major anti-apoptotic proteins cell, whose abundance activity are important for survival, Bcl-2-like Mcl-1 Bcl-X L . During infection epithelial fibroblasts modified vaccinia virus Ankara (MVA), protein levels dropped but MVA F1L could replace functionally; a similar...
Infectious disease is widely considered to be a major driver of evolution. A preponderance signatures balancing selection at blood group-related genes thought driven by inherent trade-offs in susceptibility disease. B4galnt2 subject long-term house mice, where two divergent allele classes direct alternative tissue-specific expression glycosyltransferase the intestine versus vessels. The vessel class leads prolonged bleeding times similar von Willebrand humans, yet has been maintained for...
The devastating impact of COVID-19 on global health shows the need to increase our pandemic preparedness. Recombinant therapeutic antibodies were successfully used treat and protect at-risk patients from COVID-19. However, currently circulating Omicron subvariants SARS-CoV-2 are largely resistant antibodies, novel approaches generate broadly neutralizing urgently needed. Here, we describe a tetravalent bispecific antibody, A7A9 TVB, which actively neutralized many variants concern, including...
Elevated levels of systemic IL-10 have been associated with several chronic viral infections, including HCV, EBV, HCMV and LCMV. In the LCMV infection model, both elevated enhanced dendritic cells (DCs) are important for persistence. This report highlights relationship between tropism DCs induction in CD4 T cells, which we identify as most frequent IL-10-expressing cell type infection. Here that infected CD8αneg express IL-10, induce expression specific suppress LCMV-specific proliferation....
Human CD81 and CD9 are members of the tetraspanin family proteins characterized by a canonical structure four transmembrane domains two extracellular loop domains. Tetraspanins known as molecular facilitators, which assemble organize cell surface receptors partner molecules forming clusters tetraspanin-enriched microdomains. They have been implicated to play various biological roles including an involvement in infections with microbial pathogens. Here, we demonstrate important role for...