A5015811382- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Carbohydrate Chemistry and Synthesis
- Liver Disease Diagnosis and Treatment
- Galectins and Cancer Biology
- SARS-CoV-2 and COVID-19 Research
- Transgenic Plants and Applications
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Tuberculosis Research and Epidemiology
- Fungal and yeast genetics research
- Enzyme Production and Characterization
- Cancer Research and Treatments
- Hepatitis C virus research
- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- Liver Disease and Transplantation
- Mycobacterium research and diagnosis
- Cellular transport and secretion
- Viral gastroenteritis research and epidemiology
- Organ Transplantation Techniques and Outcomes
- Toxin Mechanisms and Immunotoxins
- Cancer Genomics and Diagnostics
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
Ghent University
2016-2025
AZ Groeninge
2015-2025
Eurogentec (Belgium)
2025
Vlaams Instituut voor Biotechnologie
2009-2024
VIB-UGent Center for Medical Biotechnology
2015-2024
Cancer Research Institute Ghent
2019-2024
University of Liège
2023
KU Leuven
2023
Universitair Ziekenhuis Leuven
2023
Antwerp University Hospital
2023
An HEK293S cell line resistant to ricin was prepared by mutagenesis using ethyl methanesulfonate. It shown lack N -acetylglucosaminyltransferase I (GnTI) activity, and consequently unable synthesize complex N-glycans. The tetracycline-inducible opsin expression system assembled into this GnTI − line. Stable lines were isolated that gave tetracycline/sodium butyrate-inducible of the WT gene at levels comparable with those observed in parent Analysis N-glycan rhodopsin expressed stable showed...
The HEK293 human cell lineage is widely used in biology and biotechnology. Here we use whole-genome resequencing of six 293 lines to study the dynamics this aneuploid genome response manipulations generate common derivatives, such as transformation stable clone generation (293T); suspension growth adaptation (293S); cytotoxic lectin selection (293SG). Remarkably, observe that copy number alteration detection could identify genomic region enabled survival under selective conditions (i.c....
Pichia pastoris has been a workhorse of protein production for decades. De Schutter et al. present its genomic sequence, which will allow development improved strains. The methylotrophic yeast is widely used the proteins and as model organism studying peroxisomal biogenesis methanol assimilation. P. strains capable human-type N-glycosylation are now available, increases utility this biopharmaceutical production. Despite biotechnological importance, relatively few genetic tools or engineered...
Necrostatin-1 (Nec-1) is widely used in disease models to examine the contribution of receptor-interacting protein kinase (RIPK) 1 cell death and inflammation. We studied three Nec-1 analogs: Nec-1, active inhibitor RIPK1, inactive (Nec-1i), its variant, stable (Nec-1s), more variant. report that identical methyl-thiohydantoin-tryptophan, an potent immunomodulatory enzyme indoleamine 2,3-dioxygenase (IDO). Both Nec-1i inhibited human IDO, but Nec-1s did not, as predicted by molecular...
Emergence of SARS-CoV-2 causing COVID-19 has resulted in hundreds thousands deaths. In search for key targets effective therapeutics, robust animal models mimicking humans are urgently needed. Here, we show that Syrian hamsters, contrast to mice, highly permissive and develop bronchopneumonia strong inflammatory responses the lungs with neutrophil infiltration edema, further confirmed as consolidations visualized by micro-CT alike clinical practice. Moreover, identify an exuberant innate...
Congenital disorders of glycosylation are genetic syndromes that result in impaired glycoprotein production. We evaluated patients who had a novel recessive disorder glycosylation, with range clinical manifestations included hepatopathy, bifid uvula, malignant hyperthermia, hypogonadotropic hypogonadism, growth retardation, hypoglycemia, myopathy, dilated cardiomyopathy, and cardiac arrest.Homozygosity mapping followed by whole-exome sequencing was used to identify mutation the gene for...
N-linked protein glycosylation is found in all domains of life. In eukaryotes, it the most abundant modification secretory and membrane proteins, process coupled to translocation folding. We that bacteria, N-glycosylation can occur independently machinery. an vitro assay, bacterial oligosaccharyltransferase glycosylated a folded endogenous substrate with high efficiency bovine ribonuclease A low efficiency. Unfolding eukaryotic greatly increased glycosylation. propose system, sites are...
The PglB oligosaccharyltransferase (OTase) of Campylobacter jejuni can be functionally expressed in Escherichia coli, and its relaxed oligosaccharide substrate specificity allows the transfer different glycans from lipid carrier undecaprenyl pyrophosphate to an acceptor protein. To investigate PglB, we tested a set lipid-linked polysaccharides E. coli Salmonella enterica serovar Typhimurium. A hexose linked C-6 monosaccharide at reducing end did not inhibit O antigen However, required...
ABSTRACT The Pichia pastoris N-glycosylation pathway is only partially homologous to the in human cells. In Golgi apparatus, cells synthesize complex oligosaccharides, whereas form mannose structures that can contain up 40 residues. This hypermannosylation of secreted glycoproteins hampers downstream processing heterologously expressed and leads production protein-based therapeutic agents are rapidly cleared from blood because presence terminal Here, we describe engineering P. produce...
The unfolded protein response (UPR) in eukaryotes upregulates factors that restore ER homeostasis upon folding stress and yeast is activated by a non-conventional splicing of the HAC1 mRNA. spliced mRNA encodes an active transcription factor binds to UPR-responsive elements promoter UPR target genes. Overexpression gene S. cerevisiae can reportedly lead increased production heterologous proteins. To further such studies biotechnology favored Pichia pastoris, we cloned characterized P....
The conserved oligomeric Golgi (COG) complex is a heterooctameric that regulates intraGolgi trafficking and the integrity of compartment in eukaryotic cells. Here, we describe patient with mild form congenital disorder glycosylation type II (CDG-II) caused by deficiency Cog1 subunit complex. This has defect both N- O-glycosylation. Mass spectrometric analysis structures N-linked glycans released from glycoproteins patient's serum revealed reduction sialic acid galactose residues. Peanut...
We evaluated the use of blood serum N-glycan fingerprinting as a tool for diagnosis hepatocellular carcinoma (HCC) in patients with cirrhosis induced by hepatitis B virus (HBV). A group 450 HBV-infected liver fibrosis or without HCC were studied. was diagnosed α-fetoprotein (AFP) analysis, ultrasonography, and/or computed tomography and studied histologically. profiles proteins determined DNA sequencer–based carbohydrate analytical profiling technology. In this study, we found that branch...
Many pro-inflammatory pathways leading to arthritis have global effects on the immune system rather than only acting locally in joints. The reason behind regional and patchy distribution of represents a longstanding paradox. Here we show that biomechanical loading acts as decisive factor transition from systemic autoimmunity joint inflammation. Distribution inflammation erosive disease is confined mechano-sensitive regions with unique microanatomy. Curiously, this pathway relies stromal...
The arbuscular mycorrhizal (AM) symbiosis represents the most widely distributed mutualistic root symbiosis. We report that extracts of plants contain a lipophilic signal capable inducing phosphate transporter genes StPT3 and StPT4 potato (Solanum tuberosum L.), are specifically induced in roots colonized by AM fungi. same caused rapid extracellular alkalinization suspension-cultured tomato lycopersicum L.) cells induction mycorrhiza-specific gene LePT4 these cells. active principle was...
Abstract A mutant of tomato (Solanum lycopersicum) with reduced abscisic acid (ABA) production (sitiens) exhibits increased resistance to the necrotrophic fungus Botrytis cinerea. This is correlated a rapid and strong hydrogen peroxide-driven cell wall fortification response in epidermis cells that absent normal ABA production. Moreover, basal expression defense genes higher compared wild-type tomato. Given importance this fast sitiens resistance, we investigated cuticle properties at...