A5055380517- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Gut microbiota and health
- Immune Cell Function and Interaction
- Veterinary medicine and infectious diseases
- Clostridium difficile and Clostridium perfringens research
- Helicobacter pylori-related gastroenterology studies
- Microscopic Colitis
- Salmonella and Campylobacter epidemiology
- T-cell and B-cell Immunology
- Inflammatory Bowel Disease
- RNA Interference and Gene Delivery
- Mycobacterium research and diagnosis
- Advanced Drug Delivery Systems
- Pharmacological Effects of Natural Compounds
- Influenza Virus Research Studies
- Parasitic Infections and Diagnostics
- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Yersinia bacterium, plague, ectoparasites research
- Digestive system and related health
- Escherichia coli research studies
- Inhalation and Respiratory Drug Delivery
- Gastrointestinal motility and disorders
- Nanoparticle-Based Drug Delivery
Iowa State University
2015-2024
Case Western Reserve University
2021
Cornell University
2021
University of California, Davis
2021
University of Nebraska Medical Center
2019
General Department of Preventive Medicine
2010-2016
Massachusetts Institute of Technology
2015
Ames National Laboratory
2014
Korea University
2009
Veterinary Medical Teaching Hospital
1990-1997
Targeting pathogen recognition receptors on dendritic cells (DCs) offers the advantage of triggering specific signaling pathways to induce a tailored and robust immune response. In this work, we describe novel approach targeted antigen delivery by decorating surface polyanhydride nanoparticles with carbohydrates provide "pathogen-like" properties that ensure engage C-type lectin DCs. The was functionalized covalent linkage dimannose lactose residues using an amine–carboxylic acid coupling...
Despite the successes provided by vaccination, many challenges still exist with respect to controlling new and re-emerging infectious diseases. Innovative vaccine platforms composed of adaptable adjuvants able appropriately modulate immune responses, induce long-lived immunity in a single dose, deliver immunogens safe stable manner via multiple routes administration are needed. This work describes development novel biodegradable polyanhydride nanoparticle-based platform administered as...
Large animal models, such as the dog, are increasingly being used for studying diseases including gastrointestinal (GI) disorders. Dogs share similar environmental, genomic, anatomical, and intestinal physiologic features with humans. To bridge gap between commonly rodents, humans, expand translational potential of dog model, we developed a three-dimensional (3D) canine GI organoid (enteroid colonoid) system. Organoids have recently gained interest in research this model system better...
Abstract Inflammatory bowel disease (IBD) causes inflammation to the gastrointestinal tract. Local administration of anti‐inflammatory drugs such as 5‐aminosalicylic acid (5‐ASA) can alleviate symptoms IBD. The application nanoparticles for IBD treatment in direct rectal showed high drug availability and efficacy. However, relying on size‐dependent adsorption smaller particles is not sufficient making formulation capable targeting. Intestinal organoids improve functionality due their ability...
Intestinal organoids can be used as an ex vivo epithelial model to study different drug delivery effects on cells’ luminal surface. In this study, the impact of surface charge 5-ASA loaded PLGA nanoparticles into lumen was investigated. Alginate and chitosan were coat provide negative positive charges particles, respectively. The organoid growth viability not affected by presence either alginate- or chitosan-coated nanoparticles. It shown that could transported from serosal side dye...
Ulcerative colitis is a chronic condition in which dysregulated immune response contributes to the acute intestinal inflammation of colon. Current clinical therapies often exhibit limited efficacy and undesirable side effects. Here, programmable nanomicelles were designed for treatment loaded with RU.521, an inhibitor cyclic GMP-AMP synthase-stimulator interferon genes (cGAS-STING) pathway. STING-inhibiting micelles (SIMs) comprise hyaluronic acid-stearic acid conjugates include reactive...
Daily gastric intubation of lipopolysaccharide (LPS)-responsive C3H/HeN, BALB/c, and Swiss mice with SRBC for 2 wk resulted in oral tolerance, whereas similarly treated LPS-nonresponsive C3H/HeJ gave splenic anti-SRBC PFC responses, including the IgA isotype, after systemic challenge antigen. Oral tolerance LPS-responsive C3H/HeN was due to T suppressor (Ts) cells because significant Ts cell activity demonstrated both Peyer's patches (PP) spleens these animals. On other hand, from PP...
Infection with Helicobacter species has been associated the development of mucosal inflammation and inflammatory bowel disease (IBD) in several mouse models. However, consensus regarding role as a model organism to study microbial-induced IBD is confounded by presence complex colonic microbiota.To investigate kinetics effects immune system activation commensal bacteria following H bilis colonisation gnotobiotic mice.C3H/HeN mice harbouring an altered Schaedler flora (ASF) were selectively...
An opportunity exists today for cross-cutting research utilizing advances in materials science, immunology, microbial pathogenesis, and computational analysis to effectively design the next generation of adjuvants vaccines. This study integrates these into a bottom-up approach molecular nanoadjuvants capable mimicking immune response induced by natural infection but without toxic side effects. Biodegradable amphiphilic polyanhydrides possess unique ability mimic pathogens pathogen associated...
Early-weaned pigs (n = 64) averaging 5.3 ± 0.3 kg and distributed into two environments (dirty clean) were used to evaluate effects of conjugated linoleic acid (CLA) on growth performance, immune competence, empty body composition. A factorial (2 × 4) arrangement within a split-plot design, with four littermate as the experimental unit for environment, pig litter dietary treatment, d-0 weight covariate, in data analysis. Diets formulated contain CLA at 0, 0.67, 1.33, or 2% exceed NRC (1988)...
Abstract This study focuses on the development of single dose vaccines based biodegradable polyanhydride microspheres that have unique capability to modulate immune response mechanism. The polymer system employed consists copolymers 1,6‐bis( p ‐carboxyphenoxy)hexane and sebacic acid. Two copolymer formulations been shown provide extended release kinetics protein stability were investigated. Using tetanus toxoid (TT) as a model antigen, in vivo studies C3H/HeOuJ mice demonstrated...
The first line of defense against pathogens that enter the host by oral route appears to involve gut-associated lymphoreticular tissue—e.g., Peyer's patches (PP). Although animals can readily be immunized orally administered antigen mobilizes secretory immune system, there is a total lack local antibody synthesis in PP and cellular basis for this deficiency remains mystery. A cell population, obtained when murine were treated with neutral protease (Dispase), consisted accessory cells...
Abstract The release kinetics and stability of ovalbumin encapsulated into polyanhydride microspheres with varying chemistries were studied. Polymers based on the anhydride monomers sebacic acid (SA), 1,6‐bis( p ‐carboxyphenoxy)hexane (CPH), 1,8‐bis ( ‐carboxyphenoxy)‐3,6‐dioxaoctane (CPTEG) utilized. Microspheres fabricated using two non‐aqueous methods: a solid/oil/oil double emulsion technique cryogenic atomization. studies showed that fabrication methods did not significantly affect...