A5064222111- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Chemical Synthesis and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Enzyme Production and Characterization
- Chemistry and Chemical Engineering
- Immunotherapy and Immune Responses
- Biochemical and Molecular Research
- Proteoglycans and glycosaminoglycans research
- Biotechnology and Related Fields
- Immune Cell Function and Interaction
- Advanced Biosensing Techniques and Applications
- Analytical Chemistry and Chromatography
- Enzyme Structure and Function
- Enzyme Catalysis and Immobilization
- Research on Leishmaniasis Studies
- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- RNA Interference and Gene Delivery
- Microbial Metabolic Engineering and Bioproduction
- Microbial Metabolites in Food Biotechnology
- Bacteriophages and microbial interactions
- Trypanosoma species research and implications
- Various Chemistry Research Topics
- Genetics, Bioinformatics, and Biomedical Research
Indiana University Bloomington
2014-2024
Indiana University
2016-2022
Bloomington Health Foundation
2016-2020
Iowa State University
2008-2019
Harvard University
2018
Institute for Advanced Study
2018
University of California, Davis
2016
University of Georgia
2016
University of California, San Diego
2016
Alfred P. Sloan Foundation
2008
The success of microarrays, such as DNA chips, for biosample screening with minimal sample usage has led to a variety technologies assays on glass slides. Unfortunately, small molecules, carbohydrates, these methods usually rely covalent bond formation, which requires unique functional handles and multiple chemical steps. A new simpler concept in microarray formation is based noncovalent fluorous-based interactions. fluorous tail designed not only aid saccharide purification but also allow...
The PglB oligosaccharyltransferase (OTase) of Campylobacter jejuni can be functionally expressed in Escherichia coli, and its relaxed oligosaccharide substrate specificity allows the transfer different glycans from lipid carrier undecaprenyl pyrophosphate to an acceptor protein. To investigate PglB, we tested a set lipid-linked polysaccharides E. coli Salmonella enterica serovar Typhimurium. A hexose linked C-6 monosaccharide at reducing end did not inhibit O antigen However, required...
Targeting pathogen recognition receptors on dendritic cells (DCs) offers the advantage of triggering specific signaling pathways to induce a tailored and robust immune response. In this work, we describe novel approach targeted antigen delivery by decorating surface polyanhydride nanoparticles with carbohydrates provide "pathogen-like" properties that ensure engage C-type lectin DCs. The was functionalized covalent linkage dimannose lactose residues using an amine–carboxylic acid coupling...
We report herein the first synthesis of linear and branched mannose oligosaccharides using fluorous-tag assistance with reagents FSPE protocols that are amenable to automation. The particular fluorous linker proved maintain solubility growing oligosaccharide chain such identical reaction solvent conditions purification could be used between glycosylation deprotection reactions, thereby rendering procedures
To examine the potential role of methanobactin (mb) as extracellular component a copper acquisition system in Methylosinus trichosporium OB3b, metal binding properties mb were examined. Spectral (UV−visible, fluorescence, and circular dichroism), kinetic, thermodynamic data suggested coordination changes at different Cu(II):mb ratios. Mb appeared to initially bind Cu(II) homodimer with comparatively high affinity ratios below 0.2, constant (K) greater than that EDTA (log K = 18.8) an...
The first automated solution-phase synthesis of β-1,4-mannuronate and β-1,4-mannan oligomers has been accomplished by using a β-directing C-5 carboxylate strategy. By utilizing fluorous-tag assisting purification after repeated reaction cycles, was synthesized up to hexasaccharide with limited loading glycosyl donor (up 3.5 equiv) for each glycosylation cycle due the homogeneous condition. After global reduction uronates, obtained, thereby demonstrating new approach β-mannan synthesis.
Diastereomeric adducts comprising an enantiomerically pure monosaccharide analyte, a peptide, and/or amino acid and divalent metal ion (for 16 different isomers) are generated by electrospray ionization analyzed combined mobility spectrometry-mass spectrometry (IMS-MS) techniques. Mobility distributions of [l-Ser + M H]+ (where l-Ser is l-serine given monosaccharide), [l-Phe-Gly l-Phe-Gly l-phenylalanine–glycine), [MnII (l-Phe-Gly – H) M]+ complex ions used to determine collision cross...
The first analytical method is presented for the identification and absolute configuration determination of all 24 aldohexose 2-ketohexose isomers, including D L enantiomers allose, altrose, galactose, glucose, gulose, idose, mannose, talose, fructose, psicose, sorbose, tagatose. Two unique fixed ligand kinetic combinations were discovered to create significant enough energetic differences achieve chiral discrimination among hexoses. Each these hexoses yields ratios a specific pair fragment...
Abstract Rapid development of antisense therapies can enable on-demand responses to new viral pathogens and make personalized medicine for genetic diseases practical. Antisense phosphorodiamidate morpholino oligomers (PMOs) are promising candidates fill such a role, but their challenging synthesis limits widespread application. To rapidly prototype potential PMO drug candidates, we report fully automated flow-based oligonucleotide synthesizer. Our optimized platform reduces coupling times by...
Fooling conA? Fluorous microarrays allow not only qualitative, but also quantitative assessment of binding to show that conA can accept modifications its usual mannose ligand at the C-6 position and bind bacterial seven-carbon analogues.
A straightforward method utilizing a bismuth(V) compound was developed for the activation of thiopropylglycosides coupling to various acceptors; good excellent yields were obtained without applying additional additives/co-promoters. The does not require low temperatures, is applicable wide variety carbohydrates, and tolerates different functional groups including alkenes.
This review highlights current techniques for carbohydrate purification and identifies research gaps.
De novo carbohydrate sequencing, including monosaccharide identification, largely remains a tremendous analytical challenge. A first step in the complete structural determination of any large polysaccharide is an accurate and robust method for analysis constituent monosaccharides. Herein, mass spectrometry-based identification absolute configuration all 12 pentose isomers, d l enantiomers arabinose, lyxose, ribose, xylose, ribulose, xylulose, reported. As compared to earlier work distinguish...
R1128 substances are anthraquinone natural products that were previously reported as non-steroidal estrogen receptor antagonists with <i>in vitro</i> and vivo</i>potency approaching of tamoxifen. From a biosynthetic viewpoint, these polyketides possess structurally interesting features such an unusual primer unit absent in the well studied anthracyclic tetracyclic products. The entire gene cluster was cloned expressed <i>Streptomyces lividans</i>, genetically developed heterologous host. In...
Carbohydrate microarrays can map out key interactions of carbohydrates and proteins in a high-throughput manner, but require the inclusion range sugars for their optimal use. Here we present synthesis use new hydroxylamine-modified fluorous tag that allows facile incorporation reducing into noncovalent fluorous-based microarray after simple purification by solid-phase extraction (FSPE). The supports quantitative screening against carbohydrate-binding proteins.